Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,253,492	A→G	S821P (TCC→CCC)	ycgV ←	putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,253,492	0	A	G	100.0%	326.4 / NA	89	S821P (TCC→CCC)	ycgV	putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (54/35);  total (54/35)

GCTGGCACGCCCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGACAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATCGC  >  NC_000913/1253355‑1253626                                                                                                                                          |                                                                                                                                       gCTGGCACGCCCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCa                                                                                                                                      <  1:1299948/140‑1 (MQ=255)  cTGGCACGCCCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTACGGCTCTATATAGAACCCATAACCAGTAGGGGGCAg                                                                                                                                     >  1:1416953/1‑140 (MQ=255)          ccccAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAAcc                                                                                                                             >  2:1419178/1‑140 (MQ=255)                 agcGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGccc                                                                                                                      <  1:1113647/140‑1 (MQ=255)                 agcGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGccc                                                                                                                      <  2:1072712/140‑1 (MQ=255)                     aTTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCt                                                                                                                  >  2:491606/1‑140 (MQ=255)                     aTTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCt                                                                                                                  >  1:480148/1‑140 (MQ=255)                     aTTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCt                                                                                                                  >  1:1441067/1‑140 (MQ=255)                     aTTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCt                                                                                                                  >  2:183983/1‑140 (MQ=255)                     aTTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCt                                                                                                                  >  1:633665/1‑140 (MQ=255)                     aTTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCt                                                                                                                  >  1:920918/1‑140 (MQ=255)                           aGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAgg                                                                                                            <  1:796842/140‑1 (MQ=255)                              gATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGgag                                                                                                         <  1:183983/140‑1 (MQ=255)                               aTTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGgaga                                                                                                        >  2:750640/1‑140 (MQ=255)                                  cAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGc                                                                                                     >  1:182328/1‑140 (MQ=255)                                           atTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattccat                                                                                            <  2:480148/140‑1 (MQ=255)                                               aGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATATCCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGc                                                                                        >  1:598077/1‑140 (MQ=255)                                               aGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGc                                                                                        >  1:488057/1‑140 (MQ=255)                                               aGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGc                                                                                        >  2:1150560/1‑140 (MQ=255)                                               aGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGc                                                                                        >  1:377199/1‑140 (MQ=255)                                               aGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGc                                                                                        >  2:1401193/1‑140 (MQ=255)                                                 gCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAg                                                                                      >  2:212010/1‑140 (MQ=255)                                                    aTTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattccat                                                                                            <  2:992664/131‑1 (MQ=255)                                                    aTTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattccat                                                                                            >  1:992664/1‑131 (MQ=255)                                                    aTTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGc                                                                                   >  2:73921/1‑140 (MQ=255)                                                    aTTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGc                                                                                   >  1:343087/1‑140 (MQ=255)                                                        cTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGtt                                                                               <  2:29921/140‑1 (MQ=255)                                                           gCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGc                                                                            >  2:1327434/1‑140 (MQ=255)                                                           gCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGc                                                                            >  2:918303/1‑140 (MQ=255)                                                                cATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTaa                                                                       <  2:182328/140‑1 (MQ=255)                                                                 aTAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAAc                                                                      >  1:1372172/1‑140 (MQ=255)                                                                    gTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                                                                   <  1:732446/140‑1 (MQ=255)                                                                    gTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                                                                   <  2:920918/140‑1 (MQ=255)                                                                    gTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                                                                   <  1:918303/140‑1 (MQ=255)                                                                       aTCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgtt                                                                >  1:835467/1‑140 (MQ=255)                                                                       aTCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCCCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgtt                                                                >  2:160452/1‑140 (MQ=255)                                                                         ctcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                                                                   >  2:491153/1‑135 (MQ=255)                                                                         ctcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                                                                   <  1:491153/135‑1 (MQ=255)                                                                         ctcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                                                                   <  1:334317/135‑1 (MQ=255)                                                                         ctcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                                                                   >  2:334317/1‑135 (MQ=255)                                                                          tcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgttgtt                                                             >  1:790100/1‑140 (MQ=255)                                                                          tcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCg                                                                  <  1:679851/135‑1 (MQ=255)                                                                          tcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCg                                                                  >  2:679851/1‑135 (MQ=255)                                                                              tCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGa                                                         <  1:1401193/140‑1 (MQ=255)                                                                              tCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGa                                                         <  1:212010/140‑1 (MQ=255)                                                                               cTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                                                                   >  1:1414620/1‑129 (MQ=255)                                                                               cTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                                                                   <  2:1414620/129‑1 (MQ=255)                                                                               cTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGAc                                                        >  2:351246/1‑140 (MQ=255)                                                                                  gtggCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgttgtt                                                             <  1:1301603/132‑1 (MQ=255)                                                                                  gtggCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgttgtt                                                             >  2:1301603/1‑132 (MQ=255)                                                                                  gtggCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGa                                                         <  2:1433657/136‑1 (MQ=255)                                                                                  gtggCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGa                                                         >  1:1433657/1‑136 (MQ=255)                                                                                   tggCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTc                                                    <  1:1150560/140‑1 (MQ=255)                                                                                    ggCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTcc                                                   >  2:1372442/1‑140 (MQ=255)                                                                                      cTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAg                                                 <  1:1327434/140‑1 (MQ=255)                                                                                       tgtatgtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGt                                                >  1:40433/1‑140 (MQ=255)                                                                                       tgtatgtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGt                                                >  2:230056/1‑140 (MQ=255)                                                                                           tgtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGt                                            <  2:1441067/140‑1 (MQ=255)                                                                                             taAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTgg                                          <  1:1399233/140‑1 (MQ=255)                                                                                              aaGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGa                                         <  2:1372172/140‑1 (MQ=255)                                                                                                   gggTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTa                                    >  2:443002/1‑140 (MQ=255)                                                                                                    ggTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTAt                                   >  2:632719/1‑140 (MQ=255)                                                                                                    ggTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTAt                                   >  2:46881/1‑140 (MQ=255)                                                                                                     gTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTAtt                                  >  2:499162/1‑140 (MQ=255)                                                                                                       ttGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTAtttt                                >  2:1296925/1‑140 (MQ=255)                                                                                                              tctATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGgcg                         <  1:647911/140‑1 (MQ=255)                                                                                                              tctATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGgcg                         <  1:443002/140‑1 (MQ=255)                                                                                                                 atataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGa                      <  1:1296925/140‑1 (MQ=255)                                                                                                                   ataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATg                    <  2:790100/140‑1 (MQ=255)                                                                                                                       aaCCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCttt                >  1:1368665/1‑140 (MQ=255)                                                                                                                       aaCCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCttt                >  2:409759/1‑140 (MQ=255)                                                                                                                       aaCCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCttt                >  1:766331/1‑140 (MQ=255)                                                                                                                       aaCCCATAACCAGTAGGGGGCAGGCTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCttt                >  2:147792/1‑140 (MQ=255)                                                                                                                         cccATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTta              >  1:538532/1‑140 (MQ=255)                                                                                                                         cccATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTta              >  1:463758/1‑140 (MQ=255)                                                                                                                          ccATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTtat             >  2:455181/1‑140 (MQ=255)                                                                                                                          ccATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTtat             <  2:343087/140‑1 (MQ=255)                                                                                                                            aTAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGAt                     >  2:368129/1‑130 (MQ=255)                                                                                                                            aTAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGAt                     <  1:368129/130‑1 (MQ=255)                                                                                                                            aTAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATaa           >  1:841482/1‑140 (MQ=255)                                                                                                                               aCCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACga        <  1:351246/140‑1 (MQ=255)                                                                                                                                   gTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATc    >  1:473682/1‑140 (MQ=255)                                                                                                                                   gTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATc    <  2:40433/140‑1 (MQ=255)                                                                                                                                   gTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATc    >  2:996808/1‑140 (MQ=255)                                                                                                                                    tAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATCg   >  1:43218/1‑140 (MQ=255)                                                                                                                                    tAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATCg   >  1:355469/1‑140 (MQ=255)                                                                                                                                    tAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATCg   >  1:54663/1‑140 (MQ=255)                                                                                                                                    tAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATCg   <  2:463758/140‑1 (MQ=255)                                                                                                                                     aGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATCGc  <  2:1147776/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GCTGGCACGCCCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGACAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTACGTGGAAACTATTTTTCTGGCGCGATGCTTTTATAACGAGATCGC  >  NC_000913/1253355‑1253626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑