Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,899,808	A→G	S156P (TCT→CCT)	ygcW ←	putative deoxygluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,899,808	0	A	G	100.0%	292.6 / NA	78	S156P (TCT→CCT)	ygcW	putative deoxygluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/28);  total (50/28)

TGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGACCGAAGTCCA  >  NC_000913/2899674‑2899942                                                                                                                                       |                                                                                                                                       tGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACa                                                                                                                                   <  2:1449480/140‑1 (MQ=255)  gCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACaa                                                                                                                                  >  2:900184/1‑140 (MQ=255)    ataataGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATg                                                                                                                                >  1:955369/1‑140 (MQ=255)     taataGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGa                                                                                                                               >  2:59755/1‑140 (MQ=255)       ataGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAAc                                                                                                                             <  1:570360/140‑1 (MQ=255)         aGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAg                                                                                                                           <  1:59755/140‑1 (MQ=255)           ccAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAGTAAGCTTTGGTGAACTCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGat                                                                                                                         >  1:925223/1‑140 (MQ=255)           ccAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGat                                                                                                                         >  1:425643/1‑140 (MQ=255)             aGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGatat                                                                                                                       >  2:1343715/1‑140 (MQ=255)             aGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGatat                                                                                                                       >  1:605979/1‑140 (MQ=255)                ggCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGaa                                                                                                                              <  2:791133/130‑1 (MQ=255)                ggCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGaa                                                                                                                              >  1:791133/1‑130 (MQ=255)                ggCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATtaa                                                                                                                    >  1:29417/1‑140 (MQ=255)                    aTACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTaataat                                                                                                                >  2:1183741/1‑140 (MQ=255)                    aTACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTaataat                                                                                                                >  1:326546/1‑140 (MQ=255)                        cATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTg                                                                                                            <  2:117651/140‑1 (MQ=255)                         aTTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGc                                                                                                           <  2:533153/140‑1 (MQ=255)                           ttACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCg                                                                                                         <  2:277910/140‑1 (MQ=255)                               cTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCttt                                                                                                     >  1:1306432/1‑140 (MQ=255)                                    tattatattGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGc                                                                                                <  2:1016278/140‑1 (MQ=255)                                     attatattGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCg                                                                                               <  2:912729/140‑1 (MQ=255)                                       tatattGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCggg                                                                                             >  1:44085/1‑140 (MQ=255)                                         tattGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGAt                                                                                           >  2:845901/1‑140 (MQ=255)                                            tGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCAt                                                                                        >  2:535732/1‑140 (MQ=255)                                            tGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCAt                                                                                        >  2:569868/1‑140 (MQ=255)                                              aCCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATaa                                                                                      >  1:227904/1‑140 (MQ=255)                                              aCCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATaa                                                                                      >  1:611491/1‑140 (MQ=255)                                              aCCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATaa                                                                                      >  2:808111/1‑140 (MQ=255)                                              aCCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATaa                                                                                      >  2:1290147/1‑140 (MQ=255)                                                 tAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAAttt                                                                                   >  1:1412160/1‑140 (MQ=255)                                                   gTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAAttttt                                                                                 >  2:1186455/1‑140 (MQ=255)                                                   gTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAAttttt                                                                                 >  2:1379101/1‑140 (MQ=255)                                                       atcaCAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAg                                                                             <  2:1452659/140‑1 (MQ=255)                                                       atcaCAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAg                                                                             <  2:1363651/140‑1 (MQ=255)                                                       atcaCAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAg                                                                             <  2:717708/140‑1 (MQ=255)                                                        tcaCAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGc                                                                            >  1:4531/1‑140 (MQ=255)                                                        tcaCAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGc                                                                            >  1:318164/1‑140 (MQ=255)                                                         cacaATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAAtttt                                                                                  <  1:1068534/133‑1 (MQ=255)                                                         cacaATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAAtttt                                                                                  >  2:1068534/1‑133 (MQ=255)                                                              tAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCa                                                                              >  1:1041849/1‑132 (MQ=255)                                                              tAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCa                                                                              <  2:1041849/132‑1 (MQ=255)                                                              tAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATa                                                                      >  2:1225010/1‑140 (MQ=255)                                                                   tttGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTa                                                                 >  2:626914/1‑140 (MQ=255)                                                                      ggTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACt                                                              >  2:1028525/1‑140 (MQ=255)                                                                       gTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTc                                                             >  1:498817/1‑140 (MQ=255)                                                                          aaCCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGaa                                                          >  1:1260939/1‑140 (MQ=255)                                                                            cccGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATg                                                        >  2:931599/1‑140 (MQ=255)                                                                            cccGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATg                                                        >  1:1042493/1‑140 (MQ=255)                                                                               ggCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATgcgg                                                     >  1:1435273/1‑140 (MQ=255)                                                                                   agagCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGt                                                 <  1:422487/140‑1 (MQ=255)                                                                                       cATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAgg                                             <  2:326546/140‑1 (MQ=255)                                                                                         tGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGtt                                           <  2:925223/140‑1 (MQ=255)                                                                                          gTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTc                                                >  2:1144220/1‑134 (MQ=255)                                                                                          gTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTc                                                <  1:1144220/134‑1 (MQ=255)                                                                                             tAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTcaca                                       <  1:626914/140‑1 (MQ=255)                                                                                               gTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACatc                                     >  1:1135270/1‑140 (MQ=255)                                                                                                 ggCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACatcaa                                   >  2:358689/1‑140 (MQ=255)                                                                                                  gCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACatcaat                                  >  1:106567/1‑140 (MQ=255)                                                                                                     gAATATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAatcat                               <  2:106567/140‑1 (MQ=255)                                                                                                      aaTATGCAGGTGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAatcatc                              >  2:1358199/1‑140 (MQ=255)                                                                                                                tGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTTAGGTTGACATCGATCATCGGATCCCAgc                    >  1:1230111/1‑139 (MQ=255)                                                                                                                tGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGt                    >  1:472117/1‑140 (MQ=255)                                                                                                                tGACCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGt                    >  2:651189/1‑140 (MQ=255)                                                                                                                  aCCATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCg                  >  1:858784/1‑140 (MQ=255)                                                                                                                   ccATTGTCCACCTAAGTAAGGGAACAATTAACAGATATTAATAATTTTCCCGCTGTTCTGCGGGATCATAAATTTTGCAGTTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCgg                 <  2:738292/140‑1 (MQ=255)                                                                                                                   ccATTGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCgg                 >  2:1368170/1‑140 (MQ=255)                                                                                                                       tGTCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACg             >  1:44206/1‑140 (MQ=255)                                                                                                                         tCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCa                      <  1:844597/129‑1 (MQ=255)                                                                                                                         tCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCa                      >  2:844597/1‑129 (MQ=255)                                                                                                                         tCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCAc              <  1:1025623/137‑1 (MQ=255)                                                                                                                         tCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCAc              >  2:1025623/1‑137 (MQ=255)                                                                                                                         tCCACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGAc           >  2:388592/1‑140 (MQ=255)                                                                                                                           cACCTAAGTAAGGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGACCg         <  2:858784/140‑1 (MQ=255)                                                                                                                                aagtaagGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGACCGAAGTc    <  2:44085/140‑1 (MQ=255)                                                                                                                                 agtaagGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGACCGAAGTcc   >  2:4793/1‑140 (MQ=255)                                                                                                                                  gtaagGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGACCGAAGTCCa  <  2:1135270/140‑1 (MQ=255)                                                                                                                                  gtaagGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGACCGAAGTCCa  <  2:472117/140‑1 (MQ=255)                                                                                                                                  gtaagGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGACCGAAGTCCa  <  1:592493/140‑1 (MQ=255)                                                                                                                                  gtaagGGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCAGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGACCGAAGTCCa  <  2:425643/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTTCATCACAATAAGCTTTGGTGAACCCGGCAAGAGCATGTTTAGTGGCAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGGTTCACATCAATCATCGGATCCCAGTCGGCACGACCGAAGTCCA  >  NC_000913/2899674‑2899942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑