Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,055,309 T→C Y344C (TAC→TGC)  glnL ← sensory histidine kinase NtrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,055,3090TC100.0% 370.5 / NA 99Y344C (TAC→TGC) glnLsensory histidine kinase NtrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/49);  total (50/49)

TTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCG  >  NC_000913/4055175‑4055444
                                                                                                                                      |                                                                                                                                       
ttCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAAcc                                                                                                                                    <  1:238756/140‑1 (MQ=255)
ttCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAAcc                                                                                                                                    >  1:1440355/1‑140 (MQ=255)
     aaccgtcgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGaa                                                                                                                               >  1:1200185/4‑140 (MQ=255)
     aaaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGaa                                                                                                                               <  2:1423161/140‑1 (MQ=255)
     aaaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGaa                                                                                                                               <  1:1127708/140‑1 (MQ=255)
      accgtcgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAAc                                                                                                                              <  2:1200185/138‑1 (MQ=255)
       aCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAGGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACt                                                                                                                             <  2:249699/140‑1 (MQ=255)
           cgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGt                                                                                                                         <  2:302639/140‑1 (MQ=255)
           cgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGt                                                                                                                         <  1:532973/140‑1 (MQ=255)
           cgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGt                                                                                                                         <  2:1440355/140‑1 (MQ=255)
             tACAGGTTAAACCTGCCCCAGCGAGCGCACGTTGAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTAt                                                                                                                       >  1:97366/1‑140 (MQ=255)
             tACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTAt                                                                                                                       >  1:459815/1‑140 (MQ=255)
             tACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTAt                                                                                                                       >  2:248162/1‑140 (MQ=255)
                aGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTGCTGATAGGCAGGCAAACCGAGAACTCGGTATGcc                                                                                                                    <  2:349457/140‑1 (MQ=255)
                     aaaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGc                                                                                                               <  1:252621/140‑1 (MQ=255)
                       aCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGt                                                                                                                         <  1:1294449/128‑1 (MQ=255)
                       aCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGt                                                                                                                         >  2:1294449/1‑128 (MQ=255)
                       aCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCa                                                                                                             >  1:193192/1‑140 (MQ=255)
                            ccccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGt                                                                                                                         <  1:798595/123‑1 (MQ=255)
                            ccccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGt                                                                                                                         >  2:798595/1‑123 (MQ=255)
                            ccccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTgg                                                                                                        >  2:952870/1‑140 (MQ=255)
                            ccccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTgg                                                                                                        >  2:1352728/1‑140 (MQ=255)
                            ccccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTgg                                                                                                        >  1:1168989/1‑140 (MQ=255)
                            ccccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTgg                                                                                                        >  2:1424460/1‑140 (MQ=255)
                            ccccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTgg                                                                                                        >  2:1174944/1‑140 (MQ=255)
                            ccccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTgg                                                                                                        >  1:1262134/1‑140 (MQ=255)
                             cccAGCAAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGt                                                                                                       <  1:1303535/140‑1 (MQ=255)
                                agcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaaa                                                                                                    >  1:1409040/1‑140 (MQ=255)
                                 gcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAt                                                                                                   <  1:1352728/140‑1 (MQ=255)
                                 gcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAt                                                                                                   <  1:664198/140‑1 (MQ=255)
                                  cgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAtt                                                                                                  >  1:69809/1‑140 (MQ=255)
                                      cgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAt                                                                                              >  1:76806/1‑140 (MQ=255)
                                      cgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAt                                                                                              >  1:579780/1‑140 (MQ=255)
                                        cACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAttt                                                                                            <  1:658150/140‑1 (MQ=255)
                                           gTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGc                                                                                         <  1:957356/140‑1 (MQ=255)
                                            ttCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGcc                                                                                        <  1:569594/140‑1 (MQ=255)
                                            ttCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGAATGCCCTCGCCAACTGGTAAATTCAAGTTTGcc                                                                                        >  2:1357753/1‑140 (MQ=255)
                                             tCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCt                                                                                       <  2:459815/140‑1 (MQ=255)
                                                  cACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATg                                                                                  <  1:504545/140‑1 (MQ=255)
                                                     ccAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTg                                                                               >  1:1285304/1‑140 (MQ=255)
                                                     ccAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTg                                                                               <  2:1168989/140‑1 (MQ=255)
                                                     ccAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTg                                                                               <  1:952870/140‑1 (MQ=255)
                                                       aaCGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGat                                                                             >  1:542372/1‑140 (MQ=255)
                                                       aaCGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGat                                                                             <  1:44423/140‑1 (MQ=255)
                                                       aaCGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGat                                                                             <  1:1424460/140‑1 (MQ=255)
                                                         cGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGatca                                                                           <  1:484255/140‑1 (MQ=255)
                                                                   tATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTAc                                                                 <  2:97366/140‑1 (MQ=255)
                                                                   tATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTAc                                                                 <  2:1409040/140‑1 (MQ=255)
                                                                      cGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAg                                                              <  1:858284/140‑1 (MQ=255)
                                                                        tCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCg                                                            <  1:236426/140‑1 (MQ=255)
                                                                          aTCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGAt                                                          >  1:678826/1‑140 (MQ=255)
                                                                          aTCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGAt                                                          >  1:902085/1‑140 (MQ=255)
                                                                          aTCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGAt                                                          >  1:219920/1‑140 (MQ=255)
                                                                             gacgacCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGa                                                       <  1:700938/140‑1 (MQ=255)
                                                                             gACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGa                                                       <  2:902085/140‑1 (MQ=255)
                                                                                  cccAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATaagc                                                  >  2:46615/1‑140 (MQ=255)
                                                                                  cccAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATaagc                                                  >  2:874436/1‑140 (MQ=255)
                                                                                    cAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTAATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATaagcca                                                >  2:839207/1‑140 (MQ=255)
                                                                                      gACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATaagccaag                                              >  1:898501/1‑140 (MQ=255)
                                                                                       aCTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATaagccaagc                                             >  2:1370469/1‑140 (MQ=255)
                                                                                       aCTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATaagccaagc                                             >  1:1070084/1‑140 (MQ=255)
                                                                                       aCTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATaagccaagc                                             >  2:1260742/1‑140 (MQ=255)
                                                                                       aCTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATaagccaagc                                             >  2:1395974/1‑140 (MQ=255)
                                                                                            cccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGAt                                                      >  1:1403928/1‑126 (MQ=255)
                                                                                            cccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGAt                                                      <  2:1403928/126‑1 (MQ=255)
                                                                                            cccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGt                                        >  1:1044297/1‑140 (MQ=255)
                                                                                            cccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGt                                        >  2:536843/1‑140 (MQ=255)
                                                                                            cccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGt                                        >  2:1244415/1‑140 (MQ=255)
                                                                                            cccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGt                                        >  1:987909/1‑140 (MQ=255)
                                                                                            cccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGt                                        >  1:262098/1‑140 (MQ=255)
                                                                                            cccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGt                                        >  1:956593/1‑140 (MQ=255)
                                                                                            cccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCCGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGt                                        >  2:381747/1‑140 (MQ=255)
                                                                                             ccTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTg                                       >  1:939355/1‑140 (MQ=255)
                                                                                              cTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGc                                      <  1:1395974/140‑1 (MQ=255)
                                                                                                   tGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCt                                 >  1:1007158/1‑140 (MQ=255)
                                                                                                   tGCATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCt                                 >  1:294781/1‑140 (MQ=255)
                                                                                                     cATAAACGTCACCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTc                               >  1:365598/1‑140 (MQ=255)
                                                                                                               aCCTTTATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTg                     >  1:339443/1‑140 (MQ=255)
                                                                                                                   ttATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 <  2:987909/140‑1 (MQ=255)
                                                                                                                   ttATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 <  2:69809/140‑1 (MQ=255)
                                                                                                                   ttATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 <  2:365598/140‑1 (MQ=255)
                                                                                                                   ttATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 <  2:339443/140‑1 (MQ=255)
                                                                                                                   ttATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 <  2:1070084/140‑1 (MQ=255)
                                                                                                                   ttATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 <  2:1044297/140‑1 (MQ=255)
                                                                                                                   ttATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 <  2:1007158/140‑1 (MQ=255)
                                                                                                                   ttATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 <  2:294781/140‑1 (MQ=255)
                                                                                                                    tATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 >  1:956534/1‑139 (MQ=255)
                                                                                                                    tATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCa                 <  2:956534/139‑1 (MQ=255)
                                                                                                                    tATTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCAt                >  2:897808/1‑140 (MQ=255)
                                                                                                                     aTTTCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATc               <  2:219920/140‑1 (MQ=255)
                                                                                                                        tCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCggg            <  2:898501/140‑1 (MQ=255)
                                                                                                                        tCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCggg            >  1:1084644/1‑140 (MQ=255)
                                                                                                                        tCCTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCggg            <  1:897808/140‑1 (MQ=255)
                                                                                                                          cTGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTa          <  2:678826/140‑1 (MQ=255)
                                                                                                                           tGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTaa         >  2:531575/1‑140 (MQ=255)
                                                                                                                           tGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTaa         >  2:215804/1‑140 (MQ=255)
                                                                                                                           tGATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTaa         >  1:962067/1‑140 (MQ=255)
                                                                                                                            gATAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTaaa        <  2:746736/140‑1 (MQ=255)
                                                                                                                             aTAGGCAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTaaaa       <  1:1049174/140‑1 (MQ=255)
                                                                                                                                  cAGGCAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCg  <  1:248162/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
TTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCG  >  NC_000913/4055175‑4055444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑