Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	980,779	C→A	A1485E (GCG→GAG)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	980,779	0	C	A	100.0%	328.5 / NA	92	A1485E (GCG→GAG)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (50/42);  total (50/42)

CCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGT  >  NC_000913/980642‑980915                                                                                                                                          |                                                                                                                                         cccGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGgaga                                                                                                                                        >  1:1341713/1‑140 (MQ=255)     gagaAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTa                                                                                                                                    >  1:335508/1‑140 (MQ=255)           ggCACCACCTATAAACTGGTGCGTAAAGTC‑TCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGtt                                                                                                                                     <  1:1411778/132‑1 (MQ=255)           ggCACCACCTATAAACTGGTGCGTAAAGTC‑TCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGtt                                                                                                                                     >  2:1411778/1‑132 (MQ=255)               ccaccTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAggc                                                                                                                          >  1:1031022/1‑140 (MQ=255)                caccTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAggcg                                                                                                                         >  1:1083727/1‑140 (MQ=255)                     ataAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGgcagc                                                                                                                    >  2:51524/1‑140 (MQ=255)                      taAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGgcagca                                                                                                                   <  2:1383264/140‑1 (MQ=255)                        aaCTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAAt                                                                                                                 <  2:739975/140‑1 (MQ=255)                          cTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAggc                                                                                                                          >  1:132669/1‑129 (MQ=255)                          cTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAggc                                                                                                                          <  2:132669/129‑1 (MQ=255)                           tGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgcc                                                                                                              <  2:1315960/140‑1 (MQ=255)                            ggTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccg                                                                                                             >  1:855451/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCTAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  2:929232/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  2:1283831/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  2:1423526/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  2:1279560/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  2:1146167/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  2:1468037/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  2:337572/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  1:785167/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  1:535872/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  2:1058996/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  1:332424/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  1:165256/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  1:1607303/1‑140 (MQ=255)                             gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgc                                                                                                            >  1:1251002/1‑140 (MQ=255)                              tGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATgccgcc                                                                                                           >  1:142723/1‑140 (MQ=255)                                cGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCctt                                                                                                         >  2:1053320/1‑140 (MQ=255)                                  tAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCctttt                                                                                                       >  1:1297947/1‑140 (MQ=255)                                  tAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCctttt                                                                                                       >  2:628982/1‑140 (MQ=255)                                        cTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCttttt                                                                                                 >  1:1645794/1‑140 (MQ=255)                                            cAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGa                                                                                             <  2:487116/140‑1 (MQ=255)                                                aTACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAAc                                                                                         <  2:142723/140‑1 (MQ=255)                                                 tACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACt                                                                                        >  2:1422297/1‑140 (MQ=255)                                                 tACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACt                                                                                        >  1:1571935/1‑140 (MQ=255)                                                 tACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACt                                                                                        >  1:148480/1‑140 (MQ=255)                                                 tACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACt                                                                                        >  2:319232/1‑140 (MQ=255)                                                 tACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACt                                                                                        >  2:245905/1‑140 (MQ=255)                                                  aCCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTc                                                                                       <  1:1423526/140‑1 (MQ=255)                                                   ccGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTcc                                                                                      >  1:351883/1‑140 (MQ=255)                                                     gAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGt                                                                                    >  2:709456/1‑140 (MQ=255)                                                      aaCACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGtt                                                                                   >  2:10154/1‑140 (MQ=255)                                                      aaCACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGtt                                                                                   >  2:62910/1‑140 (MQ=255)                                                          cGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTg                                                                               <  1:62910/140‑1 (MQ=255)                                                          cGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTg                                                                               <  1:1053320/140‑1 (MQ=255)                                                          cGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTg                                                                               <  2:855451/140‑1 (MQ=255)                                                          cGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTg                                                                               <  2:1083727/140‑1 (MQ=255)                                                           gTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGc                                                                              >  2:801125/1‑140 (MQ=255)                                                            ttCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCa                                                                             <  2:335508/140‑1 (MQ=255)                                                             tCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCAc                                                                            >  1:246403/1‑140 (MQ=255)                                                                tGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTaa                                                                         <  1:1283831/140‑1 (MQ=255)                                                                tGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTaa                                                                         <  2:165256/140‑1 (MQ=255)                                                                   cgtcgGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTaaaaa                                                                      >  1:411030/1‑140 (MQ=255)                                                                    gtcgGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTaaaaaa                                                                     >  2:1260439/1‑140 (MQ=255)                                                                     tcgGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTaaaaaat                                                                    >  2:273398/1‑139 (MQ=255)                                                                     tcgGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAg                                                                    <  1:1368023/140‑1 (MQ=255)                                                                         ccTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGc                                                                >  1:1464332/1‑140 (MQ=255)                                                                         ccTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGc                                                                >  2:891012/1‑140 (MQ=255)                                                                             cGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACAt                                                            >  2:1357156/1‑140 (MQ=255)                                                                                   tttGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGTGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGgc                                                      >  2:1409824/1‑140 (MQ=255)                                                                                        gccgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCt                                                 <  2:433400/140‑1 (MQ=255)                                                                                        gccgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCt                                                 <  2:1231412/140‑1 (MQ=255)                                                                                        gccgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCt                                                 <  1:891012/140‑1 (MQ=255)                                                                                                  ccTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTaaa                                       >  2:635438/1‑140 (MQ=255)                                                                                                  ccTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTaaa                                       <  2:351883/140‑1 (MQ=255)                                                                                                  ccTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTaaa                                       <  2:246403/140‑1 (MQ=255)                                                                                                   cTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAAc                                      <  1:51524/140‑1 (MQ=255)                                                                                                     gAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAGCTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAActt                                    <  1:709456/140‑1 (MQ=255)                                                                                                      aaaCGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAActtc                                   >  1:1066832/1‑140 (MQ=255)                                                                                                       aaCGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTTGCACATTACGGCGCGCTTTTCTTTAAActtct                                  <  1:1058996/140‑1 (MQ=255)                                                                                                       aaCGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAActtct                                  <  2:1031022/140‑1 (MQ=255)                                                                                                       aaCGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAActtct                                  <  2:1231532/140‑1 (MQ=255)                                                                                                       aaCGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAActtct                                  <  2:251318/140‑1 (MQ=255)                                                                                                       aaCGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAActtct                                  <  1:1146167/140‑1 (MQ=255)                                                                                                       aaCGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAActtct                                  <  2:380919/140‑1 (MQ=255)                                                                                                       aaCGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAActtct                                  <  2:535872/140‑1 (MQ=255)                                                                                                            ttCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCTTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGACACATTACGGCGCGCTTTTCTTTAAACTTCTTTACa                             <  1:1422297/140‑1 (MQ=255)                                                                                                            ttCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACa                             <  2:1464332/140‑1 (MQ=255)                                                                                                            ttCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACa                             <  1:1357156/140‑1 (MQ=255)                                                                                                                 ggAACTGACGAAGCGCCTTCTCAGGAGTGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAgg                        <  1:1409824/140‑1 (MQ=255)                                                                                                                      tGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATg                   >  1:505851/1‑140 (MQ=255)                                                                                                                         cGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTaa                >  1:41780/1‑140 (MQ=255)                                                                                                                           aaGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTaaaa              >  2:1466057/1‑140 (MQ=255)                                                                                                                             gcgcCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAAc            >  1:677651/1‑140 (MQ=255)                                                                                                                                    ctcAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCgg     <  1:337572/140‑1 (MQ=255)                                                                                                                                    ctcAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCgg     <  2:1251002/140‑1 (MQ=255)                                                                                                                                    ctcAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCgg     <  2:1607303/140‑1 (MQ=255)                                                                                                                                    ctcAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCgg     <  2:148480/140‑1 (MQ=255)                                                                                                                                    ctcAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCgg     <  1:1260439/140‑1 (MQ=255)                                                                                                                                    ctcAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCgg     <  2:1259060/140‑1 (MQ=255)                                                                                                                                    ctcAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTAGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCgg     <  1:591750/140‑1 (MQ=255)                                                                                                                                       aGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGt  <  1:801125/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGT  >  NC_000913/980642‑980915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑