Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,560,658	A→G	V8A (GTA→GCA)	yjiH ←	Gate family protein YjiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,560,658	0	A	G	100.0%	322.9 / NA	87	V8A (GTA→GCA)	yjiH	Gate family protein YjiH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/45);  total (42/45)

TCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTG  >  NC_000913/4560522‑4560792                                                                                                                                          |                                                                                                                                       tCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAg                                                                                                                                     <  1:680304/140‑1 (MQ=255) tCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAg                                                                                                                                     >  2:468838/1‑140 (MQ=255)     ttCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATc                                                                                                                                 >  2:764946/1‑140 (MQ=255)      tCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATcc                                                                                                                                <  2:375974/140‑1 (MQ=255)       cAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTgcagca                                                                                                                                   >  1:785530/1‑136 (MQ=255)       cAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTgcagca                                                                                                                                   <  2:785530/136‑1 (MQ=255)          aaCGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCtt                                                                                                                            >  2:536066/1‑140 (MQ=255)           aCGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttg                                                                                                                           >  2:392876/1‑140 (MQ=255)           aCGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttg                                                                                                                           <  1:685838/140‑1 (MQ=255)            cGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttgt                                                                                                                          <  1:1239720/140‑1 (MQ=255)            cGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttgt                                                                                                                          <  2:1057389/140‑1 (MQ=255)            cGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttgt                                                                                                                          >  1:544380/1‑140 (MQ=255)            cGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttgt                                                                                                                          <  2:109424/140‑1 (MQ=255)             ggAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttgt                                                                                                                          <  2:370786/140‑1 (MQ=255)             ggAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttgtt                                                                                                                         >  1:787215/1‑140 (MQ=255)             ggAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttgtt                                                                                                                         <  1:468838/140‑1 (MQ=255)              gAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttgttg                                                                                                                        <  2:132113/140‑1 (MQ=255)                 aaaTCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTgtgt                                                                                                                     <  1:542403/140‑1 (MQ=255)                 aaaTCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTgtgt                                                                                                                     <  2:787215/140‑1 (MQ=255)                 aaaTCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTgtgt                                                                                                                     <  1:699722/140‑1 (MQ=255)                 aaaTCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTgtgt                                                                                                                     <  1:1075228/140‑1 (MQ=255)                   aTCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCa                                                                                                                   <  1:101222/140‑1 (MQ=255)                    tCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttg                                                                                                                           >  2:1394116/1‑131 (MQ=255)                    tCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCttg                                                                                                                           <  1:1394116/131‑1 (MQ=255)                    tCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCAt                                                                                                                  >  2:850838/1‑140 (MQ=255)                     cAAAAACGCGCCACCAGCTGTCTGTTCCGGAATAAACGCCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATa                                                                                                                 <  1:868928/140‑1 (MQ=255)                          aCGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAAt                                                                                                            <  1:1092643/140‑1 (MQ=255)                               ccaccaGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCa                                                                                                       <  1:850838/140‑1 (MQ=255)                               ccaccaGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCa                                                                                                       <  2:953697/140‑1 (MQ=255)                               ccaccaGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCa                                                                                                       <  2:216026/140‑1 (MQ=255)                                        gtctgtTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCa                                                                                                       <  1:1450284/131‑1 (MQ=255)                                        gtctgtTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCa                                                                                                       >  2:1450284/1‑131 (MQ=255)                                          ctgtTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttg                                                                                            <  1:91269/140‑1 (MQ=255)                                           tgtTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgt                                                                                           >  1:1225983/1‑140 (MQ=255)                                                cGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgtgtttg                                                                                      >  1:537784/1‑140 (MQ=255)                                                cGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgtgtttg                                                                                      >  1:156859/1‑140 (MQ=255)                                                cGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgtgtttg                                                                                      >  2:851134/1‑140 (MQ=255)                                                   agaaaaCACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTttgtgtttgtgt                                                                                   >  2:953482/3‑140 (MQ=255)                                                   aaaaaaCACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTttgtgtttgtgt                                                                                   >  2:1297733/1‑140 (MQ=255)                                                       aaCACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTg                                                                               >  2:508466/1‑140 (MQ=255)                                                         cacCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCt                                                                             <  1:536066/140‑1 (MQ=255)                                                             gg‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAg                                                                         >  1:640313/1‑140 (MQ=255)                                                                    aaaGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGa                                                                   >  1:710184/1‑140 (MQ=255)                                                                         ttATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAAtt                                                              >  1:49863/1‑140 (MQ=255)                                                                          tATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTc                                                             >  2:99590/1‑140 (MQ=255)                                                                               tGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGa                                                        <  2:640313/140‑1 (MQ=255)                                                                                gagaAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAg                                                                     >  1:828192/1‑126 (MQ=255)                                                                                gagaAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAg                                                                     <  2:828192/126‑1 (MQ=255)                                                                                 agaAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAAc                                                      >  2:854001/1‑140 (MQ=255)                                                                                 agaAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAAc                                                      >  2:724584/1‑140 (MQ=255)                                                                                    aaaaaGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGa                                                   <  1:1041134/140‑1 (MQ=255)                                                                                       aaGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGaaaa                                                <  1:851134/140‑1 (MQ=255)                                                                                       aaGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGaaaa                                                >  1:1168520/1‑140 (MQ=255)                                                                                       aaGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGaaaa                                                >  2:451610/1‑140 (MQ=255)                                                                                         ggCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAAc                                              >  2:440010/1‑140 (MQ=255)                                                                                         ggCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAAc                                              <  2:49863/140‑1 (MQ=255)                                                                                             aGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTa                                          >  2:1122663/1‑140 (MQ=255)                                                                                             aGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTa                                          >  2:342845/1‑140 (MQ=255)                                                                                             aGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTa                                          <  2:544380/140‑1 (MQ=255)                                                                                              gATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTAt                                         <  2:1206510/140‑1 (MQ=255)                                                                                              gATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTAt                                         <  1:392876/140‑1 (MQ=255)                                                                                                 aCCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAg                                      <  1:690179/140‑1 (MQ=255)                                                                                                  cccTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCTGGAAAATTCCTGGAACTGAAAAACCTTagcagc                                     <  2:149397/140‑1 (MQ=255)                                                                                                  cccTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGc                                     <  1:440010/140‑1 (MQ=255)                                                                                                     tttAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCAt                                  <  1:1297733/140‑1 (MQ=255)                                                                                                     tttAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCAt                                  >  1:1393787/1‑140 (MQ=255)                                                                                                      ttAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCAtg                                 <  1:961829/140‑1 (MQ=255)                                                                                                      ttAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCAtg                                 <  2:1168520/140‑1 (MQ=255)                                                                                                      ttAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCAtg                                 <  2:537784/140‑1 (MQ=255)                                                                                                      ttAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCAtg                                 <  1:451610/140‑1 (MQ=255)                                                                                                            cccACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTa                           >  1:25245/1‑140 (MQ=255)                                                                                                             ccACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCAtgt                                >  1:554708/1‑134 (MQ=255)                                                                                                             ccACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCAtgt                                <  2:554708/134‑1 (MQ=255)                                                                                                             ccACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTAt                          >  1:739820/1‑140 (MQ=255)                                                                                                             ccACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTAt                          >  1:573645/1‑140 (MQ=255)                                                                                                             ccACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTAt                          <  2:739820/140‑1 (MQ=255)                                                                                                             ccACTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGCAt                          >  2:1248366/1‑140 (MQ=255)                                                                                                               aCTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATgg                        >  2:1406872/1‑140 (MQ=255)                                                                                                               aCTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATgg                        >  1:1417651/1‑140 (MQ=255)                                                                                                                cTTTTTCTGTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATggg                       >  2:544601/1‑140 (MQ=255)                                                                                                                        gTCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGc               <  1:764946/140‑1 (MQ=255)                                                                                                                         tCGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCa              >  2:1113540/1‑140 (MQ=255)                                                                                                                          cGCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCaa             <  1:724584/140‑1 (MQ=255)                                                                                                                           gCCAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAg            >  1:367423/1‑140 (MQ=255)                                                                                                                            ccAGCTCCCCGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGt           <  1:1113540/140‑1 (MQ=255)                                                                                                                                   cccGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTc    >  2:614738/1‑140 (MQ=255)                                                                                                                                     cGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAAt                 <  1:566305/125‑1 (MQ=255)                                                                                                                                     cGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAAt                 >  2:566305/1‑125 (MQ=255)                                                                                                                                     cGGCTGCAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTg  >  1:653404/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGG‑AAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTG  >  NC_000913/4560522‑4560792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑