Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,165,996	A→G	intergenic (+311/‑89)	ycfP → / → ndh	UPF0227 protein YcfP/NADH:quinone oxidoreductase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,165,996	0	A	G	100.0%	232.7 / NA	62	intergenic (+311/‑89)	ycfP/ndh	UPF0227 protein YcfP/NADH:quinone oxidoreductase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/43);  total (19/43)

GCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAACATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACGCCATTGAAAAAGATTGTGATTGTCGGCGGCGGTGCTGG  >  NC_000913/1165861‑1166131                                                                                                                                        |                                                                                                                                        gCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCAtt                                                                                                                                     >  2:184863/1‑140 (MQ=255)   tCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCAtttt                                                                                                                                   >  1:1329882/1‑140 (MQ=255)     aaaTAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCa                                                                                                                                 <  1:862000/140‑1 (MQ=255)     aaaTAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCa                                                                                                                                 <  1:960347/140‑1 (MQ=255)     aaaTAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCa                                                                                                                                 <  2:406791/140‑1 (MQ=255)     aaaTAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCa                                                                                                                                 <  2:780453/140‑1 (MQ=255)     aaaTAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCa                                                                                                                                 <  1:1098426/140‑1 (MQ=255)       ataataAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCAtt                                                                                                                               >  1:959002/1‑140 (MQ=255)        taataaACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTg                                                                                                                              <  2:94530/140‑1 (MQ=255)        taataaACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTg                                                                                                                              <  2:571058/140‑1 (MQ=255)          ataaacaataaacTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCt                                                                                                                            <  1:257713/140‑1 (MQ=255)               caataaacTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAAc                                                                                                                       <  2:1329882/140‑1 (MQ=255)               caataaacTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAAc                                                                                                                       <  1:553169/140‑1 (MQ=255)               caataaacTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAAc                                                                                                                       <  1:633011/140‑1 (MQ=255)                 ataaacTCTGTTTTTCGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCTTTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCt                                                                                                                     <  1:221664/140‑1 (MQ=255)                  taaacTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCtg                                                                                                                    >  1:917852/1‑140 (MQ=255)                   aaacTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCtgt                                                                                                                   >  2:702529/1‑140 (MQ=255)                   aaacTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCtgt                                                                                                                   <  1:647806/140‑1 (MQ=255)                   aaacTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCtgt                                                                                                                   <  2:1272340/140‑1 (MQ=255)                     acTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCtgttg                                                                                                                 >  2:1057258/1‑140 (MQ=255)                      ctctGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCtgttgt                                                                                                                <  2:917852/140‑1 (MQ=255)                      ctctGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCtgttgt                                                                                                                <  1:702529/140‑1 (MQ=255)                         tGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGttaa                                                                                                             >  2:730304/1‑140 (MQ=255)                           ttttttGATCTCGCCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGttaatt                                                                                                           <  1:852122/140‑1 (MQ=255)                           ttttttGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGttaatt                                                                                                           <  2:1302677/140‑1 (MQ=255)                           ttttttGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTGTCATTGCTGTAACCTGTTGttaatt                                                                                                           <  2:774156/140‑1 (MQ=255)                              tttGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAag                                                                                                        <  1:958636/140‑1 (MQ=255)                                tGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAagag                                                                                                      <  1:1057258/140‑1 (MQ=255)                                tGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAagag                                                                                                      <  1:794466/140‑1 (MQ=255)                                tGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAagag                                                                                                      >  1:629655/1‑140 (MQ=255)                                  aTCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCt                                                                                                    <  2:486604/140‑1 (MQ=255)                                  aTCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCt                                                                                                    <  2:493710/140‑1 (MQ=255)                                  aTCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCt                                                                                                    <  1:1093906/140‑1 (MQ=255)                                  aTCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCt                                                                                                    <  2:679952/140‑1 (MQ=255)                                  aTCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCt                                                                                                    <  1:184863/140‑1 (MQ=255)                                  aTCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCt                                                                                                    <  1:672495/140‑1 (MQ=255)                                   tctcACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAagag                                                                                                      >  1:333758/1‑137 (MQ=255)                                   tctcACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAagag                                                                                                      <  2:333758/137‑1 (MQ=255)                                   tctcACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTa                                                                                                   <  1:730304/140‑1 (MQ=255)                                   tctcACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTa                                                                                                   >  2:1246176/1‑140 (MQ=255)                                     tcACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATg                                                                                                 >  1:373994/1‑140 (MQ=255)                                               aaGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCa                                                                                       <  2:629655/140‑1 (MQ=255)                                                 gTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCt                                                                                                    <  1:775607/125‑1 (MQ=255)                                                 gTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCt                                                                                                    >  2:775607/1‑125 (MQ=255)                                                   cGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCattaa                                                                                   >  1:860638/1‑140 (MQ=255)                                                     ccTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCattaatt                                                                                 >  2:543819/1‑140 (MQ=255)                                                         tCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACa                                                                             <  1:682517/140‑1 (MQ=255)                                                          cTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACaa                                                                            >  1:693014/1‑140 (MQ=255)                                                              tCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTgg                                                                        <  1:1204160/140‑1 (MQ=255)                                                                  caacaaAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTtaa                                                                    <  1:88211/140‑1 (MQ=255)                                                                           ttGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTaa                                                           <  1:479248/140‑1 (MQ=255)                                                                               ttAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAgggg                                                       <  2:800408/140‑1 (MQ=255)                                                                                  aCATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTc                                                    <  2:373994/140‑1 (MQ=255)                                                                                  aCATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTc                                                    <  1:543819/140‑1 (MQ=255)                                                                                    aTCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCAc                                                  <  1:849160/140‑1 (MQ=255)                                                                                    aTCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCAc                                                  <  2:860638/140‑1 (MQ=255)                                                                                              gTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACg                                        >  1:1200840/1‑140 (MQ=255)                                                                                                               ttCATGTTATTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACGCCATTG‑AAAAgattgtg                      >  1:115250/1‑140 (MQ=255)                                                                                                                       aTTCTCAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACGCCATTGAAAAAGATTGTGATTGTcg               >  1:337229/1‑140 (MQ=255)                                                                                                                           tcAATAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACGCCATTGAAAAAGATTGTGATTGTcggcgg           <  2:959002/140‑1 (MQ=255)                                                                                                                              aTAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACGCCATTGAAAAAGATTGTGATTGTcggcggcgg        <  2:1172115/140‑1 (MQ=255)                                                                                                                               tAGCGAAGAGCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACGCCATTGAAAAAGATTGTGATTGTCGGCGGCGGt       >  1:537443/1‑140 (MQ=255)                                                                                                                                   gaagagCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACGCCATTG‑AAAAGATTGTGATTGTCGGCGGCGGTGCtgg  <  2:115250/140‑1 (MQ=255)                                                                                                                                    aagagCATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACGCCATTGAAAAAGATTGTGATTGTCGGCGGCGGTGCtgg  >  1:1244444/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTTGGTATGACCAATGCACCATTCATGTTATTCTCAATAGCGAAGAACATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTAATAAATTTAAGGGGGTCACGTTGACTACGCCATTGAAAAAGATTGTGATTGTCGGCGGCGGTGCTGG  >  NC_000913/1165861‑1166131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑