Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,265,777	C→T	intergenic (+48/‑5)	dnaB → / → alr	replicative DNA helicase/alanine racemase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,265,777	0	C	T	100.0%	226.7 / NA	66	intergenic (+48/‑5)	dnaB/alr	replicative DNA helicase/alanine racemase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/32);  total (34/32)

AACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAG  >  NC_000913/4265640‑4265913                                                                                                                                          |                                                                                                                                         aaCGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAatac                                                                                                                                        >  1:126529/1‑140 (MQ=255)   cGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACaa                                                                                                                                      >  1:1342526/1‑140 (MQ=255)   cGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACaa                                                                                                                                      >  2:70764/1‑140 (MQ=255)     gCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAAt                                                                                                                                    >  2:877557/1‑140 (MQ=255)      cccAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATg                                                                                                                                   >  2:1381433/1‑140 (MQ=255)         aaTCGGGACGGTTCGCCTGACCTTTAACGGTCAATGGTCGCGTTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCaa                                                                                                                                <  2:210208/140‑1 (MQ=255)               gACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCa                                                                                                                          <  1:40862/140‑1 (MQ=255)               gACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCa                                                                                                                          <  1:879111/140‑1 (MQ=255)                aCGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATATTAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCaa                                                                                                                         <  1:1266597/140‑1 (MQ=255)                aCGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCaa                                                                                                                         <  1:1033920/140‑1 (MQ=255)                aCGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCaa                                                                                                                         <  2:777263/140‑1 (MQ=255)                aCGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCaa                                                                                                                         <  2:1342526/140‑1 (MQ=255)                    tACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAACAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACtgt                                                                                                                     >  1:1145036/1‑140 (MQ=255)                            aCCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTa                                                                                                             >  2:741114/1‑140 (MQ=255)                            aCCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTa                                                                                                             >  1:479114/1‑140 (MQ=255)                             ccTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTaa                                                                                                            <  1:979281/140‑1 (MQ=255)                              cTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGATTTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAAc                                                                                                           >  2:1058884/1‑140 (MQ=255)                                ttAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAAccg                                                                                                         <  1:338810/140‑1 (MQ=255)                                 tAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAAccgc                                                                                                        >  1:417707/1‑140 (MQ=255)                                  aaCGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAAccgcc                                                                                                       >  2:92452/1‑140 (MQ=255)                                       tCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGct                                                                                                  >  1:482168/1‑140 (MQ=255)                                       tCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGct                                                                                                  >  1:53326/1‑140 (MQ=255)                                        cAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGctc                                                                                                 >  1:377581/1‑140 (MQ=255)                                               cgcgCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGaca                                                                                          <  2:479114/140‑1 (MQ=255)                                                     tCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCt                                                                                    >  1:498636/1‑140 (MQ=255)                                                      cGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTg                                                                                   <  1:1325941/140‑1 (MQ=255)                                                      cGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTg                                                                                   <  1:877557/140‑1 (MQ=255)                                                           aCTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACg                                                                              <  2:878510/140‑1 (MQ=255)                                                           aCTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACg                                                                              <  2:1193391/140‑1 (MQ=255)                                                           aCTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCCGCGACACAACCTGCAACg                                                                              <  2:417707/140‑1 (MQ=255)                                                            cTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGt                                                                             >  2:1315856/1‑140 (MQ=255)                                                                gCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTc                                                                         <  1:151761/140‑1 (MQ=255)                                                                gCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTc                                                                         <  1:1417458/140‑1 (MQ=255)                                                                     gCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGaa                                                                    <  2:377581/140‑1 (MQ=255)                                                                      ccGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAAc                                                                   >  1:238701/1‑140 (MQ=255)                                                                      ccGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAAc                                                                   >  1:256385/1‑140 (MQ=255)                                                                      ccGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAAc                                                                   >  1:1156323/1‑140 (MQ=255)                                                                       cGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACt                                                                  >  2:783648/1‑140 (MQ=255)                                                                         cAGTACGACGACGAATAAGAATTATTTTATGAATTAGGAAATTAAATCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTgg                                                                >  2:1412094/1‑140 (MQ=255)                                                                          aGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGc                                                               <  1:646040/140‑1 (MQ=255)                                                                           gTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGcc                                                              <  2:716325/140‑1 (MQ=255)                                                                              cgacgacgaATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGTCCCCt                                                           >  1:756381/1‑140 (MQ=255)                                                                              cgacgacgaATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGACAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCt                                                           <  1:48441/140‑1 (MQ=255)                                                                              cgaagacgaAAAATAATTATTTTATGAATTAGGTAATTAAAGCAACCGCTTACCAAGAAATAAAAAGGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCt                                                           <  2:1145036/140‑1 (MQ=255)                                                                                   acgaATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAg                                                      >  2:490929/1‑140 (MQ=255)                                                                                   acgaATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAg                                                      <  1:741114/140‑1 (MQ=255)                                                                                    cgaATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGt                                                     >  1:410001/1‑140 (MQ=255)                                                                                      aataataatTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTaa                                                   >  1:703317/1‑140 (MQ=255)                                                                                                attTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                                         <  1:402418/140‑1 (MQ=255)                                                                                                attTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                                         <  1:490929/140‑1 (MQ=255)                                                                                                attTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                                         >  2:402418/1‑140 (MQ=255)                                                                                                 ttttATGAATTAGGAAATTAAAGCAAACACTTATCAAGGAATAAAAAGGCAAGGGGCAACTTTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCgg                                        <  2:482168/140‑1 (MQ=255)                                                                                                   ttATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCggtg                                      >  2:1146074/1‑140 (MQ=255)                                                                                                           tAGGTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGc                              >  1:896652/1‑140 (MQ=255)                                                                                                            aGGTAATAAAAGCAAACGCTTATAAAGGAATACAAATGCAAGCGGAACCTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCg                             <  1:916650/140‑1 (MQ=255)                                                                                                             ggTAATTAAAGAAAACACTTATCAAGGAATACAAATGCAAGCGGCACCTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGa                            <  1:689927/140‑1 (MQ=255)                                                                                                              gTAATTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGaa                           <  1:92452/140‑1 (MQ=255)                                                                                                                aaTTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACg                         <  1:70764/140‑1 (MQ=255)                                                                                                                aaTTAAAGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACg                         >  1:823632/1‑140 (MQ=255)                                                                                                                    aaaGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTa                     <  2:498636/140‑1 (MQ=255)                                                                                                                    aaaGCAAACACTTATCAAGGAATAAAAATGCAAGCGGAAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTa                     <  2:126529/140‑1 (MQ=255)                                                                                                                      aGCAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATg                   >  2:1238463/1‑140 (MQ=255)                                                                                                                        cAAACACTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGt                 >  1:1448531/1‑140 (MQ=255)                                                                                                                            cccTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACg             <  1:1381433/138‑1 (MQ=255)                                                                                                                            cacTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACg             <  2:1156323/140‑1 (MQ=255)                                                                                                                            cacTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACg             <  1:783648/140‑1 (MQ=255)                                                                                                                              cTTATCAGGGAAAACAAATCCAAGCGGCAACTGTTGTGATAAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGt           <  2:896652/140‑1 (MQ=255)                                                                                                                              cTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGt           <  2:823632/140‑1 (MQ=255)                                                                                                                              cTTATCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGt           <  2:256385/140‑1 (MQ=255)                                                                                                                              cTNATCAAGAAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGt           <  2:912390/140‑1 (MQ=255)                                                                                                                                 aTCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGtctt        >  2:1361620/1‑140 (MQ=255)                                                                                                                                 aTCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGtctt        >  1:335877/1‑140 (MQ=255)                                                                                                                                 aTCAAGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGtctt        >  1:928661/1‑140 (MQ=255)                                                                                                                                     aGGAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTg    >  2:1085441/1‑140 (MQ=255)                                                                                                                                       gAATACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTgag  >  1:329731/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         AACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAG  >  NC_000913/4265640‑4265913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑