Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,280,182	T→C	C423C (TGT→TGC)	kbaZ →	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,280,182	0	T	C	100.0%	239.5 / NA	64	C423C (TGT→TGC)	kbaZ	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/27);  total (37/27)

CCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGC  >  NC_000913/3280051‑3280317                                                                                                                                    |                                                                                                                                        ccgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCa                                                                                                                                 >  2:1207478/1‑140 (MQ=255)    ctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATa                                                                                                                              >  2:32486/1‑140 (MQ=255)      gccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAg                                                                                                                            <  1:680187/140‑1 (MQ=255)          ctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCaaa                                                                                                                        >  2:494753/1‑140 (MQ=255)               cAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACaaa                                                                                                                   <  1:94916/140‑1 (MQ=255)               cAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACaaa                                                                                                                   <  2:361133/140‑1 (MQ=255)                     gTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGaa                                                                                                             <  1:1081287/140‑1 (MQ=255)                         cTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACg                                                                                                         <  1:247308/140‑1 (MQ=255)                                tGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGcc                                                                                                  <  1:496856/140‑1 (MQ=255)                                       cGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATAtt                                                                                           >  1:538945/1‑140 (MQ=255)                                         tGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGt                                                                                         >  2:19233/1‑140 (MQ=255)                                          gAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGtt                                                                                        <  2:538945/140‑1 (MQ=255)                                               ttCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAg                                                                                   <  1:1061429/140‑1 (MQ=255)                                                tCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGc                                                                                  <  2:1424098/140‑1 (MQ=255)                                                 cGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGcc                                                                                 >  1:701636/1‑140 (MQ=255)                                                 cGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGcc                                                                                 >  2:747393/1‑140 (MQ=255)                                                 cGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGcc                                                                                 >  2:1093267/1‑140 (MQ=255)                                                 cGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGcc                                                                                 >  1:755689/1‑140 (MQ=255)                                                        gCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtga                                                                          >  2:504864/1‑140 (MQ=255)                                                        gCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtga                                                                          >  2:620366/1‑140 (MQ=255)                                                         cGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtgat                                                                         >  1:359850/1‑140 (MQ=255)                                                          gAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtgatg                                                                        <  1:980644/140‑1 (MQ=255)                                                            gCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGaa                                                                      <  2:359850/140‑1 (MQ=255)                                                                cAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCt                                                                  <  2:755689/140‑1 (MQ=255)                                                                     aaCGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAtt                                                             <  1:564933/140‑1 (MQ=255)                                                                          cACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACgg                                                        <  1:504864/140‑1 (MQ=255)                                                                             gggAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCAGATTGATGAACGCTTGATTCACGGTCa                                                     >  2:617912/1‑140 (MQ=255)                                                                              ggAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAg                                                    >  1:1421136/1‑140 (MQ=255)                                                                                  cTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCg                                                >  1:1308075/1‑140 (MQ=255)                                                                                  cTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCg                                                <  2:1308075/140‑1 (MQ=255)                                                                                   tCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCgg                                               <  1:32486/140‑1 (MQ=255)                                                                                       tATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGTCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGtt                                           >  2:247585/1‑140 (MQ=255)                                                                                       tATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGtt                                           >  1:1377723/1‑140 (MQ=255)                                                                                         tCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCa                                         >  1:1165785/1‑140 (MQ=255)                                                                                            aCCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATg                                      >  1:1158415/1‑140 (MQ=255)                                                                                                       gACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTg                           >  1:879774/1‑140 (MQ=255)                                                                                                       gACATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTg                           >  1:1185519/1‑140 (MQ=255)                                                                                                        aCATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGc                          <  2:879774/140‑1 (MQ=255)                                                                                                        aCATCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGc                          <  1:111464/140‑1 (MQ=255)                                                                                                          aTCCTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCgg                        >  2:764300/1‑140 (MQ=255)                                                                                                            ccTGGCGCAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCgggg                      >  1:932582/1‑140 (MQ=255)                                                                                                                gcgcAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCaa                  <  2:1165785/140‑1 (MQ=255)                                                                                                                gcgcAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCaa                  <  1:1093267/140‑1 (MQ=255)                                                                                                                gcgaAGTACCACCCAGCCTGGGAAGGCCATTAAGCAAAACAAAGAGGATCAAGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCaa                  <  1:980316/140‑1 (MQ=255)                                                                                                                  gcAGTACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAAt                <  1:494753/140‑1 (MQ=255)                                                                                                                      tACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCaa                  >  1:579791/1‑134 (MQ=255)                                                                                                                      tACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCaa                  <  2:579791/134‑1 (MQ=255)                                                                                                                      tACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTgg            >  1:614699/1‑140 (MQ=255)                                                                                                                      tACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTgg            >  1:78224/1‑140 (MQ=255)                                                                                                                      tACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTgg            <  2:701636/140‑1 (MQ=255)                                                                                                                      tACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTgg            >  2:560004/1‑140 (MQ=255)                                                                                                                      tACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTgg            >  1:1410341/1‑140 (MQ=255)                                                                                                                      tACCACACAGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTgg            >  1:275802/1‑140 (MQ=255)                                                                                                                         cacacaGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGc         <  1:764300/140‑1 (MQ=255)                                                                                                                            acaGCCTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTgg      <  1:14577/140‑1 (MQ=255)                                                                                                                             caGCCTGCGAAGGCCAATAAGCAAAACAAA‑‑‑GAACACGCTATGCAAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGc  <  1:1207478/140‑1 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGc                    >  2:694990/1‑122 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGc                    <  1:694990/122‑1 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGc  >  2:366619/1‑140 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGc  >  1:414566/1‑140 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGc  >  1:497685/1‑140 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGc  >  2:1316863/1‑140 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGc  >  2:261226/1‑140 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGc  >  1:660067/1‑140 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGc  >  2:855926/1‑140 (MQ=255)                                                                                                                                ccTGCGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGc  >  1:922907/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        CCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGC  >  NC_000913/3280051‑3280317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑