| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 372,640 | 0 | T | G | 28.9% | 83.2 / 11.7 | 45 | L176V (TTA→GTA) | mhpD | 2‑hydroxypentadienoate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (10/22); new base G (13/0); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAAC > NC_000913/372501‑372775 | cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCCACGTCCGGCGGGGt > 1:373188/1‑140 (MQ=255)cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGt < 1:1242533/140‑1 (MQ=255)cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGt > 2:1395101/1‑140 (MQ=255) ttGAATGGGAACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTa > 1:1204406/1‑140 (MQ=255) tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAg < 1:1446119/140‑1 (MQ=255) ggTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGa > 2:1387772/1‑140 (MQ=255) gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGaa > 1:1435750/1‑140 (MQ=255) gTACTTCCGGCACTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGGCCGGCGCAACGTCCGGCGGGGGTAGACCTGaa > 1:1347968/1‑140 (MQ=255) aCTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGaaaa > 1:223019/1‑140 (MQ=255) ccGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTg > 1:1016021/1‑140 (MQ=255) ggCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTgcg < 1:1395101/140‑1 (MQ=255) gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTgcgc > 1:928558/1‑140 (MQ=255) cgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGcc > 1:108590/1‑140 (MQ=255) ggAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAAATGCGCCCTGa > 2:64728/1‑140 (MQ=255) ggAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGGGTATGTCATCGGCGGGCCGGCCCAACGTCCGGCGGGGTTAGACCTGGAAAACAGCGCCATGa > 1:775877/1‑140 (MQ=255) tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcg < 2:1204406/140‑1 (MQ=255) gAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGta < 1:9355/140‑1 (MQ=255) aGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAAATGCGCCATGAAGATGACGCGgaa > 2:34721/1‑140 (MQ=255) aGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGGGTATGTCATCGGCGGTCCGGCGCAACGTTCGGCGGGGGTAGACCTGAAAAACTGCGCCATTAAGATGACCCGcaa > 2:1020555/1‑140 (MQ=255) ccGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAAATGCGGCATGAAGATGACGCGgaata > 2:544674/1‑140 (MQ=255) ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa < 2:161280/140‑1 (MQ=255) ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa < 2:373188/140‑1 (MQ=255) ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa < 1:1387772/140‑1 (MQ=255) gcgACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAagag < 2:619360/140‑1 (MQ=255) gACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAagag < 1:503704/138‑1 (MQ=255) gACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAagag > 2:503704/1‑138 (MQ=255) ggTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCt > 1:1397681/1‑140 (MQ=255) ggTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAAATGCGCCATGAAGATGACGCGGAATAAAGAAGAGGGTTCt > 2:266890/1‑140 (MQ=255) ttCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCggg > 1:977105/1‑140 (MQ=255) cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg < 2:1133505/140‑1 (MQ=255) cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCg > 1:431193/1‑140 (MQ=255) gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGACATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa < 2:170098/140‑1 (MQ=255) ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTgg < 2:1397681/140‑1 (MQ=255) aTAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACAt > 1:864627/1‑140 (MQ=255) cctGTGGGGTGTATGTCATCGGCGGTCCCGCGCCACGTCCCGCGGGGTTGGGACTGAAAAAATGCGCCCTGAAGATTGCGCCGAATAACGAAGAGGGTTCTCGCGGGGGCAGCCGCCAACGTCCCGGACATcctccttat > 2:677763/1‑133 (MQ=255) gtgGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg < 1:1020555/140‑1 (MQ=255) gggTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCCTGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGcc > 2:141204/1‑140 (MQ=255) tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg < 2:206863/132‑1 (MQ=255) tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAAAGCCTGGGACATCCGCTTAATGCg > 1:206863/1‑132 (MQ=255) tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCCTGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg > 1:1416121/1‑140 (MQ=255) aTGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctgg < 2:618221/140‑1 (MQ=255) cggcggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg > 2:605998/1‑124 (MQ=255) cggcggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg < 1:605998/124‑1 (MQ=255) cggcggTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc > 1:558026/1‑140 (MQ=255) cggcggTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc > 2:300957/1‑140 (MQ=255) cggcggTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAAATGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTTTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc > 2:1076225/1‑140 (MQ=255) gcggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGc > 2:997115/1‑140 (MQ=255) gcggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGc > 1:836242/1‑140 (MQ=255) ggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaa < 1:544674/140‑1 (MQ=255) tCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaaaa > 1:1113572/1‑140 (MQ=255) tCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaaaa > 2:117430/1‑140 (MQ=255) ggCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATgg < 2:288044/140‑1 (MQ=255) gcgcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGc < 2:108590/140‑1 (MQ=255) gcgcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGc < 1:997115/140‑1 (MQ=255) gcggGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGa > 2:1417083/1‑140 (MQ=255) cggGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGaa < 1:117430/140‑1 (MQ=255) ggggTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAAc < 2:392522/140‑1 (MQ=255) ttAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAAc > 1:730856/1‑136 (MQ=255) ttAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAAc < 2:730856/136‑1 (MQ=255) | CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAAC > NC_000913/372501‑372775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |