Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,522,428	C→T	G556D (GGC→GAC)	yjhG ←	KpLE2 phage‑like element; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,522,428	0	C	T	100.0%	292.3 / NA	83	G556D (GGC→GAC)	yjhG	KpLE2 phage‑like element; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/35);  total (48/35)

ATTGCAGTTGCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTC  >  NC_000913/4522296‑4522564                                                                                                                                     |                                                                                                                                         atTGCAGTTGCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGc                                                                                                                                   <  1:989723/140‑1 (MQ=255)   tGCAGTTGCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCa                                                                                                                                 >  2:1005352/1‑140 (MQ=255)     cAGTTGCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAgc                                                                                                                               <  2:1098873/140‑1 (MQ=255)     cAGTTGCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAgc                                                                                                                               >  1:1445215/1‑140 (MQ=255)     cAGTTGCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAgc                                                                                                                               >  1:875645/1‑140 (MQ=255)        ttGCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCt                                                                                                                            >  2:1116114/1‑140 (MQ=255)          gCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTc                                                                                                                          >  2:30440/1‑140 (MQ=255)          gCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTc                                                                                                                          >  1:243350/1‑140 (MQ=255)             tcctcctGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTgg                                                                                                                       <  2:378443/140‑1 (MQ=255)                  ctGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCca                                                                                                                  <  2:880921/140‑1 (MQ=255)                    gtgAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCcaca                                                                                                                >  1:124814/1‑140 (MQ=255)                    gtgAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCcaca                                                                                                                <  1:761421/140‑1 (MQ=255)                     tgAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACAt                                                                                                               >  2:385615/1‑140 (MQ=255)                     tgAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACAt                                                                                                               >  1:1202718/1‑140 (MQ=255)                       aaGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATgg                                                                                                             >  2:875452/1‑140 (MQ=255)                        aGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGc                                                                                                            <  1:505858/140‑1 (MQ=255)                         ggTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGcc                                                                                                           >  2:472801/1‑140 (MQ=255)                         ggTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGcc                                                                                                           >  2:1340746/1‑140 (MQ=255)                          gTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCg                                                                                                          >  1:218835/1‑140 (MQ=255)                               tGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCa                                                                                                     >  1:883132/1‑140 (MQ=255)                               tGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCa                                                                                                     <  2:124814/140‑1 (MQ=255)                                 ttCAACGGTTCGGGTTCCGGGGAAATTGGCTTCGCCCTGAAGCTCCCGACAATCCATTTTAAATTCAATTAAATCCCCGGGGCGTAATTTTCCGATGGGGTTCCCCGCCAGCGCTTCTGGCCCCACATGGCCGATGCAcg                                                                                                   <  2:734713/140‑1 (MQ=255)                                   cATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCAcgcg                                                                                                 <  2:883132/140‑1 (MQ=255)                                     tCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGcc                                                                                               <  1:1340746/140‑1 (MQ=255)                                      cGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCa                                                                                              <  2:218835/140‑1 (MQ=255)                                      cGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCa                                                                                              >  2:1202357/1‑140 (MQ=255)                                         tACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGcc                                                                                               <  1:1322476/136‑1 (MQ=255)                                         tACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGcc                                                                                               >  2:1322476/1‑136 (MQ=255)                                         tACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTa                                                                                           >  2:1198283/1‑140 (MQ=255)                                           cGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGa                                                                                         >  1:1050954/1‑140 (MQ=255)                                           cGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGa                                                                                         >  1:862610/1‑140 (MQ=255)                                            gggTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGaa                                                                                        >  1:875465/1‑140 (MQ=255)                                             ggTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGaaa                                                                                       <  1:1090070/140‑1 (MQ=255)                                             ggTCCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTGAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCCCATGGCCGATGCACGCGCCAGTAGaag                                                                                       <  1:1091487/140‑2 (MQ=255)                                                tCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGaa                                                                                        <  1:151241/136‑1 (MQ=255)                                                tCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGaa                                                                                        >  2:151241/1‑136 (MQ=255)                                                tCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAAc                                                                                      <  1:1357322/138‑1 (MQ=255)                                                tCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAAc                                                                                      >  2:1357322/1‑138 (MQ=255)                                                  cGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGccc                                                                                  <  1:30440/140‑1 (MQ=255)                                                   gAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCg                                                                                 >  2:341345/1‑140 (MQ=255)                                                   gAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCg                                                                                 >  1:1140303/1‑140 (MQ=255)                                                   gAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCAGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCg                                                                                 >  1:1319577/1‑140 (MQ=255)                                                    aGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGAGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCga                                                                                <  2:284258/140‑1 (MQ=255)                                                        aaTTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGaaa                                                                            <  1:1116114/140‑1 (MQ=255)                                                        aaTTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGaaa                                                                            >  2:1235238/1‑140 (MQ=255)                                                         aTTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAAc                                                                           >  1:1060881/1‑140 (MQ=255)                                                              cTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCa                                                                      <  2:875645/140‑1 (MQ=255)                                                                 cGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCg                                                                   <  1:875452/140‑1 (MQ=255)                                                                      tGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGAt                                                              >  1:982836/1‑140 (MQ=255)                                                                        aaGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTa                                                            <  1:1235238/140‑1 (MQ=255)                                                                          gCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAAc                                                          <  1:79518/140‑1 (MQ=255)                                                                            tCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGa                                                        <  2:982836/140‑1 (MQ=255)                                                                                    atcaatTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCt                                                <  1:1391539/140‑1 (MQ=255)                                                                                     tcaatTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCtt                                               >  1:860205/1‑140 (MQ=255)                                                                                      caatTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTa                                              <  1:1384180/140‑1 (MQ=255)                                                                                      caatTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTa                                              <  2:243350/140‑1 (MQ=255)                                                                                      caatTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCAGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTa                                              <  2:1319577/140‑1 (MQ=255)                                                                                          tttAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGt                                          >  2:1433357/1‑140 (MQ=255)                                                                                          tttAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTCCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGt                                          >  2:314881/1‑140 (MQ=255)                                                                                            tAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTAt                                        >  2:105865/1‑140 (MQ=255)                                                                                               tttCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGAc                                     >  1:455845/1‑140 (MQ=255)                                                                                                         aTCCCCGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCa                           <  2:862610/140‑1 (MQ=255)                                                                                                            cccGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAggg                        <  2:1202718/140‑1 (MQ=255)                                                                                                            cccGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAggg                        <  2:455845/140‑1 (MQ=255)                                                                                                             ccGGTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGc                       >  2:905095/1‑140 (MQ=255)                                                                                                               ggTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCAc                     >  2:1347577/1‑140 (MQ=255)                                                                                                               ggTGCGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCAc                     >  2:12592/1‑140 (MQ=255)                                                                                                                   cGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGt                 >  2:854013/1‑140 (MQ=255)                                                                                                                   cGTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGt                 >  2:835129/1‑140 (MQ=255)                                                                                                                    gTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTa                <  1:240770/140‑1 (MQ=255)                                                                                                                    gTAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTa                >  1:859437/1‑140 (MQ=255)                                                                                                                     tAATTTACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGAGCACTGGTaa               >  2:535144/1‑140 (MQ=255)                                                                                                                          tACCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTgg          <  1:854013/140‑1 (MQ=255)                                                                                                                           aCCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTgg          <  1:396602/139‑1 (MQ=255)                                                                                                                           aCCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTgg          >  2:396602/1‑139 (MQ=255)                                                                                                                           aCCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGt         >  2:739472/1‑140 (MQ=255)                                                                                                                           aCCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGt         >  2:778743/1‑140 (MQ=255)                                                                                                                           aCCGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGt         >  1:192357/1‑140 (MQ=255)                                                                                                                             cGATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAg       <  2:1060881/140‑1 (MQ=255)                                                                                                                              gATGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAgg      <  1:24432/140‑1 (MQ=255)                                                                                                                               aTGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGt     >  2:212058/1‑140 (MQ=255)                                                                                                                               aTGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGt     >  2:1112593/1‑140 (MQ=255)                                                                                                                               aTGGGGTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGt     >  1:617965/1‑140 (MQ=255)                                                                                                                                gggggACTCCAGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGtt    >  2:1003029/2‑140 (MQ=255)                                                                                                                                  gggTCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTttc  >  1:1333684/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                         ATTGCAGTTGCCTCCTCCTGTGAAGGTAATTGTTCATCGCTACGGGTTCCGAGGAAATTGACTTCGCCGTGAAGCTCGCGACAATCAATTTTAATTTCAATTAAATCCCCGGTGCGTAATTTACCGATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTC  >  NC_000913/4522296‑4522564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑