Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	956,698	C→T	intergenic (‑66/‑64)	ycaO ← / → ycaP	ribosomal protein S12 methylthiotransferase accessory factor YcaO/DUF421 domain‑containing protein YcaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	956,698	0	C	T	100.0%	291.1 / NA	83	intergenic (‑66/‑64)	ycaO/ycaP	ribosomal protein S12 methylthiotransferase accessory factor YcaO/DUF421 domain‑containing protein YcaP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/49);  total (34/49)

TTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACCGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCA  >  NC_000913/956566‑956830                                                                                                                                     |                                                                                                                                     tttGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCa                                                                                                                               <  1:509063/140‑1 (MQ=255) tttGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCa                                                                                                                               <  1:205842/140‑1 (MQ=255)        gAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGAt                                                                                                                        <  1:591304/140‑1 (MQ=255)           gcgagcgaTGGAATCTTCCAGAGCGTCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGaa                                                                                                                     <  1:502825/140‑1 (MQ=255)            cgagcgaTGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAAc                                                                                                                    <  1:1160008/140‑1 (MQ=255)             gagcgaTGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACt                                                                                                                   <  2:751792/140‑1 (MQ=255)             gagcgaTGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACt                                                                                                                   <  2:541654/140‑1 (MQ=255)                 gaTGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTg                                                                                                               <  2:545323/140‑1 (MQ=255)                        tCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGc                                                                                                        <  1:1136266/140‑1 (MQ=255)                             cAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATTCGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTa                                                                                                   <  2:860104/140‑1 (MQ=255)                               gagCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGc                                                                                                 >  1:228692/1‑140 (MQ=255)                                 gCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCgg                                                                                               <  2:402283/140‑1 (MQ=255)                                   ggCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGAt                                                                                             <  1:250266/140‑1 (MQ=255)                                    gCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATa                                                                                            >  1:914141/1‑140 (MQ=255)                                     cATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATaa                                                                                           >  1:1269513/1‑140 (MQ=255)                                       tCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAAc                                                                                         <  1:95136/140‑1 (MQ=255)                                       tCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAAc                                                                                         <  2:713780/140‑1 (MQ=255)                                           tGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACg                                                                                     >  2:1396249/1‑140 (MQ=255)                                               ggggATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTaa                                                                                 <  2:228692/140‑1 (MQ=255)                                               ggggATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTaa                                                                                 <  2:1104705/140‑1 (MQ=255)                                                 ggATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAaga                                                                               >  2:442939/1‑140 (MQ=255)                                                           ttGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAAt                                                                     >  2:1325628/1‑140 (MQ=255)                                                            tGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATg                                                                    >  1:688987/1‑140 (MQ=255)                                                            tGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATg                                                                    >  2:188904/1‑140 (MQ=255)                                                            tGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATg                                                                    >  2:1293860/1‑140 (MQ=255)                                                            tGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATg                                                                    >  2:877837/1‑140 (MQ=255)                                                            tGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATg                                                                    >  2:290544/1‑140 (MQ=255)                                                              cGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGaa                                                                  <  1:1293860/140‑1 (MQ=255)                                                               gTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAg                                                                 <  2:914141/140‑1 (MQ=255)                                                               gTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAg                                                                 >  1:1264757/1‑140 (MQ=255)                                                                   tGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGt                                                             >  2:634304/1‑140 (MQ=255)                                                                    gAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGtt                                                            <  1:351036/140‑1 (MQ=255)                                                                    gAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGtt                                                            <  1:666446/140‑1 (MQ=255)                                                                    gAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCACTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGtt                                                            <  1:442939/140‑1 (MQ=255)                                                                        aTCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGAt                                                        <  1:290544/140‑1 (MQ=255)                                                                         tCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGAtc                                                       <  1:1204525/140‑1 (MQ=255)                                                                          cACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGAtct                                                      <  2:390763/140‑1 (MQ=255)                                                                           aCTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGAtctc                                                     <  2:1403622/140‑1 (MQ=255)                                                                           aCTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGAtctc                                                     <  2:688987/140‑1 (MQ=255)                                                                             tttAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCa                                                   <  1:188904/140‑1 (MQ=255)                                                                             tttAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCa                                                   <  1:634304/140‑1 (MQ=255)                                                                             tttAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCa                                                   <  1:144412/140‑1 (MQ=255)                                                                             tttAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGCCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCa                                                   <  2:389912/140‑1 (MQ=255)                                                                               tAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCAcc                                                 >  1:1079634/1‑140 (MQ=255)                                                                               tAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCAcc                                                 >  1:361049/1‑140 (MQ=255)                                                                                aGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCg                                                >  2:1366592/1‑140 (MQ=255)                                                                                  tCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTa                                              <  2:361049/140‑1 (MQ=255)                                                                                     tACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGtt                                                            >  1:1795/1‑123 (MQ=255)                                                                                     tACGGAAAGAGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGtt                                                            <  2:1795/123‑1 (MQ=255)                                                                                      aCGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGc                                          <  1:1427958/140‑1 (MQ=255)                                                                                       cGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCa                                         >  1:1263374/1‑140 (MQ=255)                                                                                        ggAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCAt                                        <  1:1092162/140‑1 (MQ=255)                                                                                         gAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCAtt                                       <  1:1396249/140‑1 (MQ=255)                                                                                                cgcAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATaaa                                >  1:17098/1‑140 (MQ=255)                                                                                                cgcAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATaaa                                >  1:1281441/1‑140 (MQ=255)                                                                                                cgcAATAATACGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATATGATaaa                                >  2:776859/1‑140 (MQ=255)                                                                                                    ataataCGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGc                            <  1:610248/140‑1 (MQ=255)                                                                                                     taataCGGGTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGcc                           <  1:215827/140‑1 (MQ=255)                                                                                                            ggTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATATGATAAAGTGCCTTTTGAt                    <  1:776859/140‑1 (MQ=255)                                                                                                             gTTTTATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGAtt                   >  2:661877/1‑140 (MQ=255)                                                                                                               tttATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTc                 >  2:1010124/1‑140 (MQ=255)                                                                                                               tttATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTc                 >  2:247725/1‑140 (MQ=255)                                                                                                                ttATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTcc                >  2:226037/1‑140 (MQ=255)                                                                                                                ttATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTcc                >  2:1008169/1‑140 (MQ=255)                                                                                                                 tATCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCt               >  2:1104868/1‑140 (MQ=255)                                                                                                                  aTCTCAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCtt              >  1:782975/1‑140 (MQ=255)                                                                                                                    ctcAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTgg            <  2:782975/140‑1 (MQ=255)                                                                                                                     tcAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGc           >  1:1394482/1‑140 (MQ=255)                                                                                                                      cAAAGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCg          <  2:1079634/140‑1 (MQ=255)                                                                                                                       aaaGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTc                 <  1:474561/132‑1 (MQ=255)                                                                                                                       aaaGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTc                 >  2:474561/1‑132 (MQ=255)                                                                                                                       aaaGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGa         >  2:666140/1‑140 (MQ=255)                                                                                                                       aaaGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGa         >  1:1114420/1‑140 (MQ=255)                                                                                                                       aaaGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGa         >  1:162871/1‑140 (MQ=255)                                                                                                                         aGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAg       <  2:1281441/140‑1 (MQ=255)                                                                                                                         aGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAg       <  1:1104868/140‑1 (MQ=255)                                                                                                                         aGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAg       <  2:17098/140‑1 (MQ=255)                                                                                                                         aGGCGCTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAg       <  2:925921/140‑1 (MQ=255)                                                                                                                             gcTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGc   <  2:639579/140‑1 (MQ=255)                                                                                                                             gcTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGc   <  2:162871/140‑1 (MQ=255)                                                                                                                              cTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGc   >  1:947705/1‑139 (MQ=255)                                                                                                                              cTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGc   <  2:947705/139‑1 (MQ=255)                                                                                                                              cTATCACTGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCa  <  1:1010124/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     TTTGCTGGAAGCGAGCGATGGAATCTTCCAGAGCGGCATCTTTGCCGGGGATAAATGTTTGCGTCATGAAAATCACTTTAGTCGTACGGAAAGCGCGCAATAATACGGGTTTTATCTCAAAGGCGCTATCACCGCCGCCATGCCGATGAACTGTTGACTATGCTTTTAGCGGATAAACCACGTTAAGAGCATAAAAATGAAGGCGTTCGATCTCCACCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCA  >  NC_000913/956566‑956830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑