BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	153,654	A→G	R591R (CGT→CGC)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	153,654	0	A	G	100.0%	520.5 / NA	138	R591R (CGT→CGC)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (67/71); total (67/71)

Only 100 of 138 total aligned reads displayed.

TTATTCATCGTATAGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGTACTGTTACTGTA  >  NC_000913/153517‑153788

ttATTCATCGTATAGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCg                                                                                                                                      <  1:147017/140‑1 (MQ=255)

ttATTCATCGTATAGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCg                                                                                                                                      <  1:1409562/140‑1 (MQ=255)

ttATTCATCGTATAGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCg                                                                                                                                      <  2:703440/140‑1 (MQ=255)

       tCGTATAGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCa                                                                                                                               <  2:525631/140‑1 (MQ=255)

       tCGTATAGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCa                                                                                                                               >  2:757007/1‑140 (MQ=255)

       tCGTATAGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCa                                                                                                                               >  1:77720/1‑140 (MQ=255)

             aGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCa                                                                                                                         >  2:262510/1‑140 (MQ=255)

             aGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCa                                                                                                                         <  2:959593/140‑1 (MQ=255)

             aGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCa                                                                                                                         <  2:406732/140‑1 (MQ=255)

                  gTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTaa                                                                                                                    <  2:882009/140‑1 (MQ=255)

                  gTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTaa                                                                                                                    >  1:1147939/1‑140 (MQ=255)

                  gTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTaa                                                                                                                    >  2:360516/1‑140 (MQ=255)

                   tATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAAt                                                                                                                   <  1:47004/140‑1 (MQ=255)

                        aCGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTc                                                                                                              >  2:125884/1‑140 (MQ=255)

                          gCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCaa                                                                                                            >  2:1299438/1‑140 (MQ=255)

                          gCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCaa                                                                                                            <  1:125884/140‑1 (MQ=255)

                           cTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAAt                                                                                                           <  2:168918/140‑1 (MQ=255)

                             ataACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTg                                                                                                         >  1:42079/1‑140 (MQ=255)

                             ataACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTg                                                                                                         >  1:599485/1‑140 (MQ=255)

                               aaCTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGa                                                                                                       >  2:710855/1‑140 (MQ=255)

                                    aCGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGaa                                                                                                      >  1:1335299/1‑136 (MQ=255)

                                    aCGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATg                                                                                                    >  1:559305/1‑138 (MQ=255)

                                    aCGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGt                                                                                                  >  1:484959/1‑140 (MQ=255)

                                    aCGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGt                                                                                                  <  2:599485/140‑1 (MQ=255)

                                    aCGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGt                                                                                                  >  2:1285254/1‑140 (MQ=255)

                                    aCGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGt                                                                                                  >  1:1192958/1‑140 (MQ=255)

                                    aCGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGt                                                                                                  >  2:1009371/1‑140 (MQ=255)

                                    aCGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTCCCAAGTAATTTTTCAATTGGaa                                                                                                      <  2:1335299/136‑1 (MQ=255)

                                      gcgAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGa                                                                                                >  1:80257/1‑140 (MQ=255)

                                      gcgAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGa                                                                                                >  2:324892/1‑140 (MQ=255)

                                      gcgAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGa                                                                                                >  1:627011/1‑140 (MQ=255)

                                         aGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAAc                                                                                             >  1:567395/1‑140 (MQ=255)

                                                      taGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCt                                                                                <  1:1299438/140‑1 (MQ=255)

                                                           aCTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGt                                                                           <  1:369226/140‑1 (MQ=255)

                                                             tgctgcTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGt                                                                           <  2:76437/138‑1 (MQ=255)

                                                                tgctAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAgtg                                                                      >  2:266305/1‑140 (MQ=255)

                                                                    aaCGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGc                                                                  <  1:1238753/140‑1 (MQ=255)

                                                                      cGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCg                                                                <  1:848152/140‑1 (MQ=255)

                                                                       gTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGt                                                               >  1:1027739/1‑140 (MQ=255)

                                                                        ttGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTc                                                              <  2:675636/140‑1 (MQ=255)

                                                                         tGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCt                                                             >  1:694566/1‑140 (MQ=255)

                                                                          gAGTTGGTTATTACCTTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCtt                                                            >  2:647514/1‑140 (MQ=255)

                                                                          gAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCtt                                                            >  1:948496/1‑140 (MQ=255)

                                                                           aGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACCAAGATAAACCCTAACGTCAGTGTCGCCGTCTTc                                                           >  2:154183/1‑140 (MQ=255)

                                                                             ttGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCAt                                                         <  1:710855/140‑1 (MQ=255)

                                                                                  tattaCCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCAt                                                    <  2:567395/140‑1 (MQ=255)

                                                                                     taCCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGaa                                                 >  2:363346/1‑140 (MQ=255)

                                                                                       cccTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACg                                               >  1:1265079/1‑140 (MQ=255)

                                                                                        ccTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGc                                              <  2:401918/140‑1 (MQ=255)

                                                                                          ttAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTg                                            <  2:484959/140‑1 (MQ=255)

                                                                                          ttAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTg                                            <  2:597262/140‑1 (MQ=255)

                                                                                          ttAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTg                                            <  2:984198/140‑1 (MQ=255)

                                                                                          ttAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTg                                            >  2:1182783/1‑140 (MQ=255)

                                                                                          ttAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTg                                            >  1:367841/1‑140 (MQ=255)

                                                                                           tAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTgg                                           <  2:649145/140‑1 (MQ=255)

                                                                                            aaaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTggg                                          <  2:948496/140‑1 (MQ=255)

                                                                                            aaaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTggg                                          <  2:627011/140‑1 (MQ=255)

                                                                                             aaGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGc                                         <  2:1147939/140‑1 (MQ=255)

                                                                                               gTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCAc                                       <  1:754515/140‑1 (MQ=255)

                                                                                               gTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCAc                                       <  1:266305/140‑1 (MQ=255)

                                                                                                tCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACt                                      <  1:607709/140‑1 (MQ=255)

                                                                                                  aCTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCAc                                       <  1:223341/137‑1 (MQ=255)

                                                                                                  aCTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGa                                    <  1:136857/140‑1 (MQ=255)

                                                                                                  aCTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGa                                    >  1:244560/1‑140 (MQ=255)

                                                                                                          tttGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAg                            <  2:492472/140‑1 (MQ=255)

                                                                                                          tttGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAg                            <  1:363346/140‑1 (MQ=255)

                                                                                                          tttGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAg                            <  2:244560/140‑1 (MQ=255)

                                                                                                           ttGAGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGc                           <  1:647514/140‑1 (MQ=255)

                                                                                                              aGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGc                        >  1:799862/1‑140 (MQ=255)

                                                                                                              aGTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGc                        >  2:1407434/1‑140 (MQ=255)

                                                                                                               gTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGcc                       <  2:981563/140‑1 (MQ=255)

                                                                                                               gTATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGcc                       <  1:324892/140‑1 (MQ=255)

                                                                                                                tATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGccc                      >  1:998220/1‑140 (MQ=255)

                                                                                                                tATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGccc                      >  2:863861/1‑140 (MQ=255)

                                                                                                                tATCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGccc                      >  1:113644/1‑140 (MQ=255)

                                                                                                                 aTCAATACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGcccc                     >  1:318818/1‑140 (MQ=255)

                                                                                                                    aaTACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAgg                  <  1:1401612/140‑1 (MQ=255)

                                                                                                                    aaTACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAgg                  <  2:1280174/140‑1 (MQ=255)

                                                                                                                     aTACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGa                 >  2:559224/1‑140 (MQ=255)

                                                                                                                     aTACTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGa                 >  2:960958/1‑140 (MQ=255)

                                                                                                                       aCTCTGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATg               <  1:49708/140‑1 (MQ=255)

                                                                                                                        ctctGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGt              <  1:1285254/140‑1 (MQ=255)

                                                                                                                        ctctGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGt              <  2:624032/140‑1 (MQ=255)

                                                                                                                        ctctGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGt              >  2:1416944/1‑140 (MQ=255)

                                                                                                                         tctGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGta             <  1:559224/140‑1 (MQ=255)

                                                                                                                         tctGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGta             >  1:682683/1‑140 (MQ=255)

                                                                                                                          ctGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAgg                  >  1:647673/1‑134 (MQ=255)

                                                                                                                           tGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAgg                  <  2:1125927/133‑1 (MQ=255)

                                                                                                                           tGGAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAgg                  >  1:1125927/1‑133 (MQ=255)

                                                                                                                            ggAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGtactg          <  1:757007/140‑1 (MQ=255)

                                                                                                                             gAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGcc                       >  1:1412948/1‑126 (MQ=255)

                                                                                                                             gAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGcc                       <  2:1412948/126‑1 (MQ=255)

                                                                                                                             gAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGtactgt         >  2:847366/1‑140 (MQ=255)

                                                                                                                             gAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGtactgt         >  2:464991/1‑140 (MQ=255)

                                                                                                                             gAAACCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGtactgt         >  1:1076735/1‑140 (MQ=255)

                                                                                                                                aCCTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGtactgttac      >  1:1190117/1‑140 (MQ=255)

                                                                                                                                 ccTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGtactgttact     <  1:440316/140‑1 (MQ=255)

                                                                                                                                 ccTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGtactgttact     <  2:1027739/140‑1 (MQ=255)

                                                                                                                                 ccTGAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGtactgttact     <  2:694566/140‑1 (MQ=255)

                                                                                                                                    gAAGTGCGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGTACTGTTACTGTa  <  1:1009371/140‑1 (MQ=255)

TTATTCATCGTATAGCCAGTATTCACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCTGCCCCAGGATGTACTGTTACTGTA  >  NC_000913/153517‑153788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`