Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,441,085	T→C	E197E (GAA→GAG)	rpsD ←	30S ribosomal subunit protein S4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,441,085	0	T	C	100.0%	279.8 / NA	75	E197E (GAA→GAG)	rpsD	30S ribosomal subunit protein S4
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/38);  total (37/38)

CACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCACGCGAGAC  >  NC_000913/3440952‑3441220                                                                                                                                        |                                                                                                                                        cACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCC‑CTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTaa                                                                                                                                  <  2:728484/140‑1 (MQ=255)       ggCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTcc                                                                                                                             >  1:1196014/1‑140 (MQ=255)          gtgcgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGca                                                                                                                          <  2:734348/140‑1 (MQ=255)          gtgcgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGca                                                                                                                          <  2:716163/140‑1 (MQ=255)            gcgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGcaga                                                                                                                        <  2:1168884/140‑1 (MQ=255)            gcgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGcaga                                                                                                                        <  2:1268707/140‑1 (MQ=255)            gcgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGcaga                                                                                                                        <  1:550363/140‑1 (MQ=255)             cgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGcagac                                                                                                                       >  1:1057109/1‑140 (MQ=255)             cgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGcagac                                                                                                                       >  2:1363915/1‑140 (MQ=255)             cgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGcaga                                                                                                                        <  2:1304883/139‑1 (MQ=255)             cgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGcaga                                                                                                                        >  1:1304883/1‑139 (MQ=255)                 gAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGAt                                                                                                                   <  2:1196014/140‑1 (MQ=255)                  aaCTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATc                                                                                                                  >  1:446200/1‑140 (MQ=255)                  aaCTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATc                                                                                                                  >  1:624010/1‑140 (MQ=255)                     tCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGa                                                                                                               <  1:142541/140‑1 (MQ=255)                                atatCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCt                                                                                                    <  2:488697/140‑1 (MQ=255)                                 tatCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCtt                                                                                                   <  2:446200/140‑1 (MQ=255)                                 tatCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCtt                                                                                                   >  2:1363758/1‑140 (MQ=255)                                 tatCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCtt                                                                                                   <  2:83676/140‑1 (MQ=255)                                  atCAACCAGGCGCGGTTTTAGAAACTCTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCt                                                                                                    <  1:673116/140‑1 (MQ=255)                                    cAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACg                                                                                                <  2:332953/140‑1 (MQ=255)                                    cAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACg                                                                                                <  2:33226/140‑1 (MQ=255)                                      aCCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCt                                                                                              >  2:587216/1‑140 (MQ=255)                                           gcgcGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAc                                                                                         >  2:410950/1‑140 (MQ=255)                                           gcgcGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAc                                                                                         >  1:733020/1‑140 (MQ=255)                                            cgcgGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACg                                                                                        <  1:451301/140‑1 (MQ=255)                                            cgcgGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACg                                                                                        <  2:847332/140‑1 (MQ=255)                                            cgcgGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACg                                                                                        <  2:379967/140‑1 (MQ=255)                                                 ttttAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCt                                                                                   <  2:624010/140‑1 (MQ=255)                                                   ttAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTc                                                                                 <  2:897018/140‑1 (MQ=255)                                                      gAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCAt                                                                              <  1:490772/140‑1 (MQ=255)                                                         a‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCtt                                                                           >  2:656479/1‑140 (MQ=255)                                                             tctGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGc                                                                         >  1:238247/1‑140 (MQ=255)                                                              ctGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGcc                                                                        <  2:364448/140‑1 (MQ=255)                                                               tGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCa                                                                       >  1:860136/1‑140 (MQ=255)                                                               tGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCa                                                                       >  2:399265/1‑140 (MQ=255)                                                                     aGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCa                                                                 <  1:1363915/140‑1 (MQ=255)                                                                           cTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTcc                                                           >  1:1283089/1‑140 (MQ=255)                                                                            tGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTcca                                                          >  2:150461/1‑140 (MQ=255)                                                                                 tgtgTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTccagccag                                                     <  2:1057109/140‑1 (MQ=255)                                                                                  gtgtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTccagccagt                                                    <  2:1283089/140‑2 (MQ=255)                                                                                  gtgtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAgg                                                    <  1:212506/140‑1 (MQ=255)                                                                                  gtgtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAgg                                                    >  1:220909/1‑140 (MQ=255)                                                                                   tgtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGt                                                   >  2:1214193/1‑140 (MQ=255)                                                                                   tgtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGt                                                   >  1:972402/1‑140 (MQ=255)                                                                                        tctctTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCt                                              >  2:743842/1‑140 (MQ=255)                                                                                        tctctTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCt                                              >  2:1072185/1‑140 (MQ=255)                                                                                           ctTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCtttt                                           >  1:796479/1‑140 (MQ=255)                                                                                              tGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCAc                                        <  1:765803/140‑1 (MQ=255)                                                                                                  aCTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACgctg                                    >  2:1422681/1‑140 (MQ=255)                                                                                                    tAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTg                                                 >  2:1100159/1‑125 (MQ=255)                                                                                                    tAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTg                                                 <  1:1100159/125‑1 (MQ=255)                                                                                                    tAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACgctgct                                  >  2:1222427/1‑140 (MQ=255)                                                                                                      aGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTca                                >  2:1359322/1‑140 (MQ=255)                                                                                                      aGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTca                                >  2:738576/1‑140 (MQ=255)                                                                                                      aGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTca                                <  2:772119/140‑1 (MQ=255)                                                                                                      aGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTca                                >  1:772119/1‑140 (MQ=255)                                                                                                        cTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagc                              <  1:1072185/140‑1 (MQ=255)                                                                                                        cTTTACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagc                              <  1:150461/140‑1 (MQ=255)                                                                                                           tACTTGGAGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagccag                           >  2:872290/1‑140 (MQ=255)                                                                                                                  aGTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCaga                    >  1:1082578/1‑140 (MQ=255)                                                                                                                   gTAAAGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCagag                   <  2:1082578/140‑1 (MQ=255)                                                                                                                     aaaGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCg                 <  1:1214193/140‑1 (MQ=255)                                                                                                                      aaGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCgg                >  2:433942/1‑140 (MQ=255)                                                                                                                       aGCTCGACGATCAGGTGCTCGTTAATGTCCGCAGACACATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGc               >  2:295022/1‑140 (MQ=255)                                                                                                                          tCGACGATCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCttt            <  2:352438/140‑1 (MQ=255)                                                                                                                            gacgaTCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCa          >  1:1276286/1‑140 (MQ=255)                                                                                                                            gacgaTCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCa          >  1:1130639/1‑140 (MQ=255)                                                                                                                            gacgaTCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCa          >  2:1008575/1‑140 (MQ=255)                                                                                                                              cgaTCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTTCCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCAcg        <  2:1276286/140‑1 (MQ=255)                                                                                                                               gaTCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTAGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCAcgc       <  1:1422681/140‑1 (MQ=255)                                                                                                                                 tCAGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCACGCga     >  1:488388/1‑140 (MQ=255)                                                                                                                                   aGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCACGCgaga   >  2:426118/1‑140 (MQ=255)                                                                                                                                   aGGTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCACGCgaga   <  2:299769/140‑1 (MQ=255)                                                                                                                                    ggTGCTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCACGCGAGAc  <  1:433942/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAA‑‑CTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTTTCACGCGAGAC  >  NC_000913/3440952‑3441220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑