Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT27.0% 65.0 / 16.6 37intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (1/26);  new base T (10/0);  total (11/26)
Fisher's exact test for biased strand distribution p-value = 3.16e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAA  >  NC_000913/3206296‑3206569
                                                                                                                                           |                                                                                                                                      
gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTc                                                                                                                                        <  1:759309/140‑1 (MQ=255)
    aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGATAATTTTTTCata                                                                                                                                    >  2:388299/1‑138 (MQ=255)
      cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACa                                                                                                                                  <  1:1112209/140‑1 (MQ=255)
         tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTGACaaaa                                                                                                                               >  2:88750/1‑139 (MQ=255)
           ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                             <  1:935596/140‑1 (MQ=255)
                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTCTACAAATACaaaaa                                                                                                                        >  2:116964/1‑140 (MQ=255)
                     ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCTAACAaaaaaaaaaaaaggg                                                                                                                   >  2:845588/1‑135 (MQ=255)
                      tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                  <  1:606158/140‑1 (MQ=255)
                        tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAAAAAAAAATGGAggt                                                                                                                >  2:54178/1‑138 (MQ=255)
                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGTTgg                                                                                                              >  2:56270/1‑139 (MQ=255)
                           cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGcc                                                                                                             <  1:152082/140‑1 (MQ=255)
                           cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATATTTCCATACACACACAAAAACTgggggtacc                                                                                                             >  2:1434178/1‑133 (MQ=255)
                              aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatg                                                                                                          <  1:845588/140‑1 (MQ=255)
                                   tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                     <  1:104984/140‑1 (MQ=255)
                                   tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                     <  2:411624/140‑1 (MQ=255)
                                     attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                   <  2:126158/140‑1 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                  <  1:421606/140‑1 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                  <  1:252634/140‑1 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                  <  2:1378626/140‑1 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                  <  2:1378327/140‑1 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                  <  1:388299/140‑1 (MQ=255)
                                            ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGTTGCCATGATGAGCGgaaagaa                                                                                            >  2:585902/1‑136 (MQ=255)
                                            ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAAAGGAGGTGCCCTTATGAGGGaaaaaaa                                                                                            >  2:1159551/1‑136 (MQ=255)
                                             tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                           <  2:1050111/140‑1 (MQ=255)
                                                    ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                    <  1:1159551/140‑1 (MQ=255)
                                                     tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                   <  1:56270/140‑1 (MQ=255)
                                                       aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAAATGAGGTTGCATGATGAGCGCAAAAAATAAACaaaaag                                                                                 >  2:1088549/1‑136 (MQ=255)
                                                       aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                 <  2:1532016/140‑1 (MQ=255)
                                                            cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAATAAAAAAAATTGAGGTGGCCTGATTAGGGAAAAAAATAAACAAAAAGCAggg                                                                            >  2:132667/1‑138 (MQ=255)
                                                            cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                            <  1:116964/140‑1 (MQ=255)
                                                            agacaggTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                            <  1:526269/134‑1 (MQ=255)
                                                                cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                        <  2:506515/140‑1 (MQ=255)
                                                                cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                        <  2:226766/140‑1 (MQ=255)
                                                                 tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAAAAGGAGGTGCCATGATGAGGGAAAGAAAAAAACAAAAAGCAGAGAATaa                                                                       >  2:447874/1‑140 (MQ=255)
                                                                           atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGGTGGCCTGATGAGCGGAAGGAATAAACAAAAAGCAGTGAATAAATTTACagag                                                             >  2:1539988/1‑140 (MQ=255)
                                                                           atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCTAGAAAAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAGAAATAAAAAACAGCCAGTGAAAAAGATGACagag                                                             >  2:1043677/1‑140 (MQ=255)
                                                                           atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTTACACAAAAAAAAAAAATGGGGGTGGCCTGATGGGGGAAAGGAAAAAACAAAAAGGAGAGAAAAAAATGACagaa                                                             >  2:201843/1‑139 (MQ=255)
                                                                             atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                           <  1:54178/140‑1 (MQ=255)
                                                                             atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGACAAAAACAAAAAAAGGGGGTGGCATGGTGAGGGGAAGTAATAAAGAAAAAGCAGTGAAAAAATTTACAaagaa                                                           >  2:188851/1‑139 (MQ=255)
                                                                             atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGAAAAAAAAAAAAAAAGGAGGTGGCATGATGAGGGAAAGAAATAAAAAAAAAGCAGTGAATAAATTGAAAGAGAt                                                           >  2:1016247/1‑140 (MQ=255)
                                                                                 aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAAACAAAAAAAAAAAATAGGGTTGCCATGGTGGGCGAAAGGAAAAAGAAAAAAGAAGGGAATAAATTTAAAGAGATTGtt                                                       >  2:1307731/1‑139 (MQ=255)
                                                                                      ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCATGATGAGCGGAAATAAAAAACAAAAGACAAGGAAAAAAATGACAGAGATTGTTGCTaa                                                  >  2:1179634/1‑140 (MQ=255)
                                                                                       gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCCTGATGAGGGAAAATAATAAACAACAAGCAGGGAAAAAAATGACAGAGATTGTCGCTAAc                                                 >  2:182654/1‑140 (MQ=255)
                                                                                            aaaTATAAACCCACATTTTTAGTGGTAGTTTGTGGGGGTGATTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAc                                            <  1:188851/140‑1 (MQ=255)
                                                                                                 aaaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAGAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                       <  1:1043677/139‑1 (MQ=255)
                                                                                                                 gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACCGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                       <  2:922406/140‑1 (MQ=255)
                                                                                                                         ttGTGGGGGTGATTTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGAGGAGCAAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCtgatg               <  2:823553/140‑1 (MQ=255)
                                                                                                                         ttGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg               <  2:461983/140‑1 (MQ=255)
                                                                                                                                   aaaTTTTTCAAAACAAATAAAAAAACGGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa     <  2:152938/139‑1 (MQ=255)
                                                                                                                                      tttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa  >  1:386169/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                      
GCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAA  >  NC_000913/3206296‑3206569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑