Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,938,402	G→T	D252Y (GAC→TAC)	pykA →	pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,938,402	0	G	T	100.0%	305.5 / NA	87	D252Y (GAC→TAC)	pykA	pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (50/37);  total (50/37)

TGCCCGTCGCCTGGCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAC  >  NC_000913/1938266‑1938538                                                                                                                                         |                                                                                                                                         tGCCCGTCGCCTGGCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTAcc                                                                                                                                       >  1:1095766/1‑140 (MQ=255) tGCCCGTCGCCTGGCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTAcc                                                                                                                                       >  1:1608949/1‑140 (MQ=255) tGCCCGTCGCCTGGCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTAcc                                                                                                                                       >  2:1005096/1‑140 (MQ=255)  gCCCGTCGCCTGGAACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCt                                                                                                                                      <  1:1174721/140‑1 (MQ=255)    ccGTCGCCTGGCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCg                                                                                                                                    <  2:1608949/140‑1 (MQ=255)             ggCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGaaa                                                                                                                           >  1:480488/1‑140 (MQ=255)              gCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAAt                                                                                                                          >  1:204785/1‑140 (MQ=255)              gCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAAt                                                                                                                          >  1:877310/1‑140 (MQ=255)              gCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAAt                                                                                                                          >  2:306109/1‑140 (MQ=255)              gCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAAt                                                                                                                          >  2:1089768/1‑140 (MQ=255)                 cgcgATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAGGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTgg                                                                                                                       <  2:809895/140‑1 (MQ=255)                 cgcgATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTgg                                                                                                                       <  1:1089768/140‑1 (MQ=255)                 cgcgATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTgg                                                                                                                       <  1:306109/140‑1 (MQ=255)                    gATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGa                                                                                                                    <  1:1005096/140‑1 (MQ=255)                     aTGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGAc                                                                                                                   >  2:16096/1‑140 (MQ=255)                            aTGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGc                                                                                                                      <  1:1237522/130‑1 (MQ=255)                            aTGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGc                                                                                                                      >  2:1237522/1‑130 (MQ=255)                             tgtgATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACt                                                                                                           <  2:19683/140‑1 (MQ=255)                             tgtgATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACt                                                                                                           <  2:995977/140‑1 (MQ=255)                              gtgATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTg                                                                                                          >  1:1058970/1‑140 (MQ=255)                                     gAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCa                                                                                                   >  1:640265/1‑140 (MQ=255)                                           tgttgCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGa                                                                                             <  2:1495381/140‑1 (MQ=255)                                           tgttgCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGa                                                                                             <  2:1177031/140‑1 (MQ=255)                                           tgttgCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGa                                                                                             <  1:41135/140‑1 (MQ=255)                                           tgttgCCAAGGTTGAACGGGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCATCTGACGTGGTAATGGTTGCACGTGGCTCCCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGa                                                                                             <  2:582907/140‑1 (MQ=255)                                                ccAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCg                                                                                        >  1:1634432/1‑140 (MQ=255)                                                             tGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTg                                                                           <  2:1100355/140‑1 (MQ=255)                                                               cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcg                                                                         >  1:1627097/1‑140 (MQ=255)                                                               cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcg                                                                         >  1:612028/1‑140 (MQ=255)                                                               cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcg                                                                         >  1:1372429/1‑140 (MQ=255)                                                               cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcg                                                                         >  2:489088/1‑140 (MQ=255)                                                               cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcg                                                                         >  1:95308/1‑140 (MQ=255)                                                               cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTgcg                                                                         >  1:1278272/1‑140 (MQ=255)                                                                ggAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcgc                                                                        <  1:16096/140‑1 (MQ=255)                                                                   aGCCGTTTGCAGGCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAAATGGCGACCCGGGACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTc                                                                     >  2:1642394/1‑140 (MQ=255)                                                                            cAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAAcc                                                            >  1:1697781/1‑140 (MQ=255)                                                                              gCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGa                                                          >  2:19227/1‑140 (MQ=255)                                                                               ccAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAg                                                         >  1:977963/1‑140 (MQ=255)                                                                               ccAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGGCAGCTAAACCGAg                                                         >  2:1026229/1‑140 (MQ=255)                                                                                   gATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGt                                                     <  2:1095766/140‑1 (MQ=255)                                                                                          tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                              <  2:480488/140‑1 (MQ=255)                                                                                          tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                              <  2:204785/140‑1 (MQ=255)                                                                                          tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                              <  2:877310/140‑1 (MQ=255)                                                                                            gATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGc                                            <  2:989368/140‑1 (MQ=255)                                                                                            gATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGc                                            <  2:208300/140‑1 (MQ=255)                                                                                            gATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGc                                            >  2:194559/1‑140 (MQ=255)                                                                                             aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACgg                                             >  2:1430042/1‑138 (MQ=255)                                                                                             aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACgg                                             <  1:1430042/138‑1 (MQ=255)                                                                                             aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                           >  1:709784/1‑140 (MQ=255)                                                                                             aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                           >  1:332470/1‑140 (MQ=255)                                                                                             aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                           >  2:230532/1‑140 (MQ=255)                                                                                             aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                           >  1:570122/1‑140 (MQ=255)                                                                                              tGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGa                                          >  1:506696/1‑140 (MQ=255)                                                                                               gACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAc                                         <  1:489088/140‑1 (MQ=255)                                                                                               gACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAc                                         <  1:194559/140‑1 (MQ=255)                                                                                                 catcatCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAccc                                       <  2:640265/140‑1 (MQ=255)                                                                                                 catcatCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAccc                                       >  1:1459926/1‑140 (MQ=255)                                                                                                  atcatcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCa                                      <  1:1718363/140‑1 (MQ=255)                                                                                                  atcatcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCa                                      >  1:107702/1‑140 (MQ=255)                                                                                                     atcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgat                                   <  2:1627097/140‑1 (MQ=255)                                                                                                      tcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATTACGGCGACCCAgatg                                  >  2:1182821/1‑140 (MQ=255)                                                                                                      tcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgatg                                  >  2:1135996/1‑140 (MQ=255)                                                                                                        cTCGCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgatgat                                <  2:570122/140‑1 (MQ=255)                                                                                                           gCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGa                             >  2:433481/1‑140 (MQ=255)                                                                                                           gCCTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGa                             <  1:433481/140‑1 (MQ=255)                                                                                                            ccTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                            >  2:1495375/1‑140 (MQ=255)                                                                                                            ccTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                            >  2:968378/1‑140 (MQ=255)                                                                                                            ccTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                            <  2:977963/140‑1 (MQ=255)                                                                                                            ccTCTGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                            >  2:878170/1‑140 (MQ=255)                                                                                                               ctGACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCa                         >  1:1186500/1‑140 (MQ=255)                                                                                                                 gACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                       <  1:1495375/140‑1 (MQ=255)                                                                                                                 gACGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                       <  2:1278272/140‑1 (MQ=255)                                                                                                                  aCGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATg                      >  2:1316693/1‑140 (MQ=255)                                                                                                                  aCGTGGTAATGGTTGCACGTGGCTACCTCGGTGTGGAAAATGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATg                      <  1:1642394/140‑1 (MQ=255)                                                                                                                       gTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                       <  1:618078/134‑1 (MQ=255)                                                                                                                       gTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                       >  2:618078/1‑134 (MQ=255)                                                                                                                       gTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTAc                 >  2:57988/1‑140 (MQ=255)                                                                                                                       gTAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTAc                 >  1:1298707/1‑140 (MQ=255)                                                                                                                        tAATGGTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACt                <  2:107702/140‑1 (MQ=255)                                                                                                                             gTTGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAccc           >  1:1039965/1‑140 (MQ=255)                                                                                                                              ttGCACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCg          <  2:737004/140‑1 (MQ=255)                                                                                                                                 cACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg       >  2:54589/1‑140 (MQ=255)                                                                                                                                 cACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg       <  1:1316693/140‑1 (MQ=255)                                                                                                                                 cACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg       >  1:292341/1‑140 (MQ=255)                                                                                                                                 cACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg       >  1:576621/1‑140 (MQ=255)                                                                                                                                 cACGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg       >  1:744276/1‑140 (MQ=255)                                                                                                                                  aCGTGGCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGc      >  1:628276/1‑140 (MQ=255)                                                                                                                                      ggCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAc  >  1:1671092/1‑140 (MQ=255)                                                                                                                                      ggCTACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAc  <  2:95308/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGCCCGTCGCCTGGCACGCGATGCAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAC  >  NC_000913/1938266‑1938538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑