Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,714,757	G→T	R546R (CGC→CGA)	bisC ←	biotin sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,714,757	0	G	T	100.0%	177.6 / NA	53	R546R (CGC→CGA)	bisC	biotin sulfoxide reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/30);  total (23/30)

ATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAACATCAAAATCATTACGCGCTTCATAGC  >  NC_000913/3714622‑3714893                                                                                                                                        |                                                                                                                                         aTGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCt                                                                                                                                      >  2:355340/1‑140 (MQ=255)   gcgcCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGt                                                                                                                                    >  1:919214/1‑140 (MQ=255)   gcgcCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGt                                                                                                                                    >  1:550083/1‑140 (MQ=255)     gcCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCg                                                                                                                                  <  2:635954/140‑1 (MQ=255)       cAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCggg                                                                                                                                <  1:643578/140‑1 (MQ=255)         gCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCa                                                                                                                              <  1:784495/140‑1 (MQ=255)           ggATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCggg                                                                                                                                <  1:323031/136‑1 (MQ=255)           ggATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCggg                                                                                                                                >  2:323031/1‑136 (MQ=255)           ggATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAAc                                                                                                                            <  2:634184/140‑1 (MQ=255)               cgcgGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCaa                                                                                                                             >  1:819240/1‑135 (MQ=255)               cgcgGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCaa                                                                                                                             <  2:819240/135‑1 (MQ=255)               cgcgGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTta                                                                                                                        >  2:504265/1‑140 (MQ=255)                  ggCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATaa                                                                                                                     <  1:504265/140‑1 (MQ=255)                    cAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATaaaa                                                                                                                   <  2:80052/140‑1 (MQ=255)                       aaaTCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGt                                                                                                                >  2:9693/1‑140 (MQ=255)                         aTCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGttt                                                                                                              <  2:553055/140‑1 (MQ=255)                         aTCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGttt                                                                                                              <  2:691392/140‑1 (MQ=255)                                    gaaTAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGc                                                                                                   >  1:727408/1‑140 (MQ=255)                                      aTAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAg                                                                                                 <  2:550083/140‑1 (MQ=255)                                      aTAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAg                                                                                                 <  1:209682/140‑1 (MQ=255)                                        aaaCCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCt                                                                                               <  1:907259/140‑1 (MQ=255)                                             gctcgctGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACtt                                                                                          >  2:242522/1‑140 (MQ=255)                                                cgctGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACttttt                                                                                       >  1:461539/1‑140 (MQ=255)                                                      ccGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTttccttcc                                                                                 >  2:171239/1‑140 (MQ=255)                                                               ccGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGt                                                                        >  1:622538/1‑140 (MQ=255)                                                                         aaTTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGc                                                              <  1:65314/140‑1 (MQ=255)                                                                           ttAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGGCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGccc                                                            <  2:727408/140‑1 (MQ=255)                                                                                 ggTTGGCTTGCCAGAACTCAGCAAATGGTGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTcc                                                      >  2:100168/1‑140 (MQ=255)                                                                                   ttGGCTTGCCAGAACTAAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCa                                                    >  1:530487/1‑140 (MQ=255)                                                                                    tGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAg                                                   <  1:171239/140‑1 (MQ=255)                                                                                     ggCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTcc                                                      <  2:75895/136‑1 (MQ=255)                                                                                     ggCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTcc                                                      >  1:75895/1‑136 (MQ=255)                                                                                     ggCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGc                                                  >  1:220832/1‑140 (MQ=255)                                                                                       cTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGt                                                <  2:66336/140‑1 (MQ=255)                                                                                               aaCTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTaa                                        <  2:220832/140‑1 (MQ=255)                                                                                                aCTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAAc                                       <  2:37574/140‑1 (MQ=255)                                                                                                 cTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACt                                      <  2:873963/140‑1 (MQ=255)                                                                                                 cTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACt                                      <  2:622538/140‑1 (MQ=255)                                                                                                    aGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCg                                                 <  2:50869/126‑1 (MQ=255)                                                                                                    aGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCg                                                 >  1:285884/1‑126 (MQ=255)                                                                                                    aGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCg                                                 >  1:50869/1‑126 (MQ=255)                                                                                                    aGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCg                                                 <  2:285884/126‑1 (MQ=255)                                                                                                    aGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCgg                                   >  2:312760/1‑140 (MQ=255)                                                                                                    aGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCgg                                   >  2:79533/1‑140 (MQ=255)                                                                                                           ggcggcAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAAc                            >  1:243699/1‑140 (MQ=255)                                                                                                           ggcggcAATTCAACCTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAAc                            >  2:231485/1‑140 (MQ=255)                                                                                                                        ccTGCTGGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAACATCAAAATCATTa               <  1:312760/140‑1 (MQ=255)                                                                                                                          tgctgGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAACATCAAAATCATTAcg             <  2:563881/140‑1 (MQ=255)                                                                                                                            ctgGCTTGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAACATCAAAATCATTAcgcg           <  1:896877/140‑1 (MQ=255)                                                                                                                                 ttGCCCCTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAACATCAAAATCATTACGCGCTTCa      <  1:79533/140‑1 (MQ=255)                                                                                                                                    ccccTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAACATCAAAATCATTACGCGCTTCATAg   <  2:530487/140‑1 (MQ=255)                                                                                                                                    ccccTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAACATCAAAATCATTACGCGCTTCATAg   >  2:290095/1‑140 (MQ=255)                                                                                                                                     cccTCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAACATCAAAATCATTACGCGCTTCATAGc  <  1:598539/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         ATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAAACGTGCATAACCGCCCTTCTCCCAGCGTTCACTTAACTCGGCAAAAACATCAAAATCATTACGCGCTTCATAGC  >  NC_000913/3714622‑3714893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑