Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4440TA30.3% 45.7 / 12.2 33intergenic (‑188/‑19)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (2/21);  new base A (10/0);  total (12/21)
Fisher's exact test for biased strand distribution p-value = 7.13e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAC  >  NC_000913/3206308‑3206577
                                                                                                                                          |                                                                                                                                      
cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACa                                                                                                                                     <  2:1223400/140‑1 (MQ=255)
   tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAc                                                                                                                                      >  1:186378/1‑136 (MQ=255)
   tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAc                                                                                                                                      <  2:186378/136‑1 (MQ=255)
    cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCAAAAAAAACaaaaa                                                                                                                                 >  2:166440/1‑140 (MQ=255)
    cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCCGAcaaaaacaaaaa                                                                                                                                 >  2:1175533/1‑140 (MQ=255)
       tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTT‑GATTTTTTCATAcaaaaacaaaaaaaa                                                                                                                              >  2:458361/1‑137 (MQ=255)
         ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGAcaaaaacaaaaaattga                                                                                                                            >  2:876945/1‑135 (MQ=255)
          tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                           <  2:567911/140‑1 (MQ=255)
             ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCCAAAAAAAAAAAAAAATGGAGTTg                                                                                                                        >  1:751120/1‑140 (MQ=255)
              gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGc                                                                                                                       <  2:1006738/140‑1 (MQ=255)
               cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTCTACAAAAAAAAAAAATTGAGTTGcc                                                                                                                      >  2:458699/1‑140 (MQ=255)
                   atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAAAGGAGTTGCCatga                                                                                                                  >  1:393846/1‑140 (MQ=255)
                       tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                              <  1:1175533/140‑1 (MQ=255)
                          ttcTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                           <  1:876945/140‑1 (MQ=255)
                                ttttCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaa                                                                                                     <  1:988878/140‑1 (MQ=255)
                                 tttCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                    <  1:471548/140‑1 (MQ=255)
                                     gcgTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGGAAGgaataaaa                                                                                                >  2:912306/1‑138 (MQ=255)
                                           aTTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                          <  1:239746/140‑1 (MQ=255)
                                             tCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                        <  1:458361/140‑1 (MQ=255)
                                                   aCTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AAt                                                                                 <  1:1126699/140‑1 (MQ=255)
                                                     gacagaTT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATaa                                                                               <  1:329101/135‑1 (MQ=255)
                                                        tAATTAACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCA‑ACAAAAAAAAAAAATGGAGTTTGCATGATGAGCGAAAATAAAAAACAAAAAGCAGGG‑Aaaaaat                                                                             >  2:351791/1‑138 (MQ=255)
                                                                atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑TATTTTTTCATACAAATAAAAAAAAAAGAGTTTTCCTGATGAGCGGAAGGAATAAACCAAAGGGAGGG‑AAAAAGATTTCagag                                                                     >  2:463288/1‑140 (MQ=255)
                                                                 tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAgaga                                                                    <  1:166440/140‑1 (MQ=255)
                                                                  atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGGGTTGCCATGATGAGCGAAAAGAAAAAAAAAAAAGCAGGG‑AATAAATTGACAgagaa                                                                   >  2:295853/1‑139 (MQ=255)
                                                                  atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAACAAAAAAAGGAGTTGGCATTATGAGGGAAAGAAATAAAAAAAAAGCAGTG‑AATAAATTGACAgagaa                                                                   >  2:1315982/1‑139 (MQ=255)
                                                                      aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCCTGATGAGGGAAAAGAAAAAAAAAAAAGCAGGG‑AAAAAAATGACAGAGACTGTc                                                               >  2:329101/1‑140 (MQ=255)
                                                                        ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAATAAAAAAAAAGGAGGTGCCCTGATGAGGGAAAGTAATAAACAACAAGCAGGG‑AATAAGTTGACAGAGATTGTTGc                                                             >  2:1133432/1‑140 (MQ=255)
                                                                          tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAAAAAAAAATGGAGTTGGCATGATGGGCGGAAAAAAAAAACAAAAGGCAGTG‑AATAAATTGACAGAGATTGTCGCTa                                                           >  2:1198350/1‑140 (MQ=255)
                                                                            gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGAGGTGGCATGATGGGCGAAAAAAAAAAACAAAAAGCAATG‑AATAAATTGAAAAAGAGTGTTGCTaaa                                                         >  2:1009296/1‑139 (MQ=255)
                                                                                     ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc                                                <  1:1315982/140‑1 (MQ=255)
                                                                                     acaaaaCCACATTTTTAGTGGGAGTTTGCGGCGTAG‑ATTTTTTCCAGCCAAAAACAAAACTTGGAGTTGCCGAGAAGACCGAAAGAAATAAGCAAGAGGCAGTA‑AATAGGTTGACAGAAATTGTCGAAAATTTTCCCGcc                                                <  2:374525/138‑1 (MQ=255)
                                                                                      taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGGGGTGGCCTGATGAGGGGAAAGAAAAAAAAACAAGCAGTGAAAAAAGTTG‑CAGAGATTTTTGGTAAATTTTCccccc                                               >  2:1308116/1‑139 (MQ=255)
                                                                                      aaaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                               <  1:1308116/139‑1 (MQ=255)
                                                                                               aTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAACAGGAGTTTGCCTGATGGGCGAAAGGAAAAAACAAAAAGCAGTG‑AATAAATTGACAGAGATTGTTGCTTACTTTAACCCCATGAtttttt                                      >  2:913562/1‑137 (MQ=255)
                                                                                                    tAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                 <  2:1205927/140‑1 (MQ=255)
                                                                                                                 gggCGGTA‑AATTTTCCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACg                    <  1:913562/139‑1 (MQ=255)
                                                                                                                         aaaTTTTTCCAGACAAATAAAAAAACGGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa             <  1:175444/139‑1 (MQ=255)
                                                                                                                            tttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa          <  1:351791/140‑1 (MQ=255)
                                                                                                                                 ccAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa     <  1:883032/140‑1 (MQ=255)
                                                                                                                                   aaaaaaaTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa   <  1:912306/136‑1 (MQ=255)
                                                                                                                                    gACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc  >  2:36152/1‑140 (MQ=255)
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CATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATT‑ACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAC  >  NC_000913/3206308‑3206577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑