Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	761,033	G→A	M776I (ATG→ATA)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	761,033	0	G	A	100.0%	335.8 / NA	94	M776I (ATG→ATA)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/45);  total (49/45)

CGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTAGTGATGTGT  >  NC_000913/760898‑761165                                                                                                                                        |                                                                                                                                     cgcgcgTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGc                                                                                                                                  <  1:1212009/140‑1 (MQ=255)  gcgcgTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGcc                                                                                                                                 <  2:1046540/140‑1 (MQ=255)  gcgcgTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGcc                                                                                                                                 <  1:1342177/140‑1 (MQ=255)  gcgcgTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGcc                                                                                                                                 <  1:1339034/140‑1 (MQ=255)    gcgTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGa                                                                                                                               <  2:1336898/140‑1 (MQ=255)    gcgTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGa                                                                                                                               <  2:1399096/140‑1 (MQ=255)      gTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGATCGTGATATCGCCGaaa                                                                                                                             >  1:1147243/1‑140 (MQ=255)       tCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCATGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAAt                                                                                                                            <  2:31195/140‑1 (MQ=255)       tCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAAt                                                                                                                            <  1:1124163/140‑1 (MQ=255)       tCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAAt                                                                                                                            <  2:504935/140‑1 (MQ=255)              cGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCCGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCctgct                                                                                                                     <  2:549173/140‑1 (MQ=255)                ttATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCtgctgc                                                                                                                   >  2:263501/1‑140 (MQ=255)                  aTCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGt                                                                                                                 >  2:185944/1‑140 (MQ=255)                    cTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCa                                                                                                               >  2:296840/1‑140 (MQ=255)                     tGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCAt                                                                                                              >  1:276072/1‑140 (MQ=255)                            ttGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGATCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTgg                                                                                                       <  2:1147243/140‑1 (MQ=255)                             tgtgCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTgg                                                                                                       >  2:824100/1‑139 (MQ=255)                             tgtgCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTgg                                                                                                       <  1:824100/139‑1 (MQ=255)                             tgtgCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGc                                                                                                      >  2:896118/1‑140 (MQ=255)                             tgtgCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGc                                                                                                      >  1:178094/1‑140 (MQ=255)                             tgtgCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGc                                                                                                      <  1:1423376/140‑1 (MQ=255)                               tgCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCgg                                                                                                    >  2:1409436/1‑140 (MQ=255)                               tgCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCgg                                                                                                    >  2:344638/1‑140 (MQ=255)                                         aaCATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCt                                                                                          >  1:1447239/1‑140 (MQ=255)                                           cATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCg                                                                                        <  2:1447239/140‑1 (MQ=255)                                              gCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCgaag                                                                                     >  2:1365343/1‑140 (MQ=255)                                                aGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCgaagaa                                                                                   <  1:306227/140‑1 (MQ=255)                                                   tttGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTg                                                                                >  1:1390557/1‑140 (MQ=255)                                                   tttGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTg                                                                                >  2:232350/1‑140 (MQ=255)                                                     tGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGc                                                                              >  1:929651/1‑140 (MQ=255)                                                      gCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCg                                                                             <  1:1365343/140‑1 (MQ=255)                                                       cGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGa                                                                            >  1:1276397/1‑140 (MQ=255)                                                           ccGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACgg                                                                        <  1:263501/140‑1 (MQ=255)                                                                tACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCt                                                                   >  2:529439/1‑140 (MQ=255)                                                                        cacaGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgcca                                                           >  1:479570/1‑140 (MQ=255)                                                                        cacaGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgcca                                                           >  1:457100/1‑140 (MQ=255)                                                                        cacaGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgcca                                                           >  2:534588/1‑140 (MQ=255)                                                                        cacaGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgcca                                                           >  2:32186/1‑140 (MQ=255)                                                                        cacaGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgcc                                                            <  2:517306/139‑1 (MQ=255)                                                                        cacaGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgcc                                                            >  1:517306/1‑139 (MQ=255)                                                                            ggTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgccagcca                                                       >  1:529740/1‑140 (MQ=255)                                                                             gTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgccagcca                                                       >  1:1294251/1‑139 (MQ=255)                                                                             gTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgccagcca                                                       >  2:221488/1‑139 (MQ=255)                                                                             gTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgccagcca                                                       <  1:221488/139‑1 (MQ=255)                                                                             gTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTgccagcca                                                       <  2:1294251/139‑1 (MQ=255)                                                                             gTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCAt                                                      >  2:776563/1‑140 (MQ=255)                                                                             gTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCAt                                                      >  1:1037909/1‑140 (MQ=255)                                                                             gTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCAt                                                      >  1:152897/1‑140 (MQ=255)                                                                             gTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCAt                                                      >  1:691424/1‑140 (MQ=255)                                                                                tACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCgg                                                   <  2:1037909/140‑1 (MQ=255)                                                                                      aTGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAAt                                             >  1:600250/1‑140 (MQ=255)                                                                                       tgctgcGTCGTCAGGCGCTGTGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATc                                            >  2:796711/1‑140 (MQ=255)                                                                                       tgctgcGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATc                                            >  2:316774/1‑140 (MQ=255)                                                                                       tgctgcGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATc                                            >  2:349738/1‑140 (MQ=255)                                                                                       tgctgcGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATc                                            >  2:381720/1‑140 (MQ=255)                                                                                       tgctgcGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATc                                            >  1:1130823/1‑140 (MQ=255)                                                                                       tgctgcGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATc                                            >  1:928568/1‑140 (MQ=255)                                                                                        gctgcGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATcg                                           >  1:386137/1‑140 (MQ=255)                                                                                            cgtcgtCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGTCTCGAAGAACTGGCGAACGGCACCTTCCTgccagcca                                                       <  1:1357545/124‑1 (MQ=255)                                                                                            cgtcgtCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGTCTCGAAGAACTGGCGAACGGCACCTTCCTgccagcca                                                       >  2:1357545/1‑124 (MQ=255)                                                                                            cgtcgtCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATcgacga                                       >  2:100553/1‑140 (MQ=255)                                                                                             gtcgtcAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAg                                      >  1:71458/1‑140 (MQ=255)                                                                                              tcgtcAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGc                                     <  2:928568/140‑1 (MQ=255)                                                                                              tcgtcAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGc                                     <  1:529439/140‑1 (MQ=255)                                                                                                gtcAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCtt                                   >  1:1172188/1‑140 (MQ=255)                                                                                                gtcAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCtt                                   <  1:349738/140‑1 (MQ=255)                                                                                                gtcAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCtt                                   <  1:316774/140‑1 (MQ=255)                                                                                                       gcTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGTCATCGGTGAAATCGACGAGCTTGATCCGa                            <  1:534588/140‑1 (MQ=255)                                                                                                       gcTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGa                            <  2:929651/140‑1 (MQ=255)                                                                                                       gcTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGa                            <  1:232350/140‑1 (MQ=255)                                                                                                       gcTGCGCGGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGa                            >  1:297370/1‑140 (MQ=255)                                                                                                           cgcgGGATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAggg                        >  2:133301/1‑140 (MQ=255)                                                                                                           cgcgGGATGCGTCGTCCGCTGGTCGTGATATCGACGAAATCCCTGCTGTGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAggg                        >  2:1452924/1‑140 (MQ=255)                                                                                                              gggATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGt                     <  1:32186/140‑1 (MQ=255)                                                                                                               ggATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTg                    >  2:1349918/1‑140 (MQ=255)                                                                                                               ggATGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTg                    <  2:386137/140‑1 (MQ=255)                                                                                                                 aTGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGaa                  <  2:1276397/140‑1 (MQ=255)                                                                                                                 aTGCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGaa                  <  2:529740/140‑1 (MQ=255)                                                                                                                   gCGTCGTCCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAgc                >  2:1393464/1‑140 (MQ=255)                                                                                                                     gtcgtcCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAgcgc              >  1:1013929/1‑140 (MQ=255)                                                                                                                      tcgtcCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGcgcg             <  1:1393464/140‑1 (MQ=255)                                                                                                                      tcgtcCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGcgcg             <  2:178094/140‑1 (MQ=255)                                                                                                                        gtcCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTa           <  2:457100/140‑1 (MQ=255)                                                                                                                        gtcCGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTa           <  2:297370/140‑1 (MQ=255)                                                                                                                          ccGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTAGt         >  2:913333/1‑140 (MQ=255)                                                                                                                           cGCTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTAGTg        <  1:185944/140‑1 (MQ=255)                                                                                                                             cTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTAGTGAt      <  2:600250/140‑1 (MQ=255)                                                                                                                             cTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTAGTGAt      <  2:71458/140‑1 (MQ=255)                                                                                                                             cTGGTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGATTGATCCGAAGGGCGTGAAGCGCGTAGTGAt      <  2:1072573/140‑1 (MQ=255)                                                                                                                             cTGGTCGTGATATCGACGAAATCCCTGCTGTGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTAGTGAt      <  1:1452924/140‑1 (MQ=255)                                                                                                                                gTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGcgcg             <  1:34354/130‑1 (MQ=255)                                                                                                                                gTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGcgcg             >  2:34354/1‑130 (MQ=255)                                                                                                                                gTCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTAGTGAtgtg   <  2:806453/140‑1 (MQ=255)                                                                                                                                 tCGTGATATCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTAGTGATgtgt  <  2:1013929/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     CGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCTGCCAGCCATCGGTGAAATCGACGAGCTTGATCCGAAGGGCGTGAAGCGCGTAGTGATGTGT  >  NC_000913/760898‑761165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑