Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,434,409	A→G	intergenic (‑3/+549)	ynaM ← / ← ttcC	Rac prophage; protein YnaM/putative tRNA 2‑thiocytidine (s(2)C32) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,434,409	0	A	G	100.0%	307.2 / NA	87	intergenic (‑3/+549)	ynaM/ttcC	Rac prophage; protein YnaM/putative tRNA 2‑thiocytidine (s(2)C32) synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/45);  total (42/45)

TCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGTTT  >  NC_000913/1434272‑1434544                                                                                                                                          |                                                                                                                                        tCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGc                                                                                                                                       >  2:339915/1‑140 (MQ=37) tCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTA‑‑GGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCt                                                                                                                                     <  1:1195254/140‑1 (MQ=255)  cTGATTCAGGATATGTTGACTTATATATCCGTTTTGTCTGGGTTATTGGTTATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGATCGAAGTGATTAACAGTATGCTGTTCATATGGcc                                                                                                                                      >  2:757019/1‑140 (MQ=11)  cTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGcc                                                                                                                                      >  2:758812/1‑140 (MQ=37)   tGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCt                                                                                                                                     <  2:1055066/140‑1 (MQ=37)   tGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCt                                                                                                                                     <  1:862820/140‑1 (MQ=255)    gATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTc                                                                                                                                    >  2:170955/1‑140 (MQ=37)    gATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTc                                                                                                                                    >  1:955703/1‑140 (MQ=37)     aTTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCg                                                                                                                                   >  1:325346/1‑140 (MQ=37)       tCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGaa                                                                                                                                 >  2:462231/1‑140 (MQ=37)           gATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAg                                                                                                                             >  2:990346/1‑140 (MQ=37)           gATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAg                                                                                                                             <  2:916180/140‑1 (MQ=255)           gATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAg                                                                                                                             >  1:68821/1‑140 (MQ=37)            atatGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGt                                                                                                                            <  2:245697/140‑1 (MQ=255)             tatGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTa                                                                                                                           <  1:339915/140‑1 (MQ=255)             tatGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTa                                                                                                                           <  2:1454409/140‑1 (MQ=255)             tatGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTAAGCTGTTCATATGGCCTCGAATTAGTa                                                                                                                           <  2:1424979/140‑1 (MQ=255)               tGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAAt                                                                                                                         >  2:919394/1‑140 (MQ=37)               tGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAAt                                                                                                                         <  1:665788/140‑1 (MQ=255)                gTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCt                                                                                                                                     >  1:151389/1‑127 (MQ=255)                gTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCt                                                                                                                                     <  2:151389/127‑1 (MQ=255)                gTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAAtg                                                                                                                        >  2:141703/1‑140 (MQ=37)                  tGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAAtgtg                                                                                                                      <  1:462231/140‑1 (MQ=38)                  tGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAAtgtg                                                                                                                      <  1:708974/140‑1 (MQ=37)                  tGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAAtgtg                                                                                                                      <  2:723194/140‑1 (MQ=255)                  tGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAAtgtg                                                                                                                      <  1:990346/140‑1 (MQ=255)                  tGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAAtgtg                                                                                                                      <  1:1180731/140‑1 (MQ=255)                    aCTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGtt                                                                                                                    <  1:391213/140‑1 (MQ=255)                    aCTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGtt                                                                                                                    <  2:166380/140‑1 (MQ=255)                    aCTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGtt                                                                                                                    <  2:525645/140‑1 (MQ=255)                    aCTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTAATATGGCCTCGAATTAGTAATGTGtt                                                                                                                    <  2:278576/140‑1 (MQ=255)                       tataCATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTtata                                                                                                                 <  1:919394/140‑1 (MQ=255)                       tataCATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTtata                                                                                                                 <  1:301110/140‑1 (MQ=255)                         taCATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTtatata                                                                                                               >  1:122954/1‑140 (MQ=38)                              cGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGata                                                                                                          >  1:194535/1‑140 (MQ=37)                              cGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGata                                                                                                          >  1:164400/1‑140 (MQ=38)                              cGGGTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGata                                                                                                          >  2:1254459/1‑140 (MQ=255)                                   ttGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGATTATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGtt                                                                                                     >  2:535417/1‑140 (MQ=255)                                   ttGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGtt                                                                                                     >  1:1049/1‑140 (MQ=38)                                    tGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTg                                                                                                    <  1:135553/140‑1 (MQ=37)                                               ttGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTAt                                                                                         >  2:887606/1‑140 (MQ=255)                                                  gATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCt                                                                                      <  2:955703/140‑1 (MQ=255)                                                    tatGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTgg                                                                                    >  2:1401512/1‑140 (MQ=255)                                                    tatGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTgg                                                                                    <  2:122954/140‑1 (MQ=255)                                                    tatGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTgg                                                                                    >  1:403015/1‑140 (MQ=255)                                                     atGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTggg                                                                                   <  1:887606/140‑1 (MQ=255)                                                     atGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTggg                                                                                   <  1:1434553/140‑1 (MQ=255)                                                     atGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTggg                                                                                   <  2:262400/140‑1 (MQ=255)                                                       gCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGgtg                                                                                 >  1:648410/1‑140 (MQ=255)                                                       gCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGgtg                                                                                 >  1:1333812/1‑140 (MQ=255)                                                             cccTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTa                                                                           <  1:1254459/140‑1 (MQ=255)                                                             cccTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTa                                                                           <  1:915214/140‑1 (MQ=255)                                                                  aTTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGtt                                                                      >  2:1421693/1‑140 (MQ=255)                                                                  aTTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGtt                                                                      <  2:1179603/140‑1 (MQ=255)                                                                    nnnnttaGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCg                                                                    <  2:1049/136‑1 (MQ=255)                                                                        ttaGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGa                                                                >  1:1382389/1‑140 (MQ=255)                                                                              cATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACa                                                          >  1:622569/1‑140 (MQ=255)                                                                                tGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACAtt                                                        <  1:248788/140‑1 (MQ=37)                                                                                 gACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTg                                                       <  2:1333812/140‑1 (MQ=38)                                                                                     aaaaaTGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGt                                                   <  2:817567/140‑1 (MQ=37)                                                                                     aaaaaTGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGt                                                   <  2:325346/140‑1 (MQ=38)                                                                                      aaaaTGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGtt                                                  <  2:194535/140‑1 (MQ=37)                                                                                       aaaTGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGttt                                                 >  2:1422021/1‑140 (MQ=38)                                                                                        aaTGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTc                                                <  1:779638/140‑1 (MQ=38)                                                                                         aTGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCa                                               >  2:899910/1‑140 (MQ=255)                                                                                          tGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAg                                              <  2:97271/140‑1 (MQ=38)                                                                                             tGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGta                                           <  1:1422021/140‑1 (MQ=37)                                                                                             tGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGta                                           <  2:462067/140‑1 (MQ=37)                                                                                               aaTATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGtata                                         <  1:259672/140‑1 (MQ=37)                                                                                                  atGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAg                                      <  1:758812/140‑1 (MQ=38)                                                                                                   tGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAgg                                     <  1:170955/140‑1 (MQ=37)                                                                                                     aTAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGaa                                   <  2:164400/140‑1 (MQ=38)                                                                                                           aGCGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTaa                             >  1:113702/1‑140 (MQ=37)                                                                                                             cGAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACa                           >  2:138228/1‑140 (MQ=25)                                                                                                              gAAGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAg                          >  2:175948/1‑140 (MQ=21)                                                                                                               aaGTGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGAGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAgg                         >  1:461833/1‑140 (MQ=18)                                                                                                                  tGATTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTgg                      <  2:1110928/140‑1 (MQ=17)                                                                                                                    aTTATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTggcg                    >  1:131032/1‑140 (MQ=18)                                                                                                                     ttATCAGTATGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTggcgg                   >  2:1311058/1‑140 (MQ=18)                                                                                                                              tGCTGTTCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCa          >  1:98138/1‑140 (MQ=21)                                                                                                                                   ttCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAg         <  1:361354/136‑1 (MQ=25)                                                                                                                                   ttCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAg         >  2:361354/1‑136 (MQ=25)                                                                                                                                   ttCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCgg     >  2:1450449/1‑140 (MQ=25)                                                                                                                                   ttCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCgg     >  2:847456/1‑140 (MQ=21)                                                                                                                                   ttCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCgg     >  1:732639/1‑140 (MQ=21)                                                                                                                                   ttCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCgg     >  1:716071/1‑140 (MQ=25)                                                                                                                                   ttCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCgg     >  1:1388686/1‑140 (MQ=25)                                                                                                                                   ttCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAA‑TTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGt    >  2:237924/1‑140 (MQ=255)                                                                                                                                    tCATATGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGt    >  2:1120344/1‑140 (MQ=25)                                                                                                                                      atatGGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGttt  >  2:581682/1‑140 (MQ=21)                                                                                                                                          |                                                                                                                                        TCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGTTT  >  NC_000913/1434272‑1434544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑