Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,489,101	T→C	R290R (CGT→CGC)	aldA →	aldehyde dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,489,101	0	T	C	100.0%	183.6 / NA	50	R290R (CGT→CGC)	aldA	aldehyde dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/23);  total (27/23)

CACCAAAGTGTGTCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGA  >  NC_000913/1488966‑1489235                                                                                                                                        |                                                                                                                                       cACCAAAGTGTGTCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGttt                                                                                                                                    <  2:190723/140‑1 (MQ=255) cACCAAAGTGTGTCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGttt                                                                                                                                    >  1:1451441/1‑140 (MQ=255)          gtgtCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGa                                                                                                                           <  2:544160/140‑1 (MQ=255)           tgtCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGaa                                                                                                                          <  1:338523/140‑1 (MQ=255)           tgtCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGaa                                                                                                                          <  1:859028/140‑1 (MQ=255)            gtCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGaaa                                                                                                                         >  2:982728/1‑140 (MQ=255)              cTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAgg                                                                                                                       <  1:103004/140‑1 (MQ=255)              cTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAgg                                                                                                                       <  1:1247271/140‑1 (MQ=255)              cTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAgg                                                                                                                       <  2:1055548/140‑1 (MQ=255)              cTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAgg                                                                                                                       <  1:982728/140‑1 (MQ=255)                      ggggggTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATg                                                                                                               <  1:1388652/140‑1 (MQ=255)                       gggggTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGa                                                                                                              >  2:514114/1‑140 (MQ=255)                            tAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGt                                                                                                         >  2:1457659/1‑140 (MQ=255)                                 cACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTc                                                                                                    <  1:1457659/140‑1 (MQ=255)                                    cAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAAt                                                                                                 <  2:998203/140‑1 (MQ=255)                                      gCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCg                                                                                               >  2:1361533/1‑140 (MQ=255)                                       cTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCgg                                                                                              <  1:1131752/140‑1 (MQ=255)                                                 ggACGATGCCGATCTTGAACTGGCAGACAAAGCAATCGTTGATTCACGCGTCCTTAATAGTGGGCAAGCGTCTAACTGAGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCAGCTGGGTGaaa                                                                                    >  2:875968/1‑139 (MQ=255)                                                  gACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGc                                                                                   >  2:1004538/1‑140 (MQ=255)                                                    cGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGa                                                                                 >  1:1162201/1‑140 (MQ=255)                                                       tGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGc                                                                              >  2:65558/1‑140 (MQ=255)                                                       tGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGc                                                                              >  2:747239/1‑140 (MQ=255)                                                        gCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCa                                                                             <  2:379469/140‑1 (MQ=255)                                                        gCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCa                                                                             <  1:1278374/140‑1 (MQ=255)                                                          cGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAgg                                                                           <  1:747239/140‑1 (MQ=255)                                                            aTCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCg                                                                         >  2:1402056/1‑140 (MQ=255)                                                                       gCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTgg                                                              >  2:876764/1‑140 (MQ=255)                                                                               aGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCCATTTTGTAAccccc                                                      >  1:411038/1‑139 (MQ=255)                                                                                  cATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTg                                                   <  1:441361/140‑1 (MQ=255)                                                                                     cGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTTAATTTGGTAAACCCCCTGaaa                                                >  2:441361/1‑139 (MQ=255)                                                                                       ttGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGc                                              <  1:876764/140‑1 (MQ=255)                                                                                                     aTTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGAt                                >  2:1309969/1‑140 (MQ=255)                                                                                                         aTAGTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATgggg                            <  1:110144/140‑1 (MQ=255)                                                                                                            gTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTTAATTTGGTAACCCCGCTGAACGCAACGACATTTCGATGGGGCCg                         >  2:1252767/1‑140 (MQ=255)                                                                                                            gTGGGCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCg                         <  1:1004538/140‑1 (MQ=255)                                                                                                              gggCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCCATTTTGTAAACCCCCTGAACGCAACCACAATGCGATGGGGCCCtt                       >  1:109587/1‑140 (MQ=255)                                                                                                              gggCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTTACCCCCCCGTAAGCCACGACATTGGGATGGGGCCGtt                       >  2:973864/1‑140 (MQ=255)                                                                                                              gggCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAACCCCGCTGGACGCCACGACATTGGCATGGGGCCGtt                       >  2:243973/1‑140 (MQ=255)                                                                                                               ggCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTAAGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTTAATTTTGTAACCCCCCTGAAAGCAACGACATTGCGGTGGGGGCGGTg                      >  2:957866/1‑140 (MQ=255)                                                                                                                gCAAGTGTGTAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCTATTTTGTAACCCCCCCGGACGCAACGCCATTTCGATGGGGCCGCTgg                     >  2:281908/1‑139 (MQ=255)                                                                                                                     tgtgtAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCTATTTTGGAAACCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAAc                >  1:525542/1‑140 (MQ=255)                                                                                                                         tAACTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAAcgccg            <  2:919895/140‑1 (MQ=255)                                                                                                                          aaCTGTGCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAAcgccgc           >  2:1134979/1‑140 (MQ=255)                                                                                                                              gtgCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCgcggcg       <  1:1361533/140‑1 (MQ=255)                                                                                                                               tgCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAACCCCGCTGAAAGCAAAGACAATTCCGTGGGGCCGTTGGTTATCGCCGCGgcgc      >  2:1195826/1‑140 (MQ=255)                                                                                                                               tgCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAACCCCCCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGgcgc      >  2:511334/1‑140 (MQ=255)                                                                                                                               tgCAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAAACCCCCCGAACGCCACGACATTTCGATGGGGCCGGTGATTAAAACCGCGgcgc      >  2:507879/1‑140 (MQ=255)                                                                                                                                 cAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTTAATTTTGTAACCCCCCTGAACGCCACGACATTGCGATGGGGGCGTTGATTAACGCCGCGGCGCTg    >  2:1440863/1‑140 (MQ=255)                                                                                                                                 cAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCgcggcg       <  2:757862/137‑1 (MQ=255)                                                                                                                                 cAGAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCgcggcg       >  1:757862/1‑137 (MQ=255)                                                                                                                                   gAACGCGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGa  <  1:1134979/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CACCAAAGTGTGTCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGATCTTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGA  >  NC_000913/1488966‑1489235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑