Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,114,775	(G)5→6	coding (716/1227 nt)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,114,770	1	.	G	98.2%	198.9 / ‑3.4	55	coding (721/1227 nt)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (27/27);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

GTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAAT‑GGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCATCTCTTTTTTGCTGACCGGCTGGAA  >  NC_000913/1114632‑1114902                                                                                                                                            |                                                                                                                                      gTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAAT‑g                                                                                                                                      >  1:886142/1‑140 (MQ=255)  tCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAAT‑gg                                                                                                                                     >  1:157782/1‑140 (MQ=255)      ttccGATCGCAAATTTGGCGGGGTGGTGGTCCATTTAGAAGCGCCGCCCCGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAAT‑ggg‑ggg                                                                                                                                <  2:268741/137‑2 (MQ=255)          gctCGCAAATTTTGCCGGTTCGGGGGGCCTTAAGAAGACCCCCCCCGCCTGCCACCGGGGCAATCATGCCACTGATAAAGGCGGCGTTACGGCCTTTACCCGCCAGTTCGCGGACATACAGCGTCAGAAT‑ggg‑gggcaat                                                                                                                            <  2:754025/138‑6 (MQ=255)           aTCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAAtt                                                                                                                            >  1:345666/1‑140 (MQ=255)                    tttGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTc                                                                                                                   >  2:1095381/1‑140 (MQ=255)                         agacaggtggtgCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCAc                                                                                                              <  1:643438/134‑1 (MQ=255)                             tCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTgg                                                                                                          >  1:1294519/1‑140 (MQ=255)                                      attaaGCAGACCCCCCCCCCCGGGCCCCGAGGCGATCATCCCACTGAAAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAAc                                                                                                 <  2:813749/136‑1 (MQ=255)                                             agagCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGa                                                                                          >  1:116787/1‑140 (MQ=255)                                               gcccgccACCCCGGGCCCCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTCCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACa                                                                                        <  2:1197833/138‑1 (MQ=255)                                               agCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACa                                                                                        >  2:660677/1‑140 (MQ=255)                                                 ccgccACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACaaa                                                                                      <  1:680079/140‑1 (MQ=255)                                                     cACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAgg                                                                                  <  1:660677/140‑1 (MQ=255)                                                        gCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTg                                                                               <  2:116787/140‑1 (MQ=255)                                                             gCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTAc                                                                          >  1:770591/1‑140 (MQ=255)                                                             gCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTAc                                                                          <  1:1190812/140‑1 (MQ=255)                                                             gCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTAc                                                                          >  2:766052/1‑140 (MQ=255)                                                                  gAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGtt                                                                     >  2:596954/1‑140 (MQ=255)                                                                   aGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGttt                                                                    >  2:450582/1‑140 (MQ=255)                                                                    gggaacaaTGCCACTAAAAAAGGCGACTTTACTGACTTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTc                                                                   <  2:402551/133‑1 (MQ=255)                                                                    ggCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTc                                                                   <  1:1095381/140‑1 (MQ=255)                                                                      cGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCgg                                                                 >  2:331746/1‑140 (MQ=255)                                                                        aTCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGt                                                               >  1:1091406/1‑140 (MQ=255)                                                                        aTCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGt                                                               >  2:1452430/1‑140 (MQ=255)                                                                             gCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTa                                                          >  1:450556/1‑140 (MQ=255)                                                                              ccACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTaa                                                         <  1:508436/140‑1 (MQ=255)                                                                                aCTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGt                                                       <  1:450582/140‑1 (MQ=255)                                                                                aCTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGt                                                       <  1:164542/140‑1 (MQ=255)                                                                                  tGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGa                                                     >  2:427608/1‑140 (MQ=255)                                                                                  tGAAAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGa                                                     <  2:519180/140‑1 (MQ=255)                                                                                    aTAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATg                                                   >  2:501977/1‑140 (MQ=255)                                                                                     aaaaGGCGACGTTACTGACTTTCCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGt                                                  <  2:1328645/139‑1 (MQ=255)                                                                                        aGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTcac                                               <  1:154500/140‑1 (MQ=255)                                                                                        aGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTcac                                               <  1:427608/140‑1 (MQ=255)                                                                                          gggACGTTACTGCCGTTCCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTcacca                                             <  2:886142/138‑1 (MQ=255)                                                                                             aCGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACtt                                          >  1:815205/1‑140 (MQ=255)                                                                                               gTTACTGACGTTCCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTcc                                        <  1:596954/140‑1 (MQ=255)                                                                                               gTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTcc                                        <  1:1452430/140‑1 (MQ=255)                                                                                               gTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTcc                                        <  2:46451/140‑1 (MQ=255)                                                                                                  aCTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGc                                     <  1:397215/140‑1 (MQ=255)                                                                                                  aCTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGc                                     <  2:798095/140‑1 (MQ=255)                                                                                                    tGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCAt                                   >  2:839189/1‑140 (MQ=255)                                                                                                       cGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCAtgtg                                <  1:766052/140‑1 (MQ=255)                                                                                                       cGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCAtgtg                                <  2:1091406/140‑1 (MQ=255)                                                                                                        gTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgc                               <  2:815205/140‑1 (MQ=255)                                                                                                           aCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagc                            >  1:329689/1‑140 (MQ=255)                                                                                                           aCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagc                            >  2:615174/1‑140 (MQ=255)                                                                                                           aCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagc                            >  2:707851/1‑140 (MQ=255)                                                                                                           aCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagc                            >  1:1168473/1‑140 (MQ=255)                                                                                                           aCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagc                            >  2:1164084/1‑140 (MQ=255)                                                                                                           aCCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagc                            >  1:85139/1‑140 (MQ=255)                                                                                                           aCCCGCCAGTTCGCGGACATACAGCGTCAGAAT‑GGGTGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagc                            >  2:396326/1‑140 (MQ=255)                                                                                                            cccGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagca                           <  2:329689/140‑1 (MQ=255)                                                                                                             ccGCCAGTTCGCGGACATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCGCTTCCCGCATGTGCAGCAt                          >  2:1102169/1‑140 (MQ=255)                                                                                                                           aCATACAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCATCTCTTTTTTGCTGa            <  1:136797/140‑1 (MQ=255)                                                                                                                               aCAGCGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCATCTCTTTTTTGCTGACCgg        >  1:1392639/1‑140 (MQ=255)                                                                                                                                   cGTCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCATCTCTTTTTTGCTGACCGGCTgg    >  2:708187/1‑140 (MQ=255)                                                                                                                                     tCAGAATGGGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCATCTCTTTTTTGCTGACCGGCTGGaa  >  2:12508/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      GTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAAT‑GGG‑GGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCATCTCTTTTTTGCTGACCGGCTGGAA  >  NC_000913/1114632‑1114902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑