Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,669	G→T	S103* (TCG→TAG)	rfbD ←	dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,669	0	G	T	100.0%	190.3 / NA	54	S103* (TCG→TAG)	rfbD	dTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/27);  total (27/27)

AATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGCATTGACAAT  >  NC_000913/2111533‑2111805                                                                                                                                         |                                                                                                                                         aaTAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGt                                                                                                                                       <  1:212682/140‑1 (MQ=255)  aTAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTa                                                                                                                                      <  2:816629/140‑1 (MQ=255)       aTGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCTGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGa                                                                                                                                 <  1:236312/140‑1 (MQ=255)            tCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAAcc                                                                                                                            <  1:88341/140‑1 (MQ=255)            tCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAAcc                                                                                                                            >  2:720702/1‑140 (MQ=255)            tCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAAcc                                                                                                                            >  2:329970/1‑140 (MQ=255)            tCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAAcc                                                                                                                            <  2:438615/140‑1 (MQ=255)                  aaTATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCt                                                                                                                      >  1:739615/1‑140 (MQ=255)                              aCGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCAttt                                                                                                          >  1:1332017/1‑140 (MQ=255)                              aCGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCAGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCAttt                                                                                                          >  2:781200/1‑140 (MQ=255)                                 cTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTtgct                                                                                                       >  1:233333/1‑140 (MQ=255)                                            gCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAAt                                                                                            <  1:864252/140‑1 (MQ=255)                                                aCTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCt                                                                                        <  2:219956/140‑1 (MQ=255)                                                          aCCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTc                                                                                      <  1:1058451/132‑1 (MQ=255)                                                          aCCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTc                                                                                      >  2:1058451/1‑132 (MQ=255)                                                          aCCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACttg                                                                              >  2:541913/1‑140 (MQ=255)                                                           ccGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACttgt                                                                             >  2:113067/1‑140 (MQ=255)                                                           ccGTAAACATTTAGTGGTGCGGTTGCATCAGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACttgt                                                                             <  1:781200/140‑1 (MQ=255)                                                                      tAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGttaatta                                                                  >  1:252229/1‑140 (MQ=255)                                                                      tAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGttaatta                                                                  >  2:267667/1‑140 (MQ=255)                                                                      tAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGttaatta                                                                  >  1:832303/1‑140 (MQ=255)                                                                        gtggtgCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTtaattaat                                                                <  1:247079/140‑1 (MQ=255)                                                                             gCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGc                                                           >  1:343466/1‑140 (MQ=255)                                                                             gCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGc                                                           <  2:739615/140‑1 (MQ=255)                                                                                   gCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTc                                                     <  2:1332017/140‑1 (MQ=255)                                                                                    cATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTcc                                                    <  1:267667/140‑1 (MQ=255)                                                                                      tCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCgg                                                  >  2:519878/1‑140 (MQ=255)                                                                                          tctcCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCt                                              >  2:141053/1‑140 (MQ=255)                                                                                           ctcCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTg                                             >  2:857247/1‑140 (MQ=255)                                                                                              cAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGAtt                                          <  2:233333/140‑1 (MQ=255)                                                                                                   aTGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCt                                     <  2:403738/140‑1 (MQ=255)                                                                                                   aTGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCt                                     <  2:955396/140‑1 (MQ=255)                                                                                                   aTGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCt                                     <  1:904553/140‑1 (MQ=255)                                                                                                     ggCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCttt                                   >  1:1165476/1‑140 (MQ=255)                                                                                                     ggCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCttt                                   <  1:857247/140‑1 (MQ=255)                                                                                                     ggCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCttt                                   >  1:12730/1‑140 (MQ=255)                                                                                                     ggCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCttt                                   <  2:330371/140‑1 (MQ=255)                                                                                                     ggCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCttt                                   >  1:330371/1‑140 (MQ=255)                                                                                                     ggCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCt                                     <  1:1311058/138‑1 (MQ=255)                                                                                                     ggCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCt                                     >  2:1311058/1‑138 (MQ=255)                                                                                                      gCATATCGCCATTTCCAGGGAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTg                                  <  1:113067/140‑1 (MQ=255)                                                                                                                        ggAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGt                                 <  2:20121/123‑1 (MQ=255)                                                                                                                        ggAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGt                                 >  1:20121/1‑123 (MQ=255)                                                                                                                         gAAGACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCgg               <  1:141053/140‑1 (MQ=255)                                                                                                                            gACGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTg            >  1:1002016/1‑140 (MQ=255)                                                                                                                             aCGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGc           >  1:301199/1‑140 (MQ=255)                                                                                                                             aCGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGc           >  2:296774/1‑140 (MQ=255)                                                                                                                             aCGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGc           >  2:175348/1‑140 (MQ=255)                                                                                                                              cGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGCa          >  1:869784/1‑140 (MQ=255)                                                                                                                              cGTAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGCa          <  2:832303/140‑1 (MQ=255)                                                                                                                                tAATCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGCAtt        <  2:1165476/140‑1 (MQ=255)                                                                                                                                  aTCAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGCATTGa      <  1:185504/140‑1 (MQ=255)                                                                                                                                    cAGTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGCATTGACa    >  1:498608/1‑140 (MQ=255)                                                                                                                                      gTCTAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGCATTGACaat  <  2:869784/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGGATAACCCAGGCTCCAACTTCATTTGCTGCTTTCGCAATCGCTTCGACACTTGTTGCGTTAATTAATTGTGCAAACTCCGGTTCTGATTCTGCTTTGTCTACTGCGGTGTGAGCGGCTGCATTGACAAT  >  NC_000913/2111533‑2111805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑