Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,222,185	A→G	M1M (ATG→GTG) †	dld →	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,222,185	0	A	G	100.0%	270.9 / NA	72	M1M (ATG→GTG) †	dld	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/28);  total (44/28)

TTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGAC  >  NC_000913/2222049‑2222319                                                                                                                                         |                                                                                                                                       ttGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAgtgt                                                                                                                                     <  2:266922/140‑1 (MQ=255) ctGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAgtgt                                                                                                                                     >  1:805946/2‑140 (MQ=255)   gCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCt                                                                                                                                   >  1:384355/1‑140 (MQ=255)   gCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCt                                                                                                                                   <  2:384355/140‑1 (MQ=255)   gCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCt                                                                                                                                   <  2:950366/140‑1 (MQ=255)   gCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCt                                                                                                                                   >  1:950366/1‑140 (MQ=255)      aTAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTcc                                                                                                                                <  2:1131613/140‑1 (MQ=255)      aTAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCAAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTcc                                                                                                                                <  1:1168998/140‑1 (MQ=255)          gCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGa                                                                                                                            <  2:585452/140‑1 (MQ=255)                   agagagaAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGacaaca                                                                                                                       >  2:15983/1‑136 (MQ=255)                   agagagaAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGacaaca                                                                                                                       <  1:15983/136‑1 (MQ=255)                   agagagaAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTg                                                                                                                   >  1:655402/1‑140 (MQ=255)                   agagagaAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTg                                                                                                                   >  2:660468/1‑140 (MQ=255)                               aaTGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCt                                                                                                       >  2:733985/1‑140 (MQ=255)                                   cTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCttttt                                                                                                   >  2:518356/1‑140 (MQ=255)                                      aGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTtgaa                                                                                                <  1:660468/140‑1 (MQ=255)                                       gTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTtgaat                                                                                               >  1:1363030/1‑140 (MQ=255)                                         ttATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTtgaatga                                                                                             <  2:335829/140‑1 (MQ=255)                                                cTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCt                                                                                      >  2:1400722/1‑140 (MQ=255)                                                cTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCt                                                                                      <  1:733985/140‑1 (MQ=255)                                                 tGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTc                                                                                     >  2:996082/1‑140 (MQ=255)                                                  gAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCg                                                                                    >  1:1107311/1‑140 (MQ=255)                                                  gAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCg                                                                                    >  2:518378/1‑140 (MQ=255)                                                  gAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCg                                                                                    >  1:316319/1‑140 (MQ=255)                                                  gAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCg                                                                                    >  2:1081136/1‑140 (MQ=255)                                                   aaTTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGt                                                                                   <  2:1353962/140‑1 (MQ=255)                                                           aaaTATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTggg                                                                           <  1:996082/140‑1 (MQ=255)                                                                     tCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCacac                                                                 <  1:10890/140‑1 (MQ=255)                                                                     tCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCacac                                                                 <  1:518378/140‑1 (MQ=255)                                                                           aaTTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTc                                                           >  1:1452324/1‑140 (MQ=255)                                                                            aTTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCa                                                          >  1:1267855/1‑140 (MQ=255)                                                                                        ggCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCaa                                              >  2:35716/1‑140 (MQ=255)                                                                                        ggCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCaa                                              >  2:23420/1‑140 (MQ=255)                                                                                        ggCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCaa                                              >  2:484834/1‑140 (MQ=255)                                                                                          cgcTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCaaaa                                            >  1:1037025/1‑140 (MQ=255)                                                                                          cgcTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCaaaa                                            >  2:969000/1‑140 (MQ=255)                                                                                          cgcTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCaaaa                                            >  2:55699/1‑140 (MQ=255)                                                                                          cgcTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCaaaa                                            >  1:731945/1‑140 (MQ=255)                                                                                          cgcTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCaaaa                                            >  1:483791/1‑140 (MQ=255)                                                                                             tATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACg                                         >  2:455838/1‑140 (MQ=255)                                                                                                tCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGcc                                      <  1:55699/140‑1 (MQ=255)                                                                                                tCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGcc                                      <  2:1107311/140‑1 (MQ=255)                                                                                                  tAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCg                                    <  1:1231748/140‑1 (MQ=255)                                                                                                   aGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGc                                   <  2:316319/140‑1 (MQ=255)                                                                                                   aGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGAAAAAACGGCCCGc                                   <  1:1081136/140‑1 (MQ=255)                                                                                                    gTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCt                                  >  2:305799/1‑140 (MQ=255)                                                                                                     tttGTGATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTa                                 >  1:442202/1‑140 (MQ=255)                                                                                                       tgtgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATc                               <  1:1432898/140‑1 (MQ=255)                                                                                                       tgtgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATc                               >  1:1224191/1‑140 (MQ=255)                                                                                                       tgtgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATc                               <  1:223435/140‑1 (MQ=255)                                                                                                       tgtgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATc                               <  1:305799/140‑1 (MQ=255)                                                                                                         tgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGc                             >  1:369581/1‑140 (MQ=255)                                                                                                         tgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGc                             >  2:946749/1‑140 (MQ=255)                                                                                                         tgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGc                             >  2:752938/1‑140 (MQ=255)                                                                                                         tgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGc                             >  1:250592/1‑140 (MQ=255)                                                                                                         tgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGc                             >  1:27295/1‑140 (MQ=255)                                                                                                         tgATATTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGc                             >  2:1258608/1‑140 (MQ=255)                                                                                                            tatTTTTTCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAg                          <  2:483791/140‑1 (MQ=255)                                                                                                                 tttCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCtt                     <  1:35716/140‑1 (MQ=255)                                                                                                                 tttCGCCACCACAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCtt                     <  1:969000/140‑1 (MQ=255)                                                                                                                      ccaccaCAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGtt                <  1:752938/140‑1 (MQ=255)                                                                                                                      ccaccaCAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGtt                <  2:27295/140‑1 (MQ=255)                                                                                                                      ccaccaCAAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGtt                <  2:1037025/140‑1 (MQ=255)                                                                                                                           acaAGGAGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGt           <  2:1267855/140‑1 (MQ=255)                                                                                                                                 aGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGc     >  1:953133/1‑140 (MQ=255)                                                                                                                                 aGTGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGc     >  2:1425273/1‑140 (MQ=255)                                                                                                                                   tGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGgaga   >  1:30099/1‑140 (MQ=255)                                                                                                                                   tGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGa   >  2:360829/1‑140 (MQ=255)                                                                                                                                   tGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGa   >  2:456208/1‑140 (MQ=255)                                                                                                                                   tGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGa   >  1:971380/1‑140 (MQ=255)                                                                                                                                   tGGAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGa   >  2:1003774/1‑140 (MQ=255)                                                                                                                                    ggAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGAc  >  1:1083066/1‑140 (MQ=255)                                                                                                                                    ggAAAGTGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGAc  >  1:358009/1‑140 (MQ=255)                                                                                                                                    ggAAAGTGTCATCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGAc  >  1:174349/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGAC  >  NC_000913/2222049‑2222319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑