Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,016,912	T→C	V120A (GTT→GCT)	fliI →	flagellum‑specific ATP synthase FliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,016,912	0	T	C	100.0%	96.2 / NA	28	V120A (GTT→GCT)	fliI	flagellum‑specific ATP synthase FliI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/13);  total (15/13)

CTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCC  >  NC_000913/2016782‑2017047                                                                                                                                   |                                                                                                                                        cTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACgg                                                                                                                                <  2:395085/140‑1 (MQ=255) cTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACgg                                                                                                                                <  2:1276587/140‑1 (MQ=255)            gCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAAc                                                                                                                     <  2:822976/140‑1 (MQ=255)              cGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCg                                                                                                                   >  2:862628/1‑140 (MQ=255)                                   tCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTccccc                                                                                              >  2:208075/1‑140 (MQ=255)                                    ccTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCg                                                                                             >  1:537234/1‑140 (MQ=255)                                       gCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATa                                                                                          <  2:84436/140‑1 (MQ=255)                                        cccGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATac                                                                                         >  1:1158412/1‑140 (MQ=255)                                        cccGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATac                                                                                         >  2:267071/1‑140 (MQ=255)                                                      ttATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTg                                                                           <  2:792136/140‑1 (MQ=255)                                                            cAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGa                                                                     >  1:893079/1‑140 (MQ=255)                                                              aaaaCATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGAtt                                                                   >  1:601547/1‑140 (MQ=255)                                                              aaaaCATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGAtt                                                                   >  1:187583/1‑140 (MQ=255)                                                                   aTTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTAcccc                                                              <  1:267071/140‑1 (MQ=255)                                                                   aTTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTAcccc                                                              <  2:524192/140‑1 (MQ=255)                                                                    tttCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCg                                                             <  1:208075/140‑1 (MQ=255)                                                                                   tGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTg                                              <  2:187583/140‑1 (MQ=255)                                                                                      aaaGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCaa                                           <  2:893079/140‑1 (MQ=255)                                                                                       aaGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAAc                                          >  1:1359568/1‑140 (MQ=255)                                                                                             cAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGCAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTacac                                    >  2:1391201/1‑140 (MQ=255)                                                                                              aaGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACAcc                                   <  2:1359568/140‑1 (MQ=255)                                                                                               aGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCg                                  >  1:1288747/1‑140 (MQ=255)                                                                                                      gCCGCTCGGTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAAc                           >  1:1104964/1‑140 (MQ=255)                                                                                                             ggTCCGGCGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCt                    <  1:759839/140‑1 (MQ=255)                                                                                                                   gCGTAATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTTCCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGacac              <  2:1288747/140‑1 (MQ=255)                                                                                                                    cGTTATTAGGTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGTTGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGTTTGAACATGTGCTGGACAcc             >  2:1336420/1‑140 (MQ=255)                                                                                                                             gTCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTgcgc    >  2:1345905/1‑140 (MQ=255)                                                                                                                              tCGCGCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGcc   >  2:627651/1‑140 (MQ=255)                                                                                                                               cgcgCTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGccc  <  2:270055/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        CTGTTTTTAATGCCGCTGGAGGAAGTCGAAGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCC  >  NC_000913/2016782‑2017047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑