Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,240,766	T→C	E301E (GAA→GAG)	galS ←	DNA‑binding transcriptional dual regulator GalS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,240,766	0	T	C	100.0%	364.9 / NA	97	E301E (GAA→GAG)	galS	DNA‑binding transcriptional dual regulator GalS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/50);  total (47/50)

TACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCAATGCCATTATCTTTT  >  NC_000913/2240629‑2240901                                                                                                                                          |                                                                                                                                        tACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTc                                                                                                                                       <  1:163930/140‑1 (MQ=255)        ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCt                                                                                                                                >  2:425024/1‑140 (MQ=255)         cTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTa                                                                                                                               >  1:459213/1‑140 (MQ=255)             tttGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAAttt                                                                                                                           <  1:346060/140‑1 (MQ=255)               tgttgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCg                                                                                                                         <  2:1079429/140‑1 (MQ=255)                   gAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCAt                                                                                                                     >  2:64782/1‑140 (MQ=255)                    aGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCAtt                                                                                                                    <  1:665345/140‑1 (MQ=255)                      ttAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGa                                                                                                                  <  1:425024/140‑1 (MQ=255)                      ttAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGa                                                                                                                  <  1:64782/140‑1 (MQ=255)                        aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAg                                                                                                                <  2:352219/140‑1 (MQ=255)                           gATCTCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                                             <  2:834735/140‑1 (MQ=255)                           gATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                                             <  1:1021266/140‑1 (MQ=255)                            aTCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAAt                                                                                                            >  2:578100/1‑140 (MQ=255)                            aTCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAAt                                                                                                            <  2:307767/140‑1 (MQ=255)                             tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATg                                                                                                           <  2:663616/140‑1 (MQ=255)                              cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATgg                                                                                                          >  2:279102/1‑140 (MQ=255)                              cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATgg                                                                                                          >  2:35704/1‑140 (MQ=255)                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCa                                                                                                              >  1:646867/1‑134 (MQ=255)                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCa                                                                                                              <  2:646867/134‑1 (MQ=255)                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                                        >  1:263339/1‑140 (MQ=255)                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                                        >  2:328818/1‑140 (MQ=255)                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                                        <  1:948697/140‑1 (MQ=255)                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                                        >  1:839931/1‑140 (MQ=255)                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                                        >  1:1278503/1‑140 (MQ=255)                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                                        >  1:635234/1‑140 (MQ=255)                                  gccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATa                                                                                                      <  1:382829/140‑1 (MQ=255)                                    cgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAAc                                                                                                    >  1:1440535/1‑140 (MQ=255)                                       aTTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                                                 <  2:9802/140‑1 (MQ=255)                                       aTTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                                                 <  2:1440535/140‑1 (MQ=255)                                       aTTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                                                 <  1:952596/140‑1 (MQ=255)                                       aTTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                                                 <  1:816240/140‑1 (MQ=255)                                       aTTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                                                 <  2:635234/140‑1 (MQ=255)                                       aTTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                                                 <  1:438303/140‑1 (MQ=255)                                         tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg                                                                                               <  1:399102/140‑1 (MQ=255)                                           tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGt                                                                                             >  2:139552/1‑140 (MQ=255)                                           tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGt                                                                                             >  2:298016/1‑140 (MQ=255)                                                tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTa                                                                                        >  2:491389/1‑140 (MQ=255)                                                  ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAAt                                                                                      >  2:1307775/1‑140 (MQ=255)                                                       aCAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCgg                                                                                 >  1:52190/1‑140 (MQ=255)                                                        cAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCggg                                                                                <  2:52190/140‑1 (MQ=255)                                                         aGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                                               <  1:279102/140‑1 (MQ=255)                                                           aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCg                                                                             >  1:1074629/1‑140 (MQ=255)                                                           aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCg                                                                             <  2:459213/140‑1 (MQ=255)                                                             tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGgt                                                                           >  1:411823/1‑140 (MQ=255)                                                                cGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTa                                                                        >  1:404981/1‑140 (MQ=255)                                                                 gACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTaa                                                                       <  2:404981/140‑1 (MQ=255)                                                                     cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACggg                                                                   <  2:639883/140‑1 (MQ=255)                                                                     cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc                                                                  <  1:993760/140‑1 (MQ=255)                                                                      aCTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc                                                                  <  1:298016/140‑1 (MQ=255)                                                                        tAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGa                                                                <  1:491389/140‑1 (MQ=255)                                                                        tAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGa                                                                <  1:139552/140‑1 (MQ=255)                                                                         aaCGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                                               <  1:328818/140‑1 (MQ=255)                                                                          aCGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCg                                                                 <  2:691640/137‑1 (MQ=255)                                                                          aCGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCg                                                                 >  1:691640/1‑137 (MQ=255)                                                                                gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAata                                                        <  2:1278503/140‑1 (MQ=255)                                                                                gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCAATGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAata                                                        >  2:1376334/1‑140 (MQ=255)                                                                                    aaaaCAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATatcat                                                    >  2:516130/1‑140 (MQ=255)                                                                                    aaaaCAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATatcat                                                    >  1:470535/1‑140 (MQ=255)                                                                                      aaCAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCg                                                  >  1:596261/1‑140 (MQ=255)                                                                                        cAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaa                                                <  2:470535/140‑1 (MQ=255)                                                                                        cAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaa                                                <  1:718115/140‑1 (MQ=255)                                                                                          gTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAAc                                              <  1:516130/140‑1 (MQ=255)                                                                                           tggctggcACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAAcc                                             >  1:1187696/1‑140 (MQ=255)                                                                                           tggctggcACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAAcc                                             >  2:1333878/1‑140 (MQ=255)                                                                                            ggctggcACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg                                            <  1:951109/140‑1 (MQ=255)                                                                                             gctggcACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                                           <  1:1026053/140‑1 (MQ=255)                                                                                             gctggcACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                                           <  1:35704/140‑1 (MQ=255)                                                                                             gctggcACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                                           <  1:10216/140‑1 (MQ=255)                                                                                             gctggcACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                                           <  2:454617/140‑1 (MQ=255)                                                                                                ggcACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgatga                                        <  1:1333878/140‑1 (MQ=255)                                                                                                 gcACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgatgat                                       >  1:824558/1‑140 (MQ=255)                                                                                                     gAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag                                   <  1:1008744/140‑1 (MQ=255)                                                                                                     gAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag                                   <  1:1376334/140‑1 (MQ=255)                                                                                                       ggATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgagag                                 >  2:661328/1‑140 (MQ=255)                                                                                                          tCAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAAACGATGATTGAGAGATg                              >  2:938412/1‑140 (MQ=255)                                                                                                           cAATATTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGt                             >  1:565922/1‑140 (MQ=255)                                                                                                             atatTGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa                           <  2:411823/140‑1 (MQ=255)                                                                                                                 tGCCTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa                       >  2:137980/1‑140 (MQ=255)                                                                                                                   ccTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCGTTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAt                     >  2:354072/1‑140 (MQ=255)                                                                                                                   ccTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa                           >  2:40898/1‑134 (MQ=255)                                                                                                                   ccTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa                           <  1:40898/134‑1 (MQ=255)                                                                                                                   ccTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAt                     >  2:1000885/1‑140 (MQ=255)                                                                                                                   ccTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAt                     >  2:448803/1‑140 (MQ=255)                                                                                                                   ccTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAt                     >  1:190568/1‑140 (MQ=255)                                                                                                                   ccTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAt                     >  2:523838/1‑140 (MQ=255)                                                                                                                   ccTGCTGCCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAt                     >  2:646776/1‑140 (MQ=255)                                                                                                                     tgctgcCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCg                   >  1:1068544/1‑140 (MQ=255)                                                                                                                      gctgcCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGc                  >  2:613584/1‑140 (MQ=255)                                                                                                                       ctgcCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTAGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCa                 <  1:1307775/140‑1 (MQ=255)                                                                                                                        tgcCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCaa                >  2:401794/1‑140 (MQ=255)                                                                                                                        tgcCCCCTGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCaa                >  2:447739/1‑140 (MQ=255)                                                                                                                               tGCAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCAATGCCAtt         <  1:646776/140‑1 (MQ=255)                                                                                                                                 cAAGGCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCAATGCCATTAt       >  2:460589/1‑140 (MQ=255)                                                                                                                                    ggCCAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCAATGCCATTATCtt    >  1:130848/1‑140 (MQ=255)                                                                                                                                    ggCCAGCTCGGTGGCTAATTTCCCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCAATGCCATTa        >  2:1437117/1‑136 (MQ=255)                                                                                                                                    ggCCAGCTCGGTGGCTAATTTCCCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCAATGCCATTa        <  1:1437117/136‑1 (MQ=255)                                                                                                                                      ccAGCTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCAATGCCATTATCtttt  <  2:190568/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAATCGCAATGCCATTATCTTTT  >  NC_000913/2240629‑2240901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑