Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,297,466	A→G	V60A (GTT→GCT)	ccmA ←	heme trafficking system ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,297,466	0	A	G	100.0%	293.5 / NA	80	V60A (GTT→GCT)	ccmA	heme trafficking system ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/40);  total (40/40)

CCATCGCGATGATAAAAGTGCAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAACCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAATAAGGTTCGTTC  >  NC_000913/2297334‑2297602                                                                                                                                     |                                                                                                                                         ccATCGCGATGATAAAAGTGCAGATTTTCTAACGCCGTCAGCTGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGcc                                                                                                                                   >  1:832955/1‑140 (MQ=255) ccATCGCGATGATAAAAGTGCAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGcc                                                                                                                                   <  2:718492/140‑1 (MQ=255)      gcgATGATAAAAGTGCAGATTTTCTAACGCCGTCAGCTGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGt                                                                                                                              <  2:832955/140‑1 (MQ=255)      gcgATGATAAAAGTGCAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGt                                                                                                                              >  1:474971/1‑140 (MQ=255)           gatAAAAGTGCAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAggg                                                                                                                         <  1:932884/140‑1 (MQ=255)            atAAAAGTGCAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGc                                                                                                                        >  1:1136341/1‑140 (MQ=255)               aaaGTGCAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCgag                                                                                                                     <  2:473749/140‑1 (MQ=255)                aaGTGCAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCgaga                                                                                                                    >  2:740727/1‑140 (MQ=255)                  gTGCAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACa                                                                                                                  <  2:359324/140‑1 (MQ=255)                     cAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAAcc                                                                                                               >  1:1137534/1‑140 (MQ=255)                      aGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAAccc                                                                                                              >  1:602597/1‑140 (MQ=255)                      aGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAAccc                                                                                                              <  1:158766/140‑1 (MQ=255)                               aaCGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCaa                                                                                                     <  1:49902/140‑1 (MQ=255)                                  gCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACg                                                                                                  <  2:82045/140‑1 (MQ=255)                                  gCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACg                                                                                                  >  1:470937/1‑140 (MQ=255)                                     gTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGgag                                                                                               <  2:324960/140‑1 (MQ=255)                                      tCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGgaga                                                                                              >  2:976162/1‑140 (MQ=255)                                      tCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGgaga                                                                                              >  2:141683/1‑140 (MQ=255)                                            gggTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACACCCCCGTCAGCAAACGGAGAAGCGtt                                                                                        <  1:1118412/140‑1 (MQ=255)                                              gTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGt                                                                                      >  2:591219/1‑140 (MQ=255)                                                        ccGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCcgcg                                                                            >  2:931230/1‑140 (MQ=255)                                                         cGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCgcgc                                                                           >  2:367832/1‑140 (MQ=255)                                                         cGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCgcgc                                                                           >  2:1324281/1‑140 (MQ=255)                                                          ggCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGcc                                                                          >  2:525038/1‑140 (MQ=255)                                                               aTGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGc                                                                     <  1:976162/140‑1 (MQ=255)                                                                tGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCt                                                                    >  1:947511/1‑140 (MQ=255)                                                                    cTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCg                                                                <  2:602597/140‑1 (MQ=255)                                                                      aTCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGt                                                              <  2:972580/140‑1 (MQ=255)                                                                      aTCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGt                                                              >  2:343477/1‑140 (MQ=255)                                                                      aTCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGt                                                              <  1:1324281/140‑1 (MQ=255)                                                                      aTCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGt                                                              <  1:233975/140‑1 (MQ=255)                                                                      aTCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGt                                                              <  2:680499/140‑1 (MQ=255)                                                                      aTCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGt                                                              <  1:931230/140‑1 (MQ=255)                                                                      aTCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCACGCGCCGTTGCTACCGGt                                                              <  1:740727/140‑1 (MQ=255)                                                                       tCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCgg                                                               >  2:34982/1‑138 (MQ=255)                                                                       tCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCgg                                                               <  1:34982/138‑1 (MQ=255)                                                                         cATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGAt                                                           <  1:367832/140‑1 (MQ=255)                                                                          aTAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGAtt                                                          <  1:525038/140‑1 (MQ=255)                                                                          aTAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGAtt                                                          <  1:440863/140‑1 (MQ=255)                                                                          aTAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGAtt                                                          <  1:141683/140‑1 (MQ=255)                                                                            aaCAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTg                                                        >  2:1055093/1‑140 (MQ=255)                                                                              cAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTa                                                      >  2:1187121/1‑140 (MQ=255)                                                                                ggTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTAcc                                                    <  2:470937/140‑1 (MQ=255)                                                                                 gTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGc                                                                     >  1:1257974/1‑122 (MQ=255)                                                                                 gTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGc                                                                     <  2:1257974/122‑1 (MQ=255)                                                                                 gTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTAccc                                                   >  2:11081/1‑140 (MQ=255)                                                                                 gTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTAccc                                                   >  1:1278437/1‑140 (MQ=255)                                                                                 gTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTAccc                                                   >  2:90084/1‑140 (MQ=255)                                                                                 gTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTAccc                                                   >  2:369095/1‑140 (MQ=255)                                                                                 gTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTAccc                                                   >  2:1091645/1‑140 (MQ=255)                                                                                 gTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTAccc                                                   >  1:787431/1‑140 (MQ=255)                                                                                   tttGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCAc                                                 >  1:596998/1‑140 (MQ=255)                                                                                    ttGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCAct                                                <  1:1055093/140‑1 (MQ=255)                                                                                         ggTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCGCTCTCCt                                           <  1:1091645/140‑1 (MQ=255)                                                                                           tAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGc                                         >  1:937435/1‑140 (MQ=255)                                                                                            aGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCg                                        <  2:937435/140‑1 (MQ=255)                                                                                            aGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCg                                        <  2:1278437/140‑1 (MQ=255)                                                                                              cTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGtt                                      <  1:343477/140‑1 (MQ=255)                                                                                                gTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCa                                    <  1:11081/140‑1 (MQ=255)                                                                                                gTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCa                                    <  2:1333564/140‑1 (MQ=255)                                                                                                gTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCa                                    <  2:787431/140‑1 (MQ=255)                                                                                                   gcgcACCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCg                                 <  2:1398054/140‑1 (MQ=255)                                                                                                       aCCTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTaaa                             <  1:591219/140‑1 (MQ=255)                                                                                                         cTGATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATg                           >  2:337329/1‑140 (MQ=255)                                                                                                           gATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGtt                                      >  1:850940/1‑127 (MQ=255)                                                                                                           gATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGtt                                      <  2:850940/127‑1 (MQ=255)                                                                                                           gATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGAc                         >  2:309922/1‑140 (MQ=255)                                                                                                           gATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGAc                         <  2:947511/140‑1 (MQ=255)                                                                                                           gATGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGAc                         >  2:164681/1‑140 (MQ=255)                                                                                                            aTGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACa                        <  2:596998/140‑1 (MQ=255)                                                                                                            aTGCAAGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACa                        <  1:90084/140‑1 (MQ=255)                                                                                                                aaGGGCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACACCCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTCCCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGcc                    <  2:859355/140‑1 (MQ=255)                                                                                                                  gggCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCAc                  >  1:988210/1‑140 (MQ=255)                                                                                                                   agCTGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACt                 >  2:743941/2‑140 (MQ=255)                                                                                                                      tGCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTaaa              >  1:914620/1‑140 (MQ=255)                                                                                                                       gCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAGGCCACTAAAt             >  1:58196/1‑140 (MQ=255)                                                                                                                       gCCCTTGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAAt             >  2:428044/1‑140 (MQ=255)                                                                                                                           ttGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAATAAgg         <  1:369095/140‑1 (MQ=255)                                                                                                                           ttGCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAATAAgg         <  1:164681/140‑1 (MQ=255)                                                                                                                             gCCAGATAGCCTCGCCTGAATCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGGGCCGTTGCTACCGGGGATTTGGACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAATAAGgtt       >  2:1096675/1‑140 (MQ=255)                                                                                                                             gCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAATAAgggt       >  1:1180812/1‑138 (MQ=255)                                                                                                                             gCCAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAATAAGgtt       >  2:902253/1‑140 (MQ=255)                                                                                                                               cAGAGAGCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAATAAGgttcg     <  1:968105/140‑1 (MQ=255)                                                                                                                                  agagCCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAATAAGgttcgttc  <  2:914620/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CCATCGCGATGATAAAAGTGCAGATTTTCTAACGCCGTCAGCCGGGTTTTGATCCCCGGCTGATGGCCTATCCATAACAGGTTTTGATGGTAGCTGTCGCGTACCTGATGCAAGGGCTGCCCTTGCCAGAGAACCTCGCCTGCGTCAGGGCGAGACAACCCCGTCAGCAAACGGAGAAGCGTTGTCTTCCCCGCGCCGTTGCTACCGGTGATTTGTACCCACTCTCCTGCGTTCAGCGTAAATGACAAGCCACTAAATAAGGTTCGTTC  >  NC_000913/2297334‑2297602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑