Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	823,547	C→T	A65T (GCG→ACG)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	823,547	0	C	T	98.6%	235.2 / ‑4.2	70	A65T (GCG→ACG)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (35/34);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

GTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGCTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACCGCGGGATAA  >  NC_000913/823423‑823680                                                                                                                             |                                                                                                                                      gTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTg                                                                                                                        >  2:742397/1‑140 (MQ=255)    cgcATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTgcgc                                                                                                                     >  2:918423/1‑140 (MQ=255)    cgcATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTgcgc                                                                                                                     >  2:280874/1‑140 (MQ=255)     gcATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgct                                                                                                                    <  2:505684/140‑1 (MQ=255)     gcATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgct                                                                                                                    <  2:1384209/140‑1 (MQ=255)     gcATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgct                                                                                                                    <  1:970681/140‑1 (MQ=255)     gcATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCAGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgct                                                                                                                    <  1:981461/140‑1 (MQ=255)      cATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgctg                                                                                                                   >  2:842740/1‑140 (MQ=255)       aTACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgctgc                                                                                                                  >  2:890297/1‑140 (MQ=255)        tACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgctgct                                                                                                                 <  1:1365185/140‑1 (MQ=255)        tACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgctgct                                                                                                                 <  1:919955/140‑1 (MQ=255)         aCCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGctgctg                                                                                                                >  2:1098708/1‑140 (MQ=255)               aaGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCagta                                                                                                          <  1:815287/140‑1 (MQ=255)                   cGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgct                                                                                                                    <  2:935328/126‑1 (MQ=255)                   cGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCgct                                                                                                                    >  1:935328/1‑126 (MQ=255)                   cGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGc                                                                                                      >  1:1010370/1‑140 (MQ=255)                    gAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGcc                                                                                                     <  1:280874/140‑1 (MQ=255)                    gAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCATAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGcc                                                                                                     <  2:1010370/140‑1 (MQ=255)                     aGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCg                                                                                                    >  2:885670/1‑140 (MQ=255)                     aGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCg                                                                                                    <  2:611425/140‑1 (MQ=255)                         cGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATg                                                                                                <  1:890297/140‑1 (MQ=255)                                      agCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCg                                                                                   >  1:306412/1‑140 (MQ=255)                                       gCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGa                                                                                  >  1:300308/1‑140 (MQ=255)                                       gCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGa                                                                                  >  2:316873/1‑140 (MQ=255)                                        cGGGACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGTTCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCGGTAGTGCCGCATGCAGTTGTTTGCCGAc                                                                                 >  2:713268/1‑140 (MQ=255)                                        cGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGAc                                                                                 >  2:564552/1‑140 (MQ=255)                                          gAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTTATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGt                                                                               <  1:1363311/140‑1 (MQ=255)                                          gAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGt                                                                               >  2:322794/1‑140 (MQ=255)                                           aaCACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTc                                                                              <  1:316873/140‑1 (MQ=255)                                           aaCACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTc                                                                              <  1:918423/140‑1 (MQ=255)                                           aaCACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTc                                                                              <  2:300308/140‑1 (MQ=255)                                            acacTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCa                                                                             >  1:1291761/1‑140 (MQ=255)                                             cacTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCAt                                                                            >  2:470839/1‑140 (MQ=255)                                             cacTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCAt                                                                            >  1:151686/1‑140 (MQ=255)                                                tACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCt                                                                         >  2:1006860/1‑140 (MQ=255)                                                 aCGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTc                                                                        >  2:1329219/1‑140 (MQ=255)                                                   gCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCaa                                                                      >  2:634568/1‑140 (MQ=255)                                                   gCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCaa                                                                      <  1:325377/140‑1 (MQ=255)                                                    ccAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCaaa                                                                     <  1:564552/140‑1 (MQ=255)                                                           ccGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATa                                                              <  2:1291761/140‑1 (MQ=255)                                                                  ttCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGttt                                                       >  1:1454385/1‑140 (MQ=255)                                                                        gACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGaa                                                 <  2:509521/140‑1 (MQ=255)                                                                          cAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAatg                                               <  1:210940/140‑1 (MQ=255)                                                                          cAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAatg                                               >  1:865874/1‑140 (MQ=255)                                                                            aaCTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATaaa                                                            >  1:357789/1‑125 (MQ=255)                                                                            aaCTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATaaa                                                            <  2:357789/125‑1 (MQ=255)                                                                            aaCTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAatgat                                             >  2:334401/1‑140 (MQ=255)                                                                              cacATCGCTGAGATCCGGTGTATCGTAGCCATCCAGCAAGACTTCCGTTTTATCCCCGCGTTGCGCTGCTGCCAGTAGTGCCTCATGCAGTTGTTTGCCGACGTGATCCTCAAACCAGATAAACGTTTCAAGAATGATGc                                           <  2:495965/138‑1 (MQ=255)                                                                               tCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCg                                          <  1:470839/140‑1 (MQ=255)                                                                                 aTCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGtt                                        >  2:922370/1‑140 (MQ=255)                                                                                        agaTCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGc                                 >  2:1252217/1‑140 (MQ=255)                                                                                         gaTCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGcc                                >  1:391582/1‑140 (MQ=255)                                                                                                cGACCCGTAGCCATCCAGCAAGTCTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTCTCAAGAATGATGCGTTCTTGTGCCTCGCCaa                         <  2:278207/140‑1 (MQ=255)                                                                                                cGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCaa                         <  1:322794/140‑1 (MQ=255)                                                                                                cGAACCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCaa                         <  2:865874/140‑1 (MQ=255)                                                                                                  aaCCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATc                       <  1:69592/140‑1 (MQ=255)                                                                                                  aaCCGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATc                       >  2:889867/1‑140 (MQ=255)                                                                                                    ccGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGc                     >  1:1347086/1‑140 (MQ=255)                                                                                                    ccGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGCTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGc                     >  2:874442/1‑140 (MQ=255)                                                                                                     cGTAGCCATCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGcc                    >  1:121732/1‑140 (MQ=255)                                                                                                             tCCAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAacac            <  2:391582/140‑1 (MQ=255)                                                                                                              ccAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACAcc           >  1:311967/1‑140 (MQ=255)                                                                                                              ccAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACAcc           <  1:742397/140‑1 (MQ=255)                                                                                                               cAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACcg          >  2:1280501/1‑140 (MQ=255)                                                                                                               cAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACcg          <  2:121732/140‑1 (MQ=255)                                                                                                               cAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACcg          <  1:1252217/140‑1 (MQ=255)                                                                                                               cAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACcg          <  1:1377827/140‑1 (MQ=255)                                                                                                               cAGCAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACcg          <  1:753069/140‑1 (MQ=255)                                                                                                                  cAAGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACCGCgg       >  1:85579/1‑140 (MQ=255)                                                                                                                   aaGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACCGCggg      >  2:237967/1‑140 (MQ=255)                                                                                                                    aGACTTCCGTTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACCGCGGGa     <  2:1454385/140‑1 (MQ=255)                                                                                                                       cTTCCGCTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACCGCGGGataa  >  2:1122442/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                      GTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGCTTTAACCCCGCGTTGCGCTGCTGCCAGTAGTGCCGCATGCAGTTGTTTGCCGACGTCATCCTCAAACCAGATAAACGTTTCAAGAATGATGCGTTCTTGTGCCTCGCCAATCGCCTTAAACACCGCGGGATAA  >  NC_000913/823423‑823680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑