| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,556,207 | 0 | T | C | 22.9% | 100.9 / 11.0 | 48 | S215G (AGT→GGT) | rtcA | RNA 3'‑terminal phosphate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (35/2); new base C (0/11); total (35/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAC > NC_000913/3556068‑3556339 | accTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑Ct > 2:878064/1‑140 (MQ=255) ccGGCATTGCCGCGCTTTCCACCGACGACAAAAATGCTTTGGGTGATATTTTCACTTCCACCTCCAGGCGAGCCGGTTTTCCCCGGCCCAGGGTCGCGCGGCAGGTTATGAATTTTGTGTTCAGGCGGGGAAAAA‑CTccc < 2:508830/139‑1 (MQ=255) aCTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCa > 1:874717/1‑140 (MQ=255) aCTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCa > 2:67014/1‑140 (MQ=255) aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt > 1:1295007/1‑140 (MQ=255) aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt > 2:938090/1‑140 (MQ=255) gcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCg > 1:61277/1‑140 (MQ=255) ccGACGAAAAAAAAGCGTTCGGTAATTTTTTCACTTTCGACTTCAGGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACg < 2:1401890/140‑1 (MQ=255) gacgaaAAAAAGGCGTTGGGTTATTTTTTCTCTTTCGTCTTCAAGCGAGCCGGTATTCCCCGCCCCCGGGTCGGGGGGCGGTTTATAAATTTTCTGTTAATGCGGGGAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCt < 2:61277/140‑1 (MQ=255) acgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTc > 2:537471/1‑140 (MQ=255) acAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGc > 1:1186492/1‑140 (MQ=255) acAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGc > 1:1589543/1‑140 (MQ=255) tttCGGTGATTTTTTCTCTTCCGACTTCAAGCGAGACGTTATTCCCCGCCCCCGGGCCGCGGGGCGGGTTATAAATTTTCTGTCCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcgc < 2:439495/139‑1 (MQ=255) ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg > 1:512022/1‑140 (MQ=255) tCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg > 2:216119/1‑140 (MQ=255) ggtatttttttCACTTTCGACTTCAAGAGAGACGTTATTCCCCGGCCCCTGGCCGCGCGGCGGTTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca < 2:879071/133‑1 (MQ=255) atttttCACTTTCGACTTCAAGGGAGGGGTTTTCCCCCGGCCCGTGGTGGGGGGGGAGGTTATGAATTTTCTTTTCAAGCGGGGAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa < 2:333820/138‑1 (MQ=255) tCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa > 1:699677/1‑140 (MQ=255) tttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa > 1:1617196/1‑140 (MQ=255) tttCGACTCCAGGCGGGACGTTATCCCCCGGCCCCTGGCCGCGCGGCAGTTTATGAATTTTCTTTTCAAGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa < 2:824198/140‑1 (MQ=255) ttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa > 2:622538/1‑140 (MQ=255) tCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCACCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTGATAGAAc < 1:287260/140‑1 (MQ=255) tCGACTTAAAGCGAGACGGTATTCCCCGCCCCCGGGCCGCGGGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc < 2:451987/140‑1 (MQ=255) ccGACTTAAAGGGAGCCGTTATCCCCCGGCCCCTGGTCGCGCGGCAGTTTATAATTATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc < 2:1617196/139‑1 (MQ=255) cAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCGGGTTATGAATATTCTGTTCATGCAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCa < 1:878064/140‑1 (MQ=255) aaaGCGAGCGGGTTTCCCCCGGCCCCTGGTCGCGGGGCAGTTTATGAATATTCTGTTCATG‑AGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCa < 2:1186492/139‑1 (MQ=255) aaGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCAc > 1:401898/1‑140 (MQ=255) gagaCGGTATTCCCCGGCCCTTGGCCGGGGGGCGGTTTATAAATATTTTGTCCAGGCAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCAt < 2:228364/140‑1 (MQ=255) gttttcccccGCCCCCTGGCCGCGGGGCGGTTTATAAATTTTCTGTTAATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGca < 2:699677/133‑1 (MQ=255) gttATTCCCCGGCCCCGGGCCGCGCGGCAGGTTATAAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGca < 2:1295007/138‑1 (MQ=255) tATTCCCCGGCCCCGGGTCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGcaca < 2:622428/140‑1 (MQ=255) cccGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGtt > 1:234965/1‑140 (MQ=255) ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCcgc > 1:966782/1‑140 (MQ=255) ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCcgc > 2:1356536/1‑140 (MQ=255) ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCcgc > 1:56109/1‑140 (MQ=255) ccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCgcgc < 1:537471/140‑1 (MQ=255) tGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTc > 1:851374/1‑140 (MQ=255) ggTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCg < 1:622538/140‑1 (MQ=255) gTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGc > 1:413987/1‑140 (MQ=255) cgcgGCAGTTTATAAATTTTCTGTTCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg < 2:966782/140‑1 (MQ=255) agattATAAATTTTCTGTTCATGCAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCaa < 2:874717/137‑1 (MQ=255) ggTTATGAATTTTCTTTTCA‑GGAGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAg < 2:512022/140‑1 (MQ=255) aTGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtgt > 1:343273/1‑140 (MQ=255) taaaTTTTCTGTACAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt < 1:67014/138‑1 (MQ=255) atatTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTaaa > 2:1004494/1‑140 (MQ=255) ttCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa > 2:1702798/1‑140 (MQ=255) ttCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa > 2:1152379/1‑140 (MQ=255) tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt > 1:1014732/1‑140 (MQ=255) tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt > 1:732459/1‑140 (MQ=255) cTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATg > 2:1123116/1‑140 (MQ=255) tGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc > 2:207667/1‑140 (MQ=255) tGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc > 1:531998/1‑140 (MQ=255) ttCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc > 1:1683693/1‑140 (MQ=255) ttCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa > 1:36946/1‑140 (MQ=255) ttCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa < 2:1518211/140‑1 (MQ=255) aTGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg > 1:859431/1‑140 (MQ=255) tGCAGGAAAACC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg < 1:1034623/140‑1 (MQ=255) gCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc > 1:519820/1‑140 (MQ=255) gCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc > 1:1518211/1‑140 (MQ=255) gaaaaac‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgaga < 2:519820/138‑1 (MQ=255) gAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc > 1:406396/1‑140 (MQ=255) | ACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACT‑‑TCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAC > NC_000913/3556068‑3556339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |