Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,477	C→T	P442L (CCC→CTC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,477	0	C	T	100.0%	331.7 / NA	95	P442L (CCC→CTC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/52);  total (43/52)

ATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTAC  >  NC_000913/3992344‑3992613                                                                                                                                      |                                                                                                                                         aTCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtct                                                                                                                                    >  1:1022868/1‑140 (MQ=255) aTCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtct                                                                                                                                    >  2:1140150/1‑140 (MQ=255) aTCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtct                                                                                                                                    >  1:520187/1‑140 (MQ=255) aTCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtct                                                                                                                                    >  2:1114825/1‑140 (MQ=255) aTCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtct                                                                                                                                    >  1:864486/1‑140 (MQ=255) aTCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtct                                                                                                                                    >  1:259922/1‑140 (MQ=255) aTCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtct                                                                                                                                    >  1:190719/1‑140 (MQ=255) aTCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtct                                                                                                                                    >  1:980396/1‑140 (MQ=255)  tCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtctc                                                                                                                                   >  1:508438/1‑140 (MQ=255)   ccGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGAtctct                                                                                                                                  <  1:138896/140‑1 (MQ=255)      cTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCgg                                                                                                                               >  2:332983/1‑140 (MQ=255)      cTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCgg                                                                                                                               >  2:1040223/1‑140 (MQ=255)      cTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCgg                                                                                                                               <  2:864486/140‑1 (MQ=255)         tGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAAc                                                                                                                            >  1:986553/1‑140 (MQ=255)           cgcgTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCg                                                                                                                          >  2:461055/1‑140 (MQ=255)             cgTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGaa                                                                                                                        <  1:332983/140‑1 (MQ=255)               tCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATc                                                                                                                      <  2:644882/140‑1 (MQ=255)                cGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCt                                                                                                                     <  2:613455/140‑1 (MQ=255)                 gCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTg                                                                                                                    <  1:245806/140‑1 (MQ=255)                 gCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTg                                                                                                                    <  1:1140150/140‑1 (MQ=255)                 gCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTg                                                                                                                    <  2:534153/140‑1 (MQ=255)                 gCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTg                                                                                                                    <  2:1372047/140‑1 (MQ=255)                  cAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGa                                                                                                                   <  1:702333/140‑1 (MQ=255)                   aaTAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGAc                                                                                                                  <  2:1060291/140‑1 (MQ=255)                   aaTAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGAc                                                                                                                  <  1:638811/140‑1 (MQ=255)                           tAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCtttattt                                                                                                          <  1:690603/140‑1 (MQ=255)                            aGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAAt                                                                                                                       <  2:497634/126‑1 (MQ=255)                            aGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAAt                                                                                                                       >  1:497634/1‑126 (MQ=255)                                tctcCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTAtgtg                                                                                                     >  2:1339546/1‑140 (MQ=255)                                  tcCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTgcc                                                                                                   >  1:443043/1‑140 (MQ=255)                                   ccGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTgccg                                                                                                  >  2:1290976/1‑140 (MQ=255)                                    cGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTgccgc                                                                                                 <  2:624496/140‑1 (MQ=255)                                                    atCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCg                                                                                 >  2:517358/1‑140 (MQ=255)                                                       ggCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTc                                                                              <  1:1339546/140‑1 (MQ=255)                                                       ggCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTc                                                                              <  1:1290976/140‑1 (MQ=255)                                                        gCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCa                                                                             >  1:470723/1‑140 (MQ=255)                                                         cGTGCTGACACGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAg                                                                            <  1:974710/140‑1 (MQ=255)                                                           tGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGt                                                                          >  1:834227/1‑140 (MQ=255)                                                           tGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGt                                                                          <  1:333426/140‑1 (MQ=255)                                                           tGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGt                                                                          >  2:1039696/1‑140 (MQ=255)                                                             cTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAgg                                                                           >  2:364480/1‑137 (MQ=255)                                                             cTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAgg                                                                           <  1:364480/137‑1 (MQ=255)                                                             cTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTg                                                                        >  1:575431/1‑140 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGTAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  2:1377270/140‑1 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  2:986553/140‑1 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  2:470723/140‑1 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  2:575431/140‑1 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  2:795115/140‑1 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  2:875398/140‑1 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  1:1040223/140‑1 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  1:1114825/140‑1 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  1:461055/140‑1 (MQ=255)                                                               gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  1:517358/140‑1 (MQ=255)                                                                aCGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTa                                                                     >  2:571706/1‑140 (MQ=255)                                                                 cgcgTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTAt                                                                    <  2:1031874/140‑1 (MQ=255)                                                                 cgcgTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTAt                                                                    <  1:1039696/140‑1 (MQ=255)                                                                   cgTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCt                                                                  <  2:259922/140‑1 (MQ=255)                                                                     tAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      >  2:1196335/1‑134 (MQ=255)                                                                     tAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                      <  1:1196335/134‑1 (MQ=255)                                                                            tATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAgg                                                                           >  2:622609/1‑122 (MQ=255)                                                                            tATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAgg                                                                           <  1:622609/122‑1 (MQ=255)                                                                            tATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcg                                                         >  1:958152/1‑140 (MQ=255)                                                                            tATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcg                                                         >  2:477434/1‑140 (MQ=255)                                                                            tATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTCCAGGTTGGTATCTGTATAACcg                                                         >  1:686257/1‑140 (MQ=255)                                                                             aTGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcgc                                                        <  2:190719/140‑1 (MQ=255)                                                                               gCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcgcgc                                                      <  1:1185398/140‑1 (MQ=255)                                                                                 cgcgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCgcgcgc                                                    >  2:77678/1‑140 (MQ=255)                                                                                  gcgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGcc                                                   <  2:443043/140‑1 (MQ=255)                                                                                  gcgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGcc                                                   <  2:958152/140‑1 (MQ=255)                                                                                  gcgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGcc                                                   >  1:220801/1‑140 (MQ=255)                                                                                   cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGt                                                                <  1:590743/126‑1 (MQ=255)                                                                                   cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGt                                                                >  2:590743/1‑126 (MQ=255)                                                                                   cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcg                                                         <  1:862077/133‑1 (MQ=255)                                                                                   cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcg                                                         >  2:862077/1‑133 (MQ=255)                                                                                   cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGcc                                                   >  1:613718/1‑139 (MQ=255)                                                                                   cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGcc                                                   <  2:613718/139‑1 (MQ=255)                                                                                   cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCa                                                  >  1:853331/1‑140 (MQ=255)                                                                                      ttGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAat                                               >  2:1342338/1‑140 (MQ=255)                                                                                         aaGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATAtt                                            <  1:477434/140‑1 (MQ=255)                                                                                          aGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTg                                           <  2:508438/140‑1 (MQ=255)                                                                                             aTTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGt                                        <  1:1342338/140‑1 (MQ=255)                                                                                                aCCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGcc                                                   <  1:510584/126‑1 (MQ=255)                                                                                                aCCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGcc                                                   >  2:510584/1‑126 (MQ=255)                                                                                                aCCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGa                                     >  1:1371715/1‑140 (MQ=255)                                                                                                    ggTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGatcat                                 <  2:953339/140‑1 (MQ=255)                                                                                                      tAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGAtcatca                               >  2:955407/1‑140 (MQ=255)                                                                                                            gACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatc                         <  2:1371715/140‑1 (MQ=255)                                                                                                                  ggTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGc                   <  2:834227/140‑1 (MQ=255)                                                                                                                   gTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCt                  <  1:635005/140‑1 (MQ=255)                                                                                                                   gTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCt                  <  1:1367281/140‑1 (MQ=255)                                                                                                                    tAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatc                         <  1:1246618/132‑1 (MQ=255)                                                                                                                    tAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatc                         >  2:1246618/1‑132 (MQ=255)                                                                                                                             aGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAAc        >  2:927637/1‑140 (MQ=255)                                                                                                                             aGATTTCACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGCATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAAc        >  1:164525/1‑140 (MQ=255)                                                                                                                                   cACTCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTAc  >  2:48391/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         ATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTAC  >  NC_000913/3992344‑3992613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑