Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,660,486	G→A	A349V (GCA→GTA)	iscS ←	cysteine desulfurase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,660,486	0	G	A	100.0%	295.7 / NA	85	A349V (GCA→GTA)	iscS	cysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/39);  total (46/39)

CACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTG  >  NC_000913/2660350‑2660617                                                                                                                                         |                                                                                                                                    cACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCa                                                                                                                                  <  2:621308/140‑1 (MQ=255)   cGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGc                                                                                                                                >  2:1195417/1‑140 (MQ=255)   cGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGc                                                                                                                                >  2:805751/1‑140 (MQ=255)          cTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCtcg                                                                                                                             >  2:557040/1‑136 (MQ=255)          cTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCtcg                                                                                                                             <  1:557040/136‑1 (MQ=255)          cTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTcgtcgt                                                                                                                         >  1:150018/1‑140 (MQ=255)          cTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTcgtcgt                                                                                                                         >  1:1321830/1‑140 (MQ=255)           ttGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGtt                                                                                                                        <  2:116282/140‑1 (MQ=255)           ttGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGtt                                                                                                                        <  2:1297193/140‑1 (MQ=255)           ttGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGtt                                                                                                                        <  2:174069/140‑1 (MQ=255)               aCATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGc                                                                                                                    >  1:324327/1‑140 (MQ=255)                    tCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAg                                                                                                               >  2:997100/1‑140 (MQ=255)                     cccACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAgc                                                                                                              >  2:80792/1‑140 (MQ=255)                        acaGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAgcgcg                                                                                                           <  2:531205/140‑1 (MQ=255)                          aGCGGAGAAAGGTCACGCAGACGACCGATGAATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAgcgcgcg                                                                                                         >  2:188893/1‑140 (MQ=255)                            cGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgca                                                                                                       <  1:80792/140‑1 (MQ=255)                            cGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgca                                                                                                       <  1:805751/140‑1 (MQ=255)                            cGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgca                                                                                                       <  1:174687/140‑1 (MQ=255)                            cGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgca                                                                                                       <  1:1324202/140‑1 (MQ=255)                            cGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgca                                                                                                       <  2:688872/140‑1 (MQ=255)                              gagaAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgcagc                                                                                                     <  1:551316/140‑1 (MQ=255)                               agaAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgcagca                                                                                                    >  1:456826/1‑140 (MQ=255)                               agaAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgcagca                                                                                                    >  1:1104680/1‑140 (MQ=255)                               agaAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgcagca                                                                                                    >  2:71196/1‑140 (MQ=255)                                 aaaGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACg                                                                                                  >  2:1015924/1‑140 (MQ=255)                                      tCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGa                                                                                             <  1:1195417/140‑1 (MQ=255)                                      tCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGa                                                                                             >  1:255091/1‑140 (MQ=255)                                      tCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGa                                                                                             >  2:76123/1‑140 (MQ=255)                                      tCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGa                                                                                             <  2:456826/140‑1 (MQ=255)                                       cACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGAc                                                                                            <  1:386911/140‑1 (MQ=255)                                          gCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGt                                                                                         >  1:502153/1‑140 (MQ=255)                                             gacgacCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgca                                                                                                       >  2:868007/1‑123 (MQ=255)                                             gacgacCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCgca                                                                                                       <  1:868007/123‑1 (MQ=255)                                                gacCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAg                                                                                    <  2:1295764/139‑1 (MQ=255)                                                gacCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAg                                                                                    >  1:1295764/1‑139 (MQ=255)                                                  ccGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCt                                                                                 <  1:71196/140‑1 (MQ=255)                                                  ccGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCt                                                                                 <  2:1104680/140‑1 (MQ=255)                                                  ccGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCt                                                                                 <  1:594716/140‑1 (MQ=255)                                                    gATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTg                                                                               >  2:1007572/1‑140 (MQ=255)                                                        gATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCt                                                                                 >  2:1213199/1‑134 (MQ=255)                                                        gATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCt                                                                                 <  1:1213199/134‑1 (MQ=255)                                                        gATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGa                                                                           >  1:763735/1‑140 (MQ=255)                                                        gATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGa                                                                           >  1:1062342/1‑140 (MQ=255)                                                            tACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTa                                                                       <  1:997100/140‑1 (MQ=255)                                                            tACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTa                                                                       <  2:710956/140‑1 (MQ=255)                                                            tACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGAAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTa                                                                       >  2:737616/1‑140 (MQ=255)                                                                   taactCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCgg                                                                <  2:502153/140‑1 (MQ=255)                                                                     actCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGaa                                                              <  1:76123/140‑1 (MQ=255)                                                                     actCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGaa                                                              >  1:238014/1‑140 (MQ=255)                                                                             gtgtAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTgaaga                                                      <  1:1007572/140‑1 (MQ=255)                                                                              tgtAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTgaagaa                                                     >  2:573594/1‑140 (MQ=255)                                                                              tgtAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTgaagaa                                                     >  1:601790/1‑140 (MQ=255)                                                                               gtAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGaaa                                                    >  1:1452633/1‑140 (MQ=255)                                                                                  gTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTg                                                 <  2:1442854/140‑1 (MQ=255)                                                                                  gTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTg                                                 >  1:742380/1‑140 (MQ=255)                                                                                     gATCTCTTCTTCAGTAGTAAAATGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGa                                              >  2:815254/1‑140 (MQ=255)                                                                                       tctcTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCATGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAgg                                            >  2:755365/1‑140 (MQ=255)                                                                                       tctcTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAgg                                            >  2:887930/1‑140 (MQ=255)                                                                                            tcttcAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCttt                                       <  2:1062342/140‑1 (MQ=255)                                                                                            tcttcAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCttt                                       >  1:1118541/1‑140 (MQ=255)                                                                                             cttcAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTc                                      >  2:22469/1‑140 (MQ=255)                                                                                              ttcAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCa                                     <  2:255091/140‑1 (MQ=255)                                                                                                   tagtaAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGcc                                >  2:1365727/1‑140 (MQ=255)                                                                                                   tagtaAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGcc                                >  1:1194067/1‑140 (MQ=255)                                                                                                   tagtaAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCCGAAGAAACTGCGAGGTCTTTCAGCGcc                                >  2:523642/1‑140 (MQ=255)                                                                                                     gtaAAACGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCAt                              >  1:1318089/1‑140 (MQ=255)                                                                                                        aaaCGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAAt                           <  1:573594/140‑1 (MQ=255)                                                                                                        aaaCGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAAt                           >  2:241364/1‑140 (MQ=255)                                                                                                         aaCGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATc                          <  1:1103496/140‑1 (MQ=255)                                                                                                         aaCGACCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATc                          <  2:1032732/140‑1 (MQ=255)                                                                                                             aCCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCg                      >  1:726645/1‑140 (MQ=255)                                                                                                             aCCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCg                      >  2:1288838/1‑140 (MQ=255)                                                                                                             aCCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCg                      >  1:164687/1‑140 (MQ=255)                                                                                                             aCCTAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCg                      >  2:1004529/1‑140 (MQ=255)                                                                                                                tAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACt                   >  2:425039/1‑140 (MQ=255)                                                                                                                tAAAGAGAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACt                   <  1:241364/140‑1 (MQ=255)                                                                                                                   agagAAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCAc                <  2:1154731/140‑1 (MQ=255)                                                                                                                    gagaAACGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCAcc               <  1:1004529/140‑1 (MQ=255)                                                                                                                       aaaCGGACAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTc            >  2:1405233/1‑140 (MQ=255)                                                                                                                          cGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAAc         <  1:737616/140‑1 (MQ=255)                                                                                                                          cGGATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAAc         <  1:22469/140‑1 (MQ=255)                                                                                                                            gATAGAGCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGt       >  1:49333/1‑140 (MQ=255)                                                                                                                                gagCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGtt   <  2:238014/140‑1 (MQ=255)                                                                                                                                gagCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACATGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGtt   <  2:613668/140‑1 (MQ=255)                                                                                                                                 agCTATGTACCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTg  >  1:630529/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    CACGCCCTGCTTGTACATTTCCCACAGCGGAGAAAGGTCACGCAGACGACCGATGGATTTACGAACTAACTCGATGGTGTAGTCGATCTCTTCTTCAGTAGTAAAACGACCTAAAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTG  >  NC_000913/2660350‑2660617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑