Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	418,900	C→T	H338Y (CAT→TAT)	phoR →	sensory histidine kinase PhoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	418,900	0	C	T	100.0%	270.6 / NA	77	H338Y (CAT→TAT)	phoR	sensory histidine kinase PhoR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/37);  total (40/37)

TGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTC  >  NC_000913/418769‑419030                                                                                                                                    |                                                                                                                                   tGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCAcc                                                                                                                            >  2:1293066/1‑140 (MQ=255)  gAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCg                                                                                                                           <  2:603098/140‑1 (MQ=255)   aGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGt                                                                                                                          >  2:576137/1‑140 (MQ=255)   aGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGt                                                                                                                          >  2:1456871/1‑140 (MQ=255)       aGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGc                                                                                                                      >  1:396803/1‑140 (MQ=255)         aaaaaaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTg                                                                                                                    >  1:163506/1‑140 (MQ=255)             aaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAg                                                                                                                >  1:615222/1‑140 (MQ=255)             aaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAg                                                                                                                >  1:981604/1‑140 (MQ=255)                aGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGGACGTATATCACCGTGCGCTGGCAGCGa                                                                                                             <  2:1054985/140‑1 (MQ=255)                        aCCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCa                                                                                                     <  2:232278/140‑1 (MQ=255)                        aCCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCa                                                                                                     <  1:677765/140‑1 (MQ=255)                        aCCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCa                                                                                                     <  1:869488/140‑1 (MQ=255)                        aCCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCa                                                                                                     <  1:971504/140‑1 (MQ=255)                        aCCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCa                                                                                                     <  1:279822/140‑1 (MQ=255)                        aCCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCa                                                                                                     <  2:940158/140‑1 (MQ=255)                        aCCTTTGAGATAGATAACGCCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCa                                                                                                     <  2:320918/140‑1 (MQ=255)                            ttGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGt                                                                                                 >  2:1335712/1‑140 (MQ=255)                              gagaTAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCa                                                                                                     <  2:474150/134‑1 (MQ=255)                              gagaTAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCa                                                                                                     >  1:474150/1‑134 (MQ=255)                              gagaTAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGc                                                                                               >  2:381864/1‑140 (MQ=255)                              gagaTAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGc                                                                                               >  1:970953/1‑140 (MQ=255)                              gagaTAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGc                                                                                               >  2:741470/1‑140 (MQ=255)                                    gataACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAAtt                                                                                         >  1:54500/1‑140 (MQ=255)                                    gataACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAAtt                                                                                         >  1:106975/1‑140 (MQ=255)                                       aaCGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAg                                                                                      >  2:625100/1‑140 (MQ=255)                                         cGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCg                                                                                    <  2:981604/140‑1 (MQ=255)                                         cGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCg                                                                                    <  2:615222/140‑1 (MQ=255)                                            ccTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTg                                                                                 >  2:769389/1‑140 (MQ=255)                                             cTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGa                                                                                <  1:381864/140‑1 (MQ=255)                                              tCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGaa                                                                               >  1:1294028/1‑140 (MQ=255)                                              tCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGaa                                                                               >  1:531031/1‑140 (MQ=255)                                               cAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAg                                                                              >  1:899763/1‑140 (MQ=255)                                                      tCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACgg                                                                       >  1:1399771/1‑140 (MQ=255)                                                      tCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACgg                                                                       >  1:766532/1‑140 (MQ=255)                                                      tCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACACCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACgg                                                                       >  2:1429892/1‑140 (MQ=255)                                                       cTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGa                                                                      <  2:54500/140‑1 (MQ=255)                                                       cTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGa                                                                      <  2:1294028/140‑1 (MQ=255)                                                              cGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCa                                                               >  2:471508/1‑140 (MQ=255)                                                              cGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCa                                                               <  2:1156048/140‑1 (MQ=255)                                                                 aGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTg                                                            <  2:899763/140‑1 (MQ=255)                                                                   aTCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCa                                                          <  1:296313/140‑1 (MQ=255)                                                                     cAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCAcc                                                        <  1:576137/140‑1 (MQ=255)                                                                     cAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCAcc                                                        <  1:1293066/140‑1 (MQ=255)                                                                     cAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCAcc                                                        <  1:625100/140‑1 (MQ=255)                                                                     cAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCAcc                                                        <  2:225126/140‑1 (MQ=255)                                                                           cGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCa                                                  <  1:187132/140‑1 (MQ=255)                                                                             cAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGa                                                     >  1:283222/1‑135 (MQ=255)                                                                             cAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGa                                                     <  2:283222/135‑1 (MQ=255)                                                                               gTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATAtt                                              <  2:940485/140‑1 (MQ=255)                                                                                tGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCa                                                          <  2:590401/127‑1 (MQ=255)                                                                                tGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCa                                                          >  1:590401/1‑127 (MQ=255)                                                                                          aaCCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGAc                                   <  1:471508/140‑1 (MQ=255)                                                                                            ccTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCg                                 <  1:769389/140‑1 (MQ=255)                                                                                              tGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAg                               <  1:1456871/140‑1 (MQ=255)                                                                                              tGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAg                               <  1:396772/140‑1 (MQ=255)                                                                                              tGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATAACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAg                               <  2:927329/140‑1 (MQ=255)                                                                                                gTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCg                             >  2:866481/1‑140 (MQ=255)                                                                                                gTCTATAACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCg                             >  1:476975/1‑140 (MQ=255)                                                                                                     taACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAg                               >  1:364504/1‑133 (MQ=255)                                                                                                     taACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAg                               <  2:364504/133‑1 (MQ=255)                                                                                                     taACGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGttttt                        >  1:1453515/1‑140 (MQ=255)                                                                                                      aaCGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTa                       <  2:106975/140‑1 (MQ=255)                                                                                                       aCGCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTAt                      <  2:476975/140‑1 (MQ=255)                                                                                                         gCCGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATcg                    <  2:766532/140‑1 (MQ=255)                                                                                                          ccGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGTCTGACCGAGCGTTTTTATcgc                   >  1:1061587/1‑140 (MQ=255)                                                                                                          ccGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGCGCGTTTTTATcgc                   >  1:649333/1‑140 (MQ=255)                                                                                                          ccGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATcgc                   <  2:163506/140‑1 (MQ=255)                                                                                                          ccGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATcgc                   >  1:451308/1‑140 (MQ=255)                                                                                                           cGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATcgcg                  >  2:24449/1‑140 (MQ=255)                                                                                                           cGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATcgcg                  >  2:498573/1‑140 (MQ=255)                                                                                                           cGTGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATcgcg                  >  1:995033/1‑140 (MQ=255)                                                                                                             tGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTAt                      <  2:637464/134‑1 (MQ=255)                                                                                                             tGAATCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTAt                      >  1:637464/1‑134 (MQ=255)                                                                                                               aaccATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGa              >  2:386984/4‑140 (MQ=255)                                                                                                               aaTCATACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGa              >  1:224919/1‑140 (MQ=255)                                                                                                                    tACGCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAg         >  1:528795/1‑140 (MQ=255)                                                                                                                       gCCGGAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAgcgc      >  1:705271/1‑140 (MQ=255)                                                                                                                           gAAGGCACGTATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTc  >  2:181813/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                   TGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTC  >  NC_000913/418769‑419030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑