Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,247,001	C→A	A73E (GCA→GAA)	malK →	maltose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,247,001	0	C	A	100.0%	251.1 / NA	71	A73E (GCA→GAA)	malK	maltose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/45);  total (26/45)

TGAAGGTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGG  >  NC_000913/4246864‑4247135                                                                                                                                          |                                                                                                                                       tGAAGGTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCcagaag                                                                                                                                      >  1:70966/1‑140 (MQ=255) tGAAGGTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCAGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCcagaag                                                                                                                                      >  2:1094410/1‑140 (MQ=255)  gAAGGTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCagaaga                                                                                                                                     >  1:912375/1‑140 (MQ=255)     ggTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAAcg                                                                                                                                  <  1:1339167/140‑1 (MQ=255)     ggTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAAcg                                                                                                                                  <  2:1452251/140‑1 (MQ=255)     ggTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAAcg                                                                                                                                  >  1:207528/1‑140 (MQ=255)     ggTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAAcg                                                                                                                                  <  1:1444595/140‑1 (MQ=255)     ggTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAAcg                                                                                                                                  >  1:136116/1‑140 (MQ=255)     ggTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAAcg                                                                                                                                  <  1:779958/140‑1 (MQ=255)     ggTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAAcg                                                                                                                                  <  2:638499/140‑1 (MQ=255)       tGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAAcgcg                                                                                                                                >  2:807536/1‑140 (MQ=255)        gAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACgcgg                                                                                                                               <  1:1056093/140‑1 (MQ=255)         aaTTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACgcggc                                                                                                                              >  1:1267227/1‑140 (MQ=255)          aTTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGTTTGATCCCCTCGCAAGCGGCCACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACgcggcg                                                                                                                             <  2:1047693/140‑1 (MQ=255)          aTTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACgcggcg                                                                                                                             <  2:1267227/140‑1 (MQ=255)          aTTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAAAGACACTCCGCCAGAAGAACgcggcg                                                                                                                             <  2:1060937/140‑1 (MQ=255)                 gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGGCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTAt                                                                                                                      <  1:178848/140‑1 (MQ=255)                 gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTAt                                                                                                                      <  1:156901/140‑1 (MQ=255)                   gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCACCAGAAGAACGCGGCGTTGGTATgg                                                                                                                    <  1:1283086/140‑1 (MQ=255)                       gTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGtt                                                                                                                <  2:424572/140‑1 (MQ=255)                        tCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGtta                                                                                                               <  2:542824/140‑2 (MQ=255)                          ggACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCa                                                                                                             <  1:1409246/140‑1 (MQ=255)                            aCCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGt                                                                                                           <  1:807536/140‑1 (MQ=255)                              cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCt                                                                                                         >  2:737258/1‑140 (MQ=255)                                 cTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAc                                                                                                      <  1:496252/140‑1 (MQ=255)                                    gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcgcg                                                                                                   <  1:1094410/140‑1 (MQ=255)                                     cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACgcgc                                                                                                  <  2:207528/140‑1 (MQ=255)                                     cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCGGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACgcgc                                                                                                  <  2:762282/140‑1 (MQ=255)                                      tGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGct                                                                                                 <  1:424300/140‑1 (MQ=255)                                        cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                                                                                                     >  2:1278043/1‑134 (MQ=255)                                        cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                                                                                                     <  1:1278043/134‑1 (MQ=255)                                          gTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTAt                                                                                             >  2:202653/1‑140 (MQ=255)                                             aaTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATccc                                                                                          <  1:774777/140‑1 (MQ=255)                                             aaTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATccc                                                                                          <  1:335907/140‑1 (MQ=255)                                               tCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCa                                                                                        <  1:1284420/140‑1 (MQ=255)                                                  aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt                                                                                     >  2:297309/1‑140 (MQ=255)                                                       aCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAg                                                                                <  1:714230/140‑1 (MQ=255)                                                       aCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAg                                                                                >  2:594982/1‑140 (MQ=255)                                                           cgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGc                                                                            >  1:385665/1‑140 (MQ=255)                                                           cgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGc                                                                            <  1:349223/140‑1 (MQ=255)                                                             cATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAg                                                                          <  1:389594/140‑1 (MQ=255)                                                                    gCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACAt                                                                   <  1:737258/140‑1 (MQ=255)                                                                      cGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGt                                                                 >  1:691353/1‑140 (MQ=255)                                                                          cTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCAtt                                                             <  1:459390/140‑1 (MQ=255)                                                                                   aTCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGc                                                         <  1:834154/135‑1 (MQ=255)                                                                                   aTCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGc                                                         >  2:834154/1‑135 (MQ=255)                                                                                   aTCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGaa                                                    >  2:6756/1‑140 (MQ=255)                                                                                        cAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActgg                                               >  2:9688/1‑140 (MQ=255)                                                                                          gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggct                                             >  1:312484/1‑140 (MQ=255)                                                                                             gcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACtggctggc                                          >  2:272607/1‑140 (MQ=255)                                                                                                aCCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCa                                       >  1:528731/1‑140 (MQ=255)                                                                                                        aTCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAaga                               <  2:196634/140‑1 (MQ=255)                                                                                                        aTCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAaga                               <  1:625409/140‑1 (MQ=255)                                                                                                           ggTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGt                            <  1:9688/140‑1 (MQ=255)                                                                                                               agaAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGAtt                        >  2:135246/1‑140 (MQ=255)                                                                                                               agaAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGAtt                        <  2:528731/140‑1 (MQ=255)                                                                                                               agaAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGAtt                        >  2:587909/1‑140 (MQ=255)                                                                                                               agaAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGAt                         >  1:578009/1‑139 (MQ=255)                                                                                                               agaAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGAt                         <  2:578009/139‑1 (MQ=255)                                                                                                                   aCGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAAcc                    <  2:312484/140‑1 (MQ=255)                                                                                                                    cGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCa                   <  2:912375/140‑1 (MQ=255)                                                                                                                     ggATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCaa                  <  1:1145585/140‑1 (MQ=255)                                                                                                                        tgaatgaCACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAcgc               <  2:70966/140‑1 (MQ=255)                                                                                                                         gaatgaCACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAcgcg              >  2:183095/1‑140 (MQ=255)                                                                                                                             gaCACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTaa          <  1:1106020/140‑1 (MQ=255)                                                                                                                              acacTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAAc         <  1:617045/140‑1 (MQ=255)                                                                                                                              acacTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAAc         <  2:1366759/140‑1 (MQ=255)                                                                                                                               cacTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAAcc        >  1:404133/1‑140 (MQ=255)                                                                                                                                acTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAACCa       <  2:468786/140‑1 (MQ=255)                                                                                                                                acTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAACCa       <  1:297309/140‑1 (MQ=255)                                                                                                                                 cTCCGCCAGAAGAATGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAAcc                    >  1:439798/1‑126 (MQ=255)                                                                                                                                 cTCCGCCAGAAGAATGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAAcc                    <  2:439798/126‑1 (MQ=255)                                                                                                                                 cTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAACCAg      >  1:448702/1‑140 (MQ=255)                                                                                                                                    cGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTg   >  2:719344/1‑140 (MQ=255)                                                                                                                                     gCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTgg  >  2:598495/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TGAAGGTGAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGG  >  NC_000913/4246864‑4247135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑