Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,858	A→G	Y245H (TAT→CAT)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,858	0	A	G	100.0%	264.5 / NA	71	Y245H (TAT→CAT)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/30);  total (41/30)

CACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTA  >  NC_000913/1541727‑1541993                                                                                                                                    |                                                                                                                                        cACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGAGATGATGTAGCTGGAGTTATGCCAGTCgg                                                                                                                                 <  2:280709/140‑1 (MQ=255)   cTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCa                                                                                                                               >  1:131642/1‑140 (MQ=255)     tCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGTCCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGa                                                                                                                             >  1:138634/1‑140 (MQ=255)     tCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGa                                                                                                                             >  1:522615/1‑140 (MQ=255)             gtCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTa                                                                                                                     >  2:628744/1‑140 (MQ=255)             gtCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTa                                                                                                                     >  2:264/1‑140 (MQ=255)              tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTAc                                                                                                                    >  1:1026719/1‑140 (MQ=255)              tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTAc                                                                                                                    >  1:973200/1‑140 (MQ=255)              tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTAc                                                                                                                    >  2:122122/1‑140 (MQ=255)              tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTAc                                                                                                                    >  2:1418694/1‑140 (MQ=255)              tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTAc                                                                                                                    >  1:1377639/1‑140 (MQ=255)              tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTAc                                                                                                                    >  1:1310270/1‑140 (MQ=255)              tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTAc                                                                                                                    >  2:545271/1‑140 (MQ=255)              tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTAc                                                                                                                    >  1:1125495/1‑140 (MQ=255)                  gggTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCg                                                                                                                >  2:1030266/1‑140 (MQ=255)                     tAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGtt                                                                                                             >  1:362240/1‑140 (MQ=255)                       aTGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTg                                                                                                           >  2:342132/1‑140 (MQ=255)                       aTGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTg                                                                                                           <  2:362240/140‑1 (MQ=255)                       aTGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTg                                                                                                           <  1:122122/140‑1 (MQ=255)                        tGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGc                                                                                                          >  2:298449/1‑140 (MQ=255)                        tGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGc                                                                                                          <  2:973200/140‑1 (MQ=255)                         ggCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCt                                                                                                         <  2:1377639/140‑1 (MQ=255)                         ggCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCt                                                                                                         <  2:1125495/140‑1 (MQ=255)                         ggCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCt                                                                                                         <  1:992066/140‑1 (MQ=255)                         ggCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCt                                                                                                         <  1:892400/140‑1 (MQ=255)                            gATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGc                                                                                                      >  2:982552/1‑140 (MQ=255)                             aTGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGcc                                                                                                     >  2:526709/1‑140 (MQ=255)                              tGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGccc                                                                                                    <  1:628744/140‑1 (MQ=255)                              tGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGccc                                                                                                    <  1:264/140‑1 (MQ=255)                               ggTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGcccc                                                                                                   <  1:1378823/140‑1 (MQ=255)                                   ttAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGt                                                                                               <  2:1310270/140‑1 (MQ=255)                                   ttAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGt                                                                                               <  2:1026719/140‑1 (MQ=255)                                          cTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGc                                                                                        >  2:893404/1‑140 (MQ=255)                                          cTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGc                                                                                        >  2:1420810/1‑140 (MQ=255)                                           tttGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCg                                                                                       >  1:575969/1‑140 (MQ=255)                                           tttGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCg                                                                                       >  1:156993/1‑140 (MQ=255)                                                  ggTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAggcggg                                                                                <  2:592167/139‑1 (MQ=255)                                                       ttCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCa                                                                           <  2:1285981/140‑1 (MQ=255)                                                       ttCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCa                                                                           <  1:1420810/140‑1 (MQ=255)                                                            tAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCg                                                                      >  2:1283189/1‑140 (MQ=255)                                                             aaaGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGc                                                                     <  1:1418694/140‑1 (MQ=255)                                                             aaaGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGc                                                                     <  1:545271/140‑1 (MQ=255)                                                                 aagTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATa                                                                 >  1:559600/1‑140 (MQ=255)                                                                  agTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCATGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATAc                                                                <  2:421049/140‑1 (MQ=255)                                                                   gTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATAcc                                                               >  2:959249/1‑140 (MQ=255)                                                                   gTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATAcc                                                               <  1:342132/140‑1 (MQ=255)                                                                      ggCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGt                                                            <  1:1283189/140‑1 (MQ=255)                                                                          tCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTa                                                        <  2:1159067/140‑1 (MQ=255)                                                                           ccGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAg                                                       >  1:596267/1‑140 (MQ=255)                                                                              gCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGaaa                                                    >  2:19075/1‑140 (MQ=255)                                                                                             gTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTgccg                                     <  1:526709/140‑1 (MQ=255)                                                                                                 gTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCaa                                 >  1:942052/1‑140 (MQ=255)                                                                                                  ttAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAag                                <  2:559600/140‑1 (MQ=255)                                                                                                  ttAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAag                                <  1:1194662/140‑1 (MQ=255)                                                                                                  ttAGACCACCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAag                                <  1:522575/140‑1 (MQ=255)                                                                                                   tAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAagc                               >  2:1389311/1‑140 (MQ=255)                                                                                                   tAGACCCCCAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAagc                               >  2:1343568/1‑140 (MQ=255)                                                                                                        ccccAGGCGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGa                          >  2:752502/1‑140 (MQ=255)                                                                                                               cGATGATGTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATaa                   >  1:108623/1‑140 (MQ=255)                                                                                                                 atgatgTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAAcg                 <  1:1030266/140‑1 (MQ=255)                                                                                                                  tgatgTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAAcgc                >  2:513544/1‑140 (MQ=255)                                                                                                                  tgatgTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAAcgc                >  1:1441616/1‑140 (MQ=255)                                                                                                                    atgTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGt              >  1:703439/1‑140 (MQ=255)                                                                                                                    atgTAGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGGCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCCAGCAACGACAAATAACCCGt              >  2:731117/1‑140 (MQ=255)                                                                                                                        aGCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcg          >  1:1149346/1‑140 (MQ=255)                                                                                                                         gCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcgg         <  2:596267/140‑1 (MQ=255)                                                                                                                         gCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcgg         <  2:942052/140‑1 (MQ=255)                                                                                                                         gCTGGAGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcgg         <  2:108623/140‑1 (MQ=255)                                                                                                                              aGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGgcggcg    >  1:1380506/1‑140 (MQ=255)                                                                                                                              aGTTATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCAGCCAAGCAGCGACAGATAACGCGTTCCGgcggcg    >  1:1259132/1‑140 (MQ=255)                                                                                                                                ttATGCCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTa  >  1:1275155/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        CACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTA  >  NC_000913/1541727‑1541993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑