Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,545,966	A→G	A21A (GCT→GCC)	yddL ←	putative uncharacterized protein YddL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,545,966	0	A	G	100.0%	182.0 / NA	50	A21A (GCT→GCC)	yddL	putative uncharacterized protein YddL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/24);  total (26/24)

ATTTGCGTTTCTCCTTTAAAGCCGAGACGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCAGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGAC  >  NC_000913/1545829‑1546101                                                                                                                                          |                                                                                                                                        aTTTGCGTTTCTCCTTTAAAGCCGAGACGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCggcggc                                                                                                                                       <  1:177402/140‑1 (MQ=255)  tttGCGTTTCTCCTTTAAAGCCGAGACGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGgcggcg                                                                                                                                      <  1:306453/140‑1 (MQ=255)   ttGCGTTTCTCCTTTAAAGCCGAGACGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCgt                                                                                                                                     <  1:848022/140‑1 (MQ=255)             ccTTTAAAGCCGAGACGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGtt                                                                                                                           <  2:488881/140‑1 (MQ=255)                ttAAAGCCGAGACGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGc                                                                                                                        <  1:1440065/140‑1 (MQ=255)                        gagaCGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTTTTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACa                                                                                                                >  1:1378211/1‑140 (MQ=255)                        gagaCGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACa                                                                                                                >  1:380611/1‑140 (MQ=255)                        gagaCGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACa                                                                                                                >  2:736754/1‑140 (MQ=255)                        gagaCGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACa                                                                                                                >  1:223079/1‑140 (MQ=255)                         agaCGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACaa                                                                                                               >  2:303000/1‑140 (MQ=255)                           aCGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAAc                                                                                                             <  2:1020254/140‑1 (MQ=255)                                     gTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAac                                                                                                   <  2:554288/140‑1 (MQ=255)                                     gTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGATAACAAACCAGTTACAac                                                                                                   <  2:1127209/140‑1 (MQ=255)                                        ttATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAaccac                                                                                                <  1:12129/140‑1 (MQ=255)                                        ttATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAaccac                                                                                                <  1:303000/140‑1 (MQ=255)                                        ttATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAaccac                                                                                                <  1:1133258/140‑1 (MQ=255)                                           tCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGc                                                                                             >  2:209344/1‑140 (MQ=255)                                           tCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGc                                                                                             <  2:223079/140‑1 (MQ=255)                                              ccATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAAc                                                                                          <  2:1254177/140‑1 (MQ=255)                                               cATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACt                                                                                         >  1:291558/1‑140 (MQ=255)                                                       cGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCATCTATCAATCCAGTTACAACCGCCGCAACTATTTTTaa                                                                                 <  2:318637/140‑1 (MQ=255)                                                         cTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAAtt                                                                               <  1:230908/140‑1 (MQ=255)                                                         cTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAAtt                                                                               >  2:1283939/1‑140 (MQ=255)                                                                    tAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAg                                                                    <  1:1027013/140‑1 (MQ=255)                                                                    tAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAg                                                                    >  1:384691/1‑140 (MQ=255)                                                                     aaaaTAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATCTCATTTAACAGa                                                                   <  2:576057/140‑1 (MQ=255)                                                                            cGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCttt                                                            >  2:1023166/1‑140 (MQ=255)                                                                             gTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCtttt                                                           >  2:1384909/1‑140 (MQ=255)                                                                                gagCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAAt                                                        <  1:736754/140‑1 (MQ=255)                                                                                 agCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAAtt                                                       >  1:692210/1‑140 (MQ=255)                                                                                 agCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAAtt                                                       >  1:261624/1‑140 (MQ=255)                                                                                   cGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTAt                                                     >  1:1383268/1‑140 (MQ=255)                                                                                        aCCTTGCCATAAAGGTCGAGTTTTTTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTa                                                <  2:1378211/140‑1 (MQ=255)                                                                                        aCCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTa                                                <  2:380611/140‑1 (MQ=255)                                                                                         ccTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTaa                                               <  2:1441607/140‑1 (MQ=255)                                                                                         ccTTGCCATAAAGGGCGAGATTATTACCATCCTTGTTATAGACTTTGGCGGCGTGTGCTACGGTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTaa                                               >  2:246146/1‑140 (MQ=255)                                                                                             gCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGt                                           >  1:20163/1‑140 (MQ=255)                                                                                                        cGAGTTTATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAAc                                >  1:1229934/1‑140 (MQ=255)                                                                                                            tttATTACCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAAt                            <  1:1023166/140‑1 (MQ=255)                                                                                                                ttaCCATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTa                        >  2:446964/1‑140 (MQ=255)                                                                                                                    cATCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACg                    >  1:653643/1‑140 (MQ=255)                                                                                                                      tCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGc                  >  1:1402026/1‑140 (MQ=255)                                                                                                                      tCCTTGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGc                  >  2:770145/1‑140 (MQ=255)                                                                                                                      tCCTTGTTATAGACTTCGGCGCCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGc                  >  1:962838/1‑140 (MQ=255)                                                                                                                       ccTTGTTATGGACGTCGGCAGCGTGTGCTACGTCCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCg                 <  1:1420170/140‑1 (MQ=255)                                                                                                                          tGTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCa              >  1:801019/1‑140 (MQ=255)                                                                                                                           gTTATAGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCAt             <  2:1383268/140‑1 (MQ=255)                                                                                                                              ataGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTta          <  2:384691/140‑1 (MQ=255)                                                                                                                              ataGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTta          >  1:1277444/1‑140 (MQ=255)                                                                                                                               taGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTtat         <  2:874027/140‑1 (MQ=255)                                                                                                                               taGACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTtat         <  2:962838/140‑1 (MQ=255)                                                                                                                                 gACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATaa       <  2:1402026/140‑1 (MQ=255)                                                                                                                                 gACTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATaa       <  2:682760/140‑1 (MQ=255)                                                                                                                                  aCTTCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATaaa      >  2:30128/1‑140 (MQ=255)                                                                                                                                    ttCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATg    >  2:1103921/1‑140 (MQ=255)                                                                                                                                     tCGGCGGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGa   >  1:197443/1‑140 (MQ=255)                                                                                                                                      cggcggCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCg                 <  2:1165003/125‑1 (MQ=255)                                                                                                                                      cggcggCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCg                 >  1:1165003/1‑125 (MQ=255)                                                                                                                                      cggcggCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGAc  >  1:845951/1‑140 (MQ=255)                                                                                                                                      cggcggCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGAc  >  2:1248514/1‑140 (MQ=255)                                                                                                                                      cggcggCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGAc  >  1:287566/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        ATTTGCGTTTCTCCTTTAAAGCCGAGACGGGCATAAGTTTTATCACCATCGTCACGCTTATCATCAGTAAAATAACGTAGAGCGGTAACCTTGCCATAAAGGTCGAGTTTATTACCATCCTTGTTATAGACTTCGGCAGCGTGTGCTACGTTCGCAGCTAACAAACCAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGAC  >  NC_000913/1545829‑1546101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑