Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,656,986	T→C	T158A (ACG→GCG)	ynfC ←	UPF0257 family lipoprotein YnfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,656,986	0	T	C	100.0%	276.3 / NA	75	T158A (ACG→GCG)	ynfC	UPF0257 family lipoprotein YnfC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/35);  total (40/35)

ACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCT  >  NC_000913/1656849‑1657117                                                                                                                                          |                                                                                                                                    aCTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCgg                                                                                                                                   >  2:813495/1‑140 (MQ=255)          ttCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCa                                                                                                                          <  1:813495/140‑1 (MQ=255)          ttCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCa                                                                                                                          <  1:1161923/140‑1 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     >  1:1047584/1‑140 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     <  2:977971/140‑1 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     >  2:686323/1‑140 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     >  2:486093/1‑140 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     >  2:1056927/1‑140 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     <  1:686323/140‑1 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     >  1:615540/1‑140 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     <  1:486093/140‑1 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     <  1:363507/140‑1 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     >  1:270734/1‑140 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     >  1:1037278/1‑140 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     >  1:1129722/1‑140 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     <  1:1131923/140‑1 (MQ=255)               aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGa                                                                                                                     <  1:1320167/140‑1 (MQ=255)                gCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTCCTTTTCGCGGTTTTACCCAGCGGAt                                                                                                                    <  1:67826/140‑1 (MQ=255)                gCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGAt                                                                                                                    <  1:927243/140‑1 (MQ=255)                   ctGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAAc                                                                                                                 >  2:1217088/1‑140 (MQ=255)                   ctGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAAc                                                                                                                 >  1:411560/1‑140 (MQ=255)                    tGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTAccc                                                                                                                           <  1:578709/129‑1 (MQ=255)                    tGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTAccc                                                                                                                           >  2:578709/1‑129 (MQ=255)                    tGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAAcc                                                                                                                <  2:1129722/140‑1 (MQ=255)                      ttttACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCt                                                                                                              <  1:108059/140‑1 (MQ=255)                      ttttACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCt                                                                                                              >  1:672944/1‑140 (MQ=255)                       tttACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTg                                                                                                             <  1:599257/140‑1 (MQ=255)                         tACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGat                                                                                                           <  2:315001/140‑1 (MQ=255)                                ccAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATa                                                                                                    >  1:642025/1‑140 (MQ=255)                                ccAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATa                                                                                                    >  1:800235/1‑140 (MQ=255)                                         tGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTc                                                                                           >  2:780822/1‑140 (MQ=255)                                            ttattCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCAt                                                                                        <  2:581343/140‑1 (MQ=255)                                            ttattCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCAt                                                                                        <  2:659833/140‑1 (MQ=255)                                             tattCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATc                                                                                       <  2:672944/140‑1 (MQ=255)                                               ttCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTg                                                                                     >  1:665161/1‑140 (MQ=255)                                                  agtaaagtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGGAt                                                                                  >  2:1316279/1‑140 (MQ=255)                                                  agtaaagtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCAt                                                                                  >  1:672611/1‑140 (MQ=255)                                                  agtaaagtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCAt                                                                                  >  2:1289725/1‑140 (MQ=255)                                                     aaagtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCAtttg                                                                               >  2:1394951/1‑140 (MQ=255)                                                     aaagtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCAtttg                                                                               >  2:564627/1‑140 (MQ=255)                                                       agtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCAtttgtt                                                                             <  2:949618/140‑1 (MQ=255)                                                          aaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTtgc                                                                          >  1:483919/1‑140 (MQ=255)                                                            ccGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTtgctg                                                                        <  2:1047584/140‑1 (MQ=255)                                                            ccGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTtgctg                                                                        <  2:597544/140‑1 (MQ=255)                                                            ccGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTtgctg                                                                        >  1:58443/1‑140 (MQ=255)                                                            ccGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGATTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTtgctg                                                                        >  1:954429/1‑140 (MQ=255)                                                                  tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCt                                                                  <  2:1382717/140‑1 (MQ=255)                                                                   gtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCtt                                                                 >  1:1222413/1‑140 (MQ=255)                                                                            ttttttGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGa                                                        >  1:889980/1‑140 (MQ=255)                                                                            ttttttGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGa                                                        >  2:654096/1‑140 (MQ=255)                                                                             tttttGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGaa                                                       <  2:665161/140‑1 (MQ=255)                                                                             tttttGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGaa                                                       <  1:780822/140‑1 (MQ=255)                                                                                ttGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGcc                                                    <  2:672611/140‑1 (MQ=255)                                                                                    tCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTa                                                <  2:800235/140‑1 (MQ=255)                                                                                        aTCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTatcat                                            >  2:808486/1‑140 (MQ=255)                                                                                           cGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCgg                                         <  1:1394951/140‑1 (MQ=255)                                                                                                aTGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTcc                                    <  2:1222413/140‑1 (MQ=255)                                                                                                  ggcgtggcgCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCa                                  >  2:642655/1‑140 (MQ=255)                                                                                                    cgtggcgCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAAc                                >  2:424296/1‑140 (MQ=255)                                                                                                      tggcgCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTc                              >  1:1059178/1‑140 (MQ=255)                                                                                                       ggcgCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTc                                     <  1:1255951/132‑1 (MQ=255)                                                                                                       ggcgCTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTc                                     >  2:1255951/1‑132 (MQ=255)                                                                                                        gcgcTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCAc                            >  2:1182945/1‑140 (MQ=255)                                                                                                         cgcTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCAcc                           <  2:1059178/140‑1 (MQ=255)                                                                                                          gcTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCAccc                          <  1:564627/140‑1 (MQ=255)                                                                                                          gcTGACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACACAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCAccc                          <  2:954429/140‑1 (MQ=255)                                                                                                             gACAGATAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCgg                       <  1:1056927/140‑1 (MQ=255)                                                                                                                  aTAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGaa                  <  1:444726/140‑1 (MQ=255)                                                                                                                  aTAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGaa                  >  1:487516/1‑140 (MQ=255)                                                                                                                  aTAATGTTTTGTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGaa                  >  2:444726/1‑140 (MQ=255)                                                                                                                            gTCGTTACTTTTCGCGGTTTTACCCAGCGGATACCCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTg        >  2:1239191/1‑140 (MQ=255)                                                                                                                            gTCGTTACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTg        >  2:1259740/1‑140 (MQ=255)                                                                                                                                ttACTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAg    >  1:819365/1‑140 (MQ=255)                                                                                                                                  aCTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCt  >  1:1431691/1‑140 (MQ=255)                                                                                                                                  aCTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCt  <  1:808486/140‑1 (MQ=255)                                                                                                                                  aCTTTTCGCGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCt  >  2:906567/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    ACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCT  >  NC_000913/1656849‑1657117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑