Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 621,137 C→T A18T (GCC→ACC)  fepG ← ferric enterobactin ABC transporter membrane subunit FepG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913621,1370CT86.2% 194.2 / 21.6 80A18T (GCC→ACC) fepGferric enterobactin ABC transporter membrane subunit FepG
Reads supporting (aligned to +/- strand):  ref base C (7/4);  new base T (37/32);  total (44/36)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTC  >  NC_000913/621002‑621271
                                                                                                                                       |                                                                                                                                      
cgcgTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGaa                                                                                                                                    >  2:221094/1‑140 (MQ=255)
      gTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACcagc                                                                                                                              >  1:547567/1‑140 (MQ=255)
        aaaCGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACcagcag                                                                                                                            >  1:1307665/1‑140 (MQ=255)
        aaaCGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACcagcag                                                                                                                            <  2:264310/140‑1 (MQ=255)
        aaaCGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACcagcag                                                                                                                            >  1:319273/1‑140 (MQ=255)
        aaaCGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACcagcag                                                                                                                            >  1:1346541/1‑140 (MQ=255)
           cGCCGTTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACAAAGGCGGAAACCAGCAGCaa                                                                                                                         >  2:222238/1‑140 (MQ=255)
             ccATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAAc                                                                                                                       >  2:842750/1‑140 (MQ=255)
               aTTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAg                                                                                                                     <  1:1241788/140‑1 (MQ=255)
               aTTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAg                                                                                                                     <  1:743581/140‑1 (MQ=255)
                ttCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAgg                                                                                                                    >  1:804708/1‑140 (MQ=255)
                     tGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGtgatg                                                                                                               >  1:343375/1‑140 (MQ=255)
                       accaccATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAg                                                                                                             <  1:1119432/140‑1 (MQ=255)
                                 tCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCga                                                                                                   <  2:1238993/140‑1 (MQ=255)
                                        gcgcGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGt                                                                                            >  2:1452415/1‑140 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTaa                                                                                                          >  2:925411/1‑125 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTaa                                                                                                          <  1:925411/125‑1 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCga                                                                                                   <  1:1136851/132‑1 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCga                                                                                                   >  2:1136851/1‑132 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTa                                                                                           >  1:960279/1‑140 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTa                                                                                           >  2:586013/1‑140 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTa                                                                                           <  2:675054/140‑1 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTa                                                                                           >  2:776322/1‑140 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTa                                                                                           >  1:675054/1‑140 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTa                                                                                           >  1:486923/1‑140 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTa                                                                                           >  2:1363170/1‑140 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTa                                                                                           >  1:1049091/1‑140 (MQ=255)
                                         cgcgGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTa                                                                                           >  1:854078/1‑140 (MQ=255)
                                          gcggcgCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTaa                                                                                          >  1:996330/1‑140 (MQ=255)
                                           cggcgCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTaaa                                                                                         <  2:61866/140‑1 (MQ=255)
                                              cgcATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCa                                                                                      >  1:1246239/1‑140 (MQ=255)
                                              cgcATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCa                                                                                      >  1:943419/1‑140 (MQ=255)
                                                cATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATg                                                                                    <  2:804708/140‑1 (MQ=255)
                                                  tCGCCCATCAGCGCGGCGAATACCTGCGAGGTTCCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGca                                                                                  <  2:977948/140‑1 (MQ=255)
                                                       cATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCaccacc                                                                             <  1:542753/140‑1 (MQ=255)
                                                        aTCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCt                                                                            <  2:996330/140‑1 (MQ=255)
                                                         tCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTc                                                                           <  2:319273/140‑1 (MQ=255)
                                                         tCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTc                                                                           <  2:1307665/140‑1 (MQ=255)
                                                         tCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTc                                                                           <  1:133589/140‑1 (MQ=255)
                                                             cgcgGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAAt                                                                                        >  1:446601/1‑123 (MQ=255)
                                                             cgcgGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAAt                                                                                        <  2:446601/123‑1 (MQ=255)
                                                             cgcgGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGt                                                                       >  2:480901/1‑140 (MQ=255)
                                                               cggcgAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGttt                                                                     >  2:458870/1‑140 (MQ=255)
                                                                  cgAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTAc                                                                  >  1:841613/1‑140 (MQ=255)
                                                                   gAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACg                                                                 <  1:1452415/140‑1 (MQ=255)
                                                                   gAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACg                                                                 <  1:1273202/140‑1 (MQ=255)
                                                                      tACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCACGCGTTTTACGTCg                                                              <  2:1346541/140‑1 (MQ=255)
                                                                        ccTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCa                                                            <  1:221094/140‑1 (MQ=255)
                                                                            cGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAg                                                        >  1:1404223/1‑140 (MQ=255)
                                                                             gAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAgg                                                       <  2:341045/140‑1 (MQ=255)
                                                                                      aGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGc                                              <  1:569217/140‑1 (MQ=255)
                                                                                        cGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCAc                                            <  2:334595/140‑1 (MQ=255)
                                                                                          tGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCg                                          >  1:1310630/1‑140 (MQ=255)
                                                                                           gACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCgg                                         >  2:680860/1‑140 (MQ=255)
                                                                                           gACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACG‑GGAAGATCAGCACCGGt                                        >  1:408526/1‑140 (MQ=255)
                                                                                           gACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCgg                                         >  2:774806/1‑140 (MQ=255)
                                                                                             cGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTg                                       <  2:486923/140‑1 (MQ=255)
                                                                                             cGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTg                                       <  1:56095/140‑1 (MQ=255)
                                                                                             cGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTg                                       <  1:480901/140‑1 (MQ=255)
                                                                                              ggCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGc                                      <  1:1363170/140‑1 (MQ=255)
                                                                                                  ccGCTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCa                                  >  1:311968/1‑140 (MQ=255)
                                                                                                  ccGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCa                                  >  1:126127/1‑140 (MQ=255)
                                                                                                     cTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTg                                       <  2:208161/132‑1 (MQ=255)
                                                                                                     cTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTg                                       >  1:208161/1‑132 (MQ=255)
                                                                                                     cTGCGTAATCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATa                               >  2:1078763/1‑140 (MQ=255)
                                                                                                           aaTCCCCAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCa                         <  2:960279/140‑1 (MQ=255)
                                                                                                           aaTCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCTCCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCa                         <  2:1049091/140‑1 (MQ=255)
                                                                                                               cccAGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGa                     >  2:547477/1‑140 (MQ=255)
                                                                                                                  aGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCa                         >  1:344591/1‑133 (MQ=255)
                                                                                                                  aGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCa                         <  2:344591/133‑1 (MQ=255)
                                                                                                                  aGATACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACca                  <  2:943419/140‑1 (MQ=255)
                                                                                                                    aTACCTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACcaca                <  2:1246239/140‑1 (MQ=255)
                                                                                                                       ccTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGaa             >  1:249859/1‑140 (MQ=255)
                                                                                                                       ccTGCAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGaa             >  1:1007149/1‑140 (MQ=255)
                                                                                                                           cAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAAcgc         >  2:846699/1‑140 (MQ=255)
                                                                                                                           cAACCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAAcgc         <  1:458870/140‑1 (MQ=255)
                                                                                                                            aaCCACACAGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAAcgcg        <  2:1131880/140‑1 (MQ=255)
                                                                                                                              ccACACAGGTGGAAACCAGCAGCGAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCa      >  2:668061/1‑140 (MQ=255)
                                                                                                                                 cacaGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGtt   >  1:1369421/1‑140 (MQ=255)
                                                                                                                                  acaGGTGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTc  >  2:1026082/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATCATGCACCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTC  >  NC_000913/621002‑621271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑