Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,913,539	T→C	T44A (ACC→GCC)	glmS ←	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,913,539	0	T	C	100.0%	314.3 / NA	86	T44A (ACC→GCC)	glmS	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/46);  total (40/46)

TGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATA  >  NC_000913/3913403‑3913669                                                                                                                                         |                                                                                                                                   tGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCaa                                                                                                                                 >  1:366574/1‑139 (MQ=255)    ggATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATg                                                                                                                              <  1:139129/140‑1 (MQ=255)    ggATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATg                                                                                                                              <  2:752065/140‑1 (MQ=255)               cACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCAt                                                                                                                   <  1:768652/140‑1 (MQ=255)                         ggTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGc                                                                                                         <  2:1380971/140‑1 (MQ=255)                            tCACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAg                                                                                                      >  1:1253870/1‑140 (MQ=255)                             cACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGa                                                                                                     >  2:445876/1‑140 (MQ=255)                             cACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGa                                                                                                     <  1:640987/140‑1 (MQ=255)                              aCCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGAc                                                                                                    >  2:5555/1‑140 (MQ=255)                              aCCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGAc                                                                                                    >  1:1205732/1‑140 (MQ=255)                              aCCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGAc                                                                                                    >  1:689348/1‑140 (MQ=255)                              aCCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGAc                                                                                                    >  1:620812/1‑140 (MQ=255)                              aCCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGAc                                                                                                    >  1:61672/1‑140 (MQ=255)                               ccGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGAcc                                                                                                   <  2:689348/140‑1 (MQ=255)                                  tGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGc                                                                                                <  1:5555/140‑1 (MQ=255)                                   gggTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCa                                                                                               <  1:858760/140‑1 (MQ=255)                                   gggTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCa                                                                                               >  1:179966/1‑140 (MQ=255)                                   gggTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCa                                                                                               >  2:667068/1‑140 (MQ=255)                                     gTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCaga                                                                                             <  1:1068050/140‑1 (MQ=255)                                         cccAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCa                                                                                         >  1:92120/1‑140 (MQ=255)                                         cccAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCa                                                                                         >  2:768253/1‑140 (MQ=255)                                         cccAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCa                                                                                         >  2:831196/1‑140 (MQ=255)                                          ccAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGc                                                                                                >  1:254076/1‑132 (MQ=255)                                          ccAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGc                                                                                                <  2:254076/132‑1 (MQ=255)                                          ccAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCat                                                                                        >  2:541724/1‑140 (MQ=255)                                          ccAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCat                                                                                        >  2:186252/1‑140 (MQ=255)                                          ccAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCat                                                                                        <  1:606675/140‑1 (MQ=255)                                             gCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATc                                                                                     <  2:1253870/140‑1 (MQ=255)                                             gCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATc                                                                                     <  2:1423512/140‑1 (MQ=255)                                               gAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGATTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCcg                                                                                   <  2:1129134/140‑1 (MQ=255)                                                 gtgtGAGCAATCCCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCcgcg                                                                                 <  1:1327185/140‑1 (MQ=255)                                                    tgAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCcgcg                                                                                 >  1:681977/1‑137 (MQ=255)                                                    tgAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCcgcg                                                                                 <  2:681977/137‑1 (MQ=255)                                                     gAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTAt                                                                             <  2:899971/140‑1 (MQ=255)                                                      aGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTAtt                                                                            >  1:140904/1‑140 (MQ=255)                                                        cAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTcc                                                                          >  2:1236198/1‑140 (MQ=255)                                                          aTACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAg                                                                        <  2:61672/140‑1 (MQ=255)                                                          aTACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAg                                                                        <  1:667068/140‑1 (MQ=255)                                                           tACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAga                                                                       >  2:571285/1‑140 (MQ=255)                                                           tACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAga                                                                       <  2:179966/140‑1 (MQ=255)                                                           tACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAga                                                                       <  1:243121/140‑1 (MQ=255)                                                           tACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAga                                                                       <  1:1455951/140‑1 (MQ=255)                                                           tACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAga                                                                       <  1:1236198/140‑1 (MQ=255)                                                            aCCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAgac                                                                      >  2:95695/1‑140 (MQ=255)                                                                gTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGacgacg                                                                  >  2:618792/1‑140 (MQ=255)                                                                 tGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGt                                                                 <  1:3149/140‑1 (MQ=255)                                                                  gccgccATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTa                                                                <  1:541724/140‑1 (MQ=255)                                                                  gccgccATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTa                                                                >  2:326320/1‑140 (MQ=255)                                                                        aTGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCtt                                                          >  1:1220034/1‑140 (MQ=255)                                                                          gCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCa                                                        <  1:390017/140‑1 (MQ=255)                                                                             gagGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCaaga                                                     <  1:571285/140‑1 (MQ=255)                                                                             gagGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCaaga                                                     <  2:1034391/140‑1 (MQ=255)                                                                                gATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAgg                                                  >  2:906854/1‑140 (MQ=255)                                                                                  tGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGAt                                                <  2:92120/140‑1 (MQ=255)                                                                                   gTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTTAAGAAGGAtt                                               >  2:324916/1‑140 (MQ=255)                                                                                   gTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGAtt                                               >  1:1275302/1‑140 (MQ=255)                                                                                       ttcCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTg                                           <  2:771824/140‑1 (MQ=255)                                                                                       ttcCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTg                                           <  1:906854/140‑1 (MQ=255)                                                                                       ttcCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTg                                           <  1:326320/140‑1 (MQ=255)                                                                                       ttcCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTg                                           <  1:45568/140‑1 (MQ=255)                                                                                       ttcCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTg                                           <  1:831196/140‑1 (MQ=255)                                                                                         ccGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCt                                         <  1:768253/140‑1 (MQ=255)                                                                                          cGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTa                                        >  2:756359/1‑140 (MQ=255)                                                                                           gCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTAc                                       >  1:1048021/1‑140 (MQ=255)                                                                                                 tgtgCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACg                                 <  2:140904/140‑1 (MQ=255)                                                                                                     ccAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTgc                             >  2:898354/1‑140 (MQ=255)                                                                                                         cATCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGa                         >  1:905815/1‑140 (MQ=255)                                                                                                          aTCTGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGAt                        <  1:95695/140‑1 (MQ=255)                                                                                                             tGGACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATcgc                     >  2:614704/1‑140 (MQ=255)                                                                                                               gACTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAAACTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCgcgc                   >  2:871012/1‑140 (MQ=255)                                                                                                                aCTTTACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGcc                  <  2:114827/140‑1 (MQ=255)                                                                                                                  tttACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCcaa                <  1:186252/140‑1 (MQ=255)                                                                                                                   ttACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCcaac               >  1:1376078/1‑140 (MQ=255)                                                                                                                    tACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCcaaca              >  2:1445681/1‑140 (MQ=255)                                                                                                                    tACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCcaaca              <  2:78194/140‑1 (MQ=255)                                                                                                                    tACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCcaaca              >  1:697507/1‑140 (MQ=255)                                                                                                                    tACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCcaaca              >  2:467551/1‑140 (MQ=255)                                                                                                                    tACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCcaa                <  2:1024852/138‑1 (MQ=255)                                                                                                                    tACCGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCcaa                >  1:1024852/1‑138 (MQ=255)                                                                                                                      ccGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAAt            <  2:673160/140‑1 (MQ=255)                                                                                                                      ccGAGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCAAACAAt            <  1:756359/140‑1 (MQ=255)                                                                                                                         aGGCGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTcc         >  1:279843/1‑140 (MQ=255)                                                                                                                            cGACGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCcaca      <  2:1376078/140‑1 (MQ=255)                                                                                                                              aCGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCcacaca    <  2:76357/140‑1 (MQ=255)                                                                                                                              aCGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCcacaca    <  1:1445681/140‑1 (MQ=255)                                                                                                                              aCGCAGGCGGGCCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCcacaca    >  1:1206598/1‑140 (MQ=255)                                                                                                                                gCAGGCGGGCCATATGTCCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCCCACATa  <  1:871012/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATA  >  NC_000913/3913403‑3913669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑