Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,436	(C)5→4	coding (1377/3099 nt)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,432	0	C	.	100.0%	252.8 / NA	60	coding (1373/3099 nt)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (24/36);  total (24/36)

ACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATAT  >  NC_000913/3017295‑3017564                                                                                                                                          |                                                                                                                                     aCTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGccc                                                                                                                                    <  1:1122955/140‑1 (MQ=255) aCTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGccc                                                                                                                                    <  2:847656/140‑1 (MQ=255) aCTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGccc                                                                                                                                    <  1:909364/140‑1 (MQ=255)     ggccATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGcccctgt                                                                                                                                >  1:210005/3‑137 (MQ=255)         aTGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTACCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTa                                                                                                                           <  1:502586/140‑1 (MQ=255)          tGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTAc                                                                                                                          >  1:54533/1‑140 (MQ=255)             tGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTActgc                                                                                                                       >  1:1189326/1‑140 (MQ=255)                cGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTActgcctg                                                                                                                    <  2:1142434/140‑1 (MQ=255)                  tCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTgcg                                                                                                                  <  2:1055784/140‑1 (MQ=255)                   cGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTgcgc                                                                                                                 <  1:951198/140‑1 (MQ=255)                     aaCGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTa                                                                                                               >  2:909070/1‑140 (MQ=255)                     aaCGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTa                                                                                                               <  1:909070/140‑1 (MQ=255)                     aaCGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTa                                                                                                               >  2:249523/1‑140 (MQ=255)                          cTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATcaag                                                                                                          <  1:1027482/140‑1 (MQ=255)                           tGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATcaagc                                                                                                         >  1:960248/1‑140 (MQ=255)                            gAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGTCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATcaagca                                                                                                        <  2:613820/140‑1 (MQ=255)                                  aCTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATcaag                                                                                                          <  2:1198679/132‑1 (MQ=255)                                  aCTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATcaag                                                                                                          >  1:1198679/1‑132 (MQ=255)                                  aCTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGatat                                                                                                  >  2:589889/1‑140 (MQ=255)                                  aCTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGatat                                                                                                  >  1:337870/1‑140 (MQ=255)                                  aCTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGatat                                                                                                  >  1:836602/1‑140 (MQ=255)                                    tGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTc                                                                                                >  2:969702/1‑140 (MQ=255)                                    tGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTc                                                                                                >  1:681898/1‑140 (MQ=255)                                    tGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTc                                                                                                <  1:620391/140‑1 (MQ=255)                                    tGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTc                                                                                                >  1:127347/1‑140 (MQ=255)                                    tGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTc                                                                                                >  1:352584/1‑140 (MQ=255)                                    tGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTc                                                                                                <  1:277695/140‑1 (MQ=255)                                        agcagATGCGTTAACCATGGAATACCCCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGa                                                                                            <  2:1222070/140‑1 (MQ=255)                                        agcagATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGa                                                                                            <  2:1199387/140‑1 (MQ=255)                                         gcagATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGaa                                                                                           <  1:1194334/140‑1 (MQ=255)                                           agATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATa                                                                                         <  2:836602/140‑1 (MQ=255)                                            gATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATAc                                                                                        >  2:106917/1‑140 (MQ=255)                                            gATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATAc                                                                                        >  1:226107/1‑140 (MQ=255)                                                  ttAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGt                                                                                  <  1:796233/140‑1 (MQ=255)                                                      ccATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGc                                                                              <  2:149887/140‑1 (MQ=255)                                                       cATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGc                                                                              <  2:249747/139‑1 (MQ=255)                                                       cATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGc                                                                              >  1:249747/1‑139 (MQ=255)                                                         tGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTg                                                                           <  2:127347/140‑1 (MQ=255)                                                         tGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTg                                                                           <  2:681898/140‑1 (MQ=255)                                                          ggAATACATCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTgg                                                                          >  1:1102178/1‑140 (MQ=255)                                                           gAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGc                                                                         <  2:252985/140‑1 (MQ=255)                                                              tACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGaa                                                                      <  2:428013/140‑1 (MQ=255)                                                               acacCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAac                                                                     >  1:881910/1‑140 (MQ=255)                                                                     aGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGaa                                                                      >  2:450866/1‑133 (MQ=255)                                                                     aGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGaa                                                                      <  1:450866/133‑1 (MQ=255)                                                                      gAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTa                                                              >  1:596383/1‑140 (MQ=255)                                                                            cTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGa                                                        <  1:1236014/140‑1 (MQ=255)                                                                            cTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGa                                                        <  2:395398/140‑1 (MQ=255)                                                                                    cAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTgg                                                <  2:1189326/140‑1 (MQ=255)                                                                                     agaagaGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGa                                               >  2:322232/1‑140 (MQ=255)                                                                                     agaagaGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGa                                               <  1:589889/140‑1 (MQ=255)                                                                                     agaagaGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGa                                               <  1:249523/140‑1 (MQ=255)                                                                                       aagaGCGAATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGAGCTGGAAc                                             >  2:845568/1‑140 (MQ=255)                                                                                              tATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTa                                      >  2:734406/1‑140 (MQ=255)                                                                                               aTTGAGGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTAc                                     <  2:210005/140‑1 (MQ=255)                                                                                               aTTGAAGTGGGAGAACACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTAc                                     <  2:688749/140‑1 (MQ=255)                                                                                               aTTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTAc                                     <  1:734406/140‑1 (MQ=255)                                                                                                 tGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACca                                   <  2:226107/140‑1 (MQ=255)                                                                                                 tGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACca                                   <  2:596383/140‑1 (MQ=255)                                                                                                      tGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACcaacgca                              >  1:1328835/1‑140 (MQ=255)                                                                                                      tGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACcaacgca                              <  1:322232/140‑1 (MQ=255)                                                                                                         cAGAAGACCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACcaacgcaacg                           <  1:1216044/140‑1 (MQ=255)                                                                                                           gaagaCCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGct                         <  2:54533/140‑1 (MQ=255)                                                                                                           gaagaCCTGCCGCTGACCGACTGCTACGTTG‑CCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGct                         <  1:106917/140‑1 (MQ=255)                                                                                                                                  cTAGGTTG‑CCCCTGTTTTACTGCCTGCGCTATCAAGAAAGATATCCCGAAATACACCCGTCTGCTTGGCGAACCCCGCTAGGCCGACCCGCTGGAACTCATCTCCCAACGAACCGCTCTGCCCGCCATTACCGGTCatat  <  2:352584/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     ACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATAT  >  NC_000913/3017295‑3017564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑