Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,857,298	C→A	A69E (GCG→GAG)	mutS →	DNA mismatch repair protein MutS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,857,298	0	C	A	100.0%	243.0 / NA	69	A69E (GCG→GAG)	mutS	DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/40);  total (29/40)

ATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGT  >  NC_000913/2857163‑2857433                                                                                                                                        |                                                                                                                                        aTCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAgggg                                                                                                                                     <  1:326925/140‑1 (MQ=255)   cccGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGAt                                                                                                                                   <  2:357578/140‑1 (MQ=255)   cccGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGAt                                                                                                                                   <  2:1363398/140‑1 (MQ=255)    ccGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAgg                                                                                                                                       >  2:1197910/1‑135 (MQ=255)     cGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTc                                                                                                                                 >  2:1352524/1‑140 (MQ=255)          tCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTAc                                                                                                                            >  1:1314916/1‑140 (MQ=255)          tCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTAc                                                                                                                            >  1:156818/1‑140 (MQ=255)             tgctgTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGa                                                                                                                                    <  1:1150201/129‑1 (MQ=255)             tgctgTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGa                                                                                                                                    >  2:1150201/1‑129 (MQ=255)             tgctgTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCAt                                                                                                                         >  1:1196054/1‑140 (MQ=255)             tgctgTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCAt                                                                                                                         >  2:414445/1‑140 (MQ=255)              gctgTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATg                                                                                                                        <  1:1002746/140‑1 (MQ=255)                  ttttACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGt                                                                                                                    >  1:1313792/1‑140 (MQ=255)                       ccGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGaaa                                                                                                               >  1:1438511/1‑140 (MQ=255)                         ggATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAAc                                                                                                             >  1:1454614/1‑140 (MQ=255)                           aTGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTa                                                                                                           <  1:275711/140‑1 (MQ=255)                            tGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTAt                                                                                                          <  2:188551/140‑1 (MQ=255)                            tGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTAt                                                                                                          <  2:219790/140‑1 (MQ=255)                                tGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCg                                                                                                      <  2:968488/140‑1 (MQ=255)                                tGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCCTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCg                                                                                                      >  2:987016/1‑140 (MQ=255)                                tGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGGCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACGATGCGGTGGAAAACTATCTCg                                                                                                      <  2:195624/140‑1 (MQ=255)                                   tttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCa                                                                                                   <  2:790262/140‑1 (MQ=255)                                   tttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCa                                                                                                   <  2:1454614/140‑1 (MQ=255)                                    ttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCaa                                                                                                  <  2:1438511/140‑1 (MQ=255)                                        aTGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTg                                                                                              <  1:54233/140‑1 (MQ=255)                                         tGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTgg                                                                                             >  1:670026/1‑140 (MQ=255)                                          gAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGt                                                                                            <  1:1049692/140‑1 (MQ=255)                                          gAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGt                                                                                            <  1:1344014/140‑1 (MQ=255)                                          gAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGt                                                                                            <  1:1417382/140‑1 (MQ=255)                                          gAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGt                                                                                            <  1:765184/140‑1 (MQ=255)                                            aCTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGa                                                                                          <  2:1313792/140‑1 (MQ=255)                                             cTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGaa                                                                                         <  1:406983/140‑1 (MQ=255)                                               gTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATc                                                                                       <  2:1071831/140‑1 (MQ=255)                                               gTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATc                                                                                       <  1:414445/140‑1 (MQ=255)                                                ttttATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCa                                                                                      >  2:289488/1‑140 (MQ=255)                                                ttttATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCa                                                                                      >  1:831754/1‑140 (MQ=255)                                                 tttATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAg                                                                                     <  2:670026/140‑1 (MQ=255)                                                    aTGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGga                                                                                  <  2:383798/140‑1 (MQ=255)                                                      gacgacGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGgaga                                                                                <  1:1402870/140‑1 (MQ=255)                                                          acgCAAAACGCGCGTCGCAACTGCTGGATTTTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCAGCTCCCGTAGGGGGGGATTTCCTACCATTCGGTGGGAAACTATCCCGCCAAACTGGCGATACAGGtagtgaac                                                                            >  2:1402870/1‑135 (MQ=255)                                                          acgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGt                                                                                            <  1:50083/124‑1 (MQ=255)                                                          acgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGt                                                                                            >  2:50083/1‑124 (MQ=255)                                                          acgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTcc                                                                            <  2:1314916/140‑1 (MQ=255)                                                          acgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTcc                                                                            >  1:60367/1‑140 (MQ=255)                                                          acgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTcc                                                                            <  2:156818/140‑1 (MQ=255)                                                          acgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTcc                                                                            >  1:154927/1‑140 (MQ=255)                                                          acgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTcc                                                                            >  2:432600/1‑140 (MQ=255)                                                           cgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCg                                                                           <  1:289488/140‑1 (MQ=255)                                                                     gcgTCGCAACTGTTGGATATTTCACTGTCCAAACGGGGTGCTTCGGCGGTAGAGCTGATCCCGATGGAGGGGATTCCATACCATGCGGTTGAGAACTATCTCGCCAAACTGTTGAATCAGAGAGAGTCCGTTGCCATCTg                                                                 <  2:1161753/140‑1 (MQ=255)                                                                                   ggATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCg                                                   >  2:690579/1‑140 (MQ=255)                                                                                           cACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCg                                           >  2:796551/1‑140 (MQ=255)                                                                                             cTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGAc                                         <  1:851981/140‑1 (MQ=255)                                                                                              tGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGAcc                                        >  2:313921/1‑140 (MQ=255)                                                                                                 ccAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGc                                     <  1:1352524/140‑1 (MQ=255)                                                                                                   aaaCGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCaa                                   <  2:831754/140‑1 (MQ=255)                                                                                                   aaaCGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCaa                                   <  2:406447/140‑1 (MQ=255)                                                                                                     aCGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAg                                 >  1:324616/1‑140 (MQ=255)                                                                                                             cTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGtt                         <  1:796551/140‑1 (MQ=255)                                                                                                             cTTCGGCGGGAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGtt                         <  2:1288646/140‑1 (MQ=255)                                                                                                                    gggAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCa                  <  2:154927/140‑1 (MQ=255)                                                                                                                    gggAGAGCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCa                  <  1:287892/140‑1 (MQ=255)                                                                                                                     ggAGAGCCGATCCCGATTGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCaa                 >  1:451080/1‑140 (MQ=255)                                                                                                                         agCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGtt             >  1:673401/1‑140 (MQ=255)                                                                                                                         agCCGATCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGtt             >  1:162751/1‑140 (MQ=255)                                                                                                                         agCCGATCCCGATGGAGGGGATTCCCTACCATGCGATGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGtt             >  2:583526/1‑140 (MQ=255)                                                                                                                              aTCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCg        <  1:690579/140‑1 (MQ=255)                                                                                                                               tCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGt       <  2:662897/140‑1 (MQ=255)                                                                                                                               tCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGt       >  2:690868/1‑140 (MQ=255)                                                                                                                               tCCCGATGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGt       <  1:268582/140‑1 (MQ=255)                                                                                                                                    aTGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGt  >  2:276479/1‑140 (MQ=255)                                                                                                                                    aTGGAGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGt  >  2:863090/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGT  >  NC_000913/2857163‑2857433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑