Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 763,390 C→A S126Y (TCC→TAC)  sucC → succinyl‑CoA synthetase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913763,3900CA97.1% 102.9 / ‑2.6 34S126Y (TCC→TAC) sucCsuccinyl‑CoA synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (18/15);  total (19/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

AACGTATCAAACAGATGCCAATGGCCAACCGGTTAACCAGATTCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGA  >  NC_000913/763259‑763522
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aaCGTATCAAACAGATGCCAATGGCCAACCGGTTAACCAGATTCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAg                                                                                                                              <  2:859898/140‑1 (MQ=255)
     aTCAAACAGATGCCAATGGCCAACCGGTTAACCAGATTCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAggcggc                                                                                                                         >  2:6789/1‑140 (MQ=255)
              aTGCCAATGGCCAACCGGTTAACCAGATTCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATc                                                                                                                >  2:625027/1‑140 (MQ=255)
                   aaTGGCCAACCGGTTAACCAGATTCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGaaaa                                                                                                           <  2:402210/140‑1 (MQ=255)
                        ccAACCGGTTAACCAGATTCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGGCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTgg                                                                                                      >  2:918134/1‑140 (MQ=255)
                            ccGGTTAACCAGATTCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGga                                                                                                  >  1:631562/1‑140 (MQ=255)
                                    ccAGATTCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTc                                                                                          <  1:625027/140‑1 (MQ=255)
                                          tCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCg                                                                                        <  1:462838/136‑1 (MQ=255)
                                          tCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCg                                                                                        >  2:462838/1‑136 (MQ=255)
                                          tCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCAcc                                                                                    >  1:113986/1‑140 (MQ=255)
                                          tCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCAcc                                                                                    >  1:495176/1‑140 (MQ=255)
                                            tGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTg                                                                                  <  2:113986/140‑1 (MQ=255)
                                            tGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTg                                                                                  <  1:6789/140‑1 (MQ=255)
                                              gTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGgaag                                                                                                <  2:180667/124‑1 (MQ=255)
                                              gTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGgaag                                                                                                >  1:180667/1‑124 (MQ=255)
                                                  aaGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCAt                                                                            <  1:217737/140‑1 (MQ=255)
                                                   agcagcGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATa                                                                           >  1:842833/1‑140 (MQ=255)
                                                                             tGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGc                                                 >  1:820518/1‑140 (MQ=255)
                                                                                  ctcGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAATCTCCGCTCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGAt                                            <  1:918134/140‑1 (MQ=255)
                                                                                      gTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCTATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCg                                        >  2:568980/1‑140 (MQ=255)
                                                                                      gTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATg                                           <  1:507799/137‑1 (MQ=255)
                                                                                      gTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATg                                           >  2:507799/1‑137 (MQ=255)
                                                                                      gTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCg                                        >  1:937460/1‑140 (MQ=255)
                                                                                                      tAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGc                        >  1:521513/1‑140 (MQ=255)
                                                                                                      tAGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGAACCATAAAGTTGGGCTTGATCCGCTGACTGGCCCGCTGCCGTATCAGGGACgcgcgc                        >  2:387224/1‑140 (MQ=255)
                                                                                                       aGTTCCCGTTGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCt                       <  1:766393/140‑1 (MQ=255)
                                                                                                       aGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCt                       >  1:292477/1‑140 (MQ=255)
                                                                                                       aGTTCCCGTCGTGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCt                       >  1:421799/1‑140 (MQ=255)
                                                                                                             cgtcgtGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCTATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGtt                 <  1:568980/140‑1 (MQ=255)
                                                                                                                cgtGTGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCaa              >  1:429077/1‑140 (MQ=255)
                                                                                                                 gtgtGGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCaaa             <  1:387224/140‑1 (MQ=255)
                                                                                                                   gtgGTCTTTATGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACt           >  1:515291/1‑140 (MQ=255)
                                                                                                                            aTGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGa  <  2:515291/140‑1 (MQ=255)
                                                                                                                            aTGGCCTACACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGa  <  2:951674/140‑1 (MQ=255)
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AACGTATCAAACAGATGCCAATGGCCAACCGGTTAACCAGATTCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGA  >  NC_000913/763259‑763522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑