Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,114	0	G	T	25.3%	200.9 / 13.3	75	intergenic (‑319/+71)	ybgD/gltA	putative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (49/7);  new base T (0/19);  total (49/26)
Fisher's exact test for biased strand distribution p-value = 2.29e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAA  >  NC_000913/752976‑753252                                                                                                                                           |                                                                                                                                            cATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTgtgt                                                                                                                                            >  2:42679/1‑140 (MQ=255) cATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTgtgt                                                                                                                                            >  1:165870/1‑140 (MQ=255)   ttttCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGttt                                                                                                                                          >  2:988810/1‑140 (MQ=255)       cATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGc                                                                                                                                      <  1:1277589/140‑1 (MQ=255)          aGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCata                                                                                                                                   <  1:1006576/140‑1 (MQ=255)               tataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaa                                                                                                                              >  1:999247/1‑140 (MQ=255)                ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaaa                                                                                                                             >  1:977847/1‑140 (MQ=255)                ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGATAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaaa                                                                                                                             >  1:1562700/1‑140 (MQ=255)                      gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAc‑c                                                                                                                      >  2:1488010/1‑140 (MQ=255)                      gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAc‑c                                                                                                                      >  1:659755/1‑140 (MQ=255)                      gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAc‑c                                                                                                                      >  1:186118/1‑140 (MQ=255)                      gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAc‑c                                                                                                                      >  2:248832/1‑140 (MQ=255)                       cGACTACCATGAATTTTTTAATTGAAAGTATTGGGTTGCTGATATTTTGACCTTTTTTATTTTTTTTAAATATCTATATAGGTCTGTTAATGATTTTTTTTTTTACAATTTTTTGTTTTTAGGCATATAAAAACCAAc‑cc                                                                                                                     <  2:87799/140‑1 (MQ=255)                       cGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAc‑cc                                                                                                                     >  2:1631603/1‑140 (MQ=255)                        gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCg                                                                                                                    >  1:1371083/1‑140 (MQ=255)                        gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCg                                                                                                                    >  1:1236079/1‑140 (MQ=255)                                tGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGa                                                                                                            >  1:1374934/1‑140 (MQ=255)                                tGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGa                                                                                                            >  2:1580341/1‑140 (MQ=255)                                tGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGa                                                                                                            >  1:87799/1‑140 (MQ=255)                                   attttttAATTAAAAGTTTTGGTTTGCTAATATTTTGAGTTTTTTTTTTTTTTTTAAATATTTATATAGTTTTTTTAATGTATTTTTTTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCAAC‑CCGCCATATGAAcg                                                                                                         <  2:986662/138‑1 (MQ=255)                                      ttttAATTAAAAGTATTGGGTTGTTGATAATTTGAGTTTTTCTATTTTTTTTAAATATCTATATAGGTCTTTTAATGGTTTTTTTTTTTAAAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAAcggcg                                                                                                      <  2:1444628/140‑1 (MQ=255)                                       tttAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAAcggcgg                                                                                                     >  2:1516565/1‑140 (MQ=255)                                               aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTaaaaa                                                                                             >  2:1334289/1‑139 (MQ=255)                                               aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTTAAat                                                                                             >  2:1478815/1‑140 (MQ=255)                                               aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAat                                                                                             >  1:1445489/1‑140 (MQ=255)                                               aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGGTaaaaa                                                                                             >  1:395476/1‑139 (MQ=255)                                                 aGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAatat                                                                                           >  2:1077055/1‑140 (MQ=255)                                                         gTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTTTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATAAAAAATTCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACt                                                                                   <  1:782284/140‑1 (MQ=255)                                                         gTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACt                                                                                   >  2:701504/1‑140 (MQ=255)                                                         gTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACt                                                                                   <  1:701504/140‑1 (MQ=255)                                                             cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                               >  1:887564/1‑140 (MQ=255)                                                             cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                               >  1:1035771/1‑140 (MQ=255)                                                                 tAATTTGAGCTTTTCTATTTTTTTTAAATATCTATATAGTTCTTTTAATGGTTTTTATTTTTACATTTTTTTGTTTTTAGGCAAAAAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATc                                                                           <  2:186118/140‑1 (MQ=255)                                                                   ttttGAGTTTTTTTTTTTTTTTTAAATTTTTATATAGGTTTGTTAATGTTTTTTTTTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCaa                                                                         <  2:914809/139‑1 (MQ=255)                                                                          cTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTaa                                                                  >  1:1196231/1‑140 (MQ=255)                                                                             ttCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGc                                                               >  2:777731/1‑140 (MQ=255)                                                                             ttCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAAAATTTACAACTTAGCAAACAACCATTAACGc                                                               >  1:1654341/1‑140 (MQ=255)                                                                                  tttttttaAAATTTTTATAAAGGTTTGTTAATGTTTTTTTTTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCACC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGat                                                          <  2:977847/137‑1 (MQ=255)                                                                                  ttCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAAAATTTACAACTTAGCAATCAACCATTAACGCTTGat                                                          >  1:602164/1‑140 (MQ=255)                                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATccc                                                     >  1:1326653/1‑138 (MQ=255)                                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                     >  2:295471/1‑140 (MQ=255)                                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                     >  2:1189752/1‑140 (MQ=255)                                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                     >  2:794565/1‑140 (MQ=255)                                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                     >  1:659400/1‑140 (MQ=255)                                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                     >  2:1351623/1‑140 (MQ=255)                                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                     >  1:399640/1‑140 (MQ=255)                                                                                       tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                     >  2:1503984/1‑140 (MQ=255)                                                                                               tCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATTCCTTTTtaaa                                             >  2:942103/1‑139 (MQ=255)                                                                                               tCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAg                                             >  1:1227208/1‑140 (MQ=255)                                                                                                    ttaGGTCTGTGAATGGATTTGATTTTTATAATTGTGTGTGGTTAAACATA‑AAAAAACAACGCCGCCATATGAACGGCGGGTTAAAATATTTAGAACTTAGCAATCAACCATTGACGGTTGGTATCGCTTTTAAAGTcgcg                                        <  2:1236079/139‑1 (MQ=255)                                                                                                     tgggTTTGTTAATGGTTTTTTTTTTTAAATTTTTTTGTTTTTGGGCATAAAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                       <  1:490426/138‑1 (MQ=255)                                                                                                     taGGTTTGTTAATGTATTTTTTTTTTACAATTTTTTGTTTTTAGGCAAAAAAAAACCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                       <  2:247310/140‑1 (MQ=255)                                                                                                        gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTTCAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGgttt                                    >  2:1475776/1‑140 (MQ=255)                                                                                                        gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                    >  2:487950/1‑140 (MQ=255)                                                                                                        gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                    >  1:194376/1‑140 (MQ=255)                                                                                                        gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                    >  2:1313015/1‑140 (MQ=255)                                                                                                        gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGgttt                                    >  1:815515/1‑140 (MQ=255)                                                                                                           tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                 >  1:1603945/1‑140 (MQ=255)                                                                                                           tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAAACATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                 >  1:247310/1‑140 (MQ=255)                                                                                                            tttAATGGTTTTTTTTTTTACATTTTTTTGTTTTTAGGCATAAAAAAATCACC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                <  2:172173/139‑1 (MQ=255)                                                                                                            tttAATGGTTTTTTTTTTTACAATTTTTTGTTTTTAGGAATAAAAAAATCACC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                <  2:659755/139‑1 (MQ=255)                                                                                                            tttAATGGTTTTTATTTTTACATTTTTTTGTTTTTAGGAATAAAAAAACCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                <  2:165870/139‑1 (MQ=255)                                                                                                            tttAATGGTTTTTATTTTTACAATTTTTTGTTTTTAGGCATAAAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATAGCTTTTAAAGTCGCGTTTTTCat                                <  1:1509193/139‑1 (MQ=255)                                                                                                            tttAATGGATTTTATTTTTAAATTTTTTTGTTTTTAGACATAAAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                <  2:1604492/139‑1 (MQ=255)                                                                                                            tttAATGGATTTTATTTTAACAATTTTTTGTTTTTAGGAATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                <  1:248832/139‑1 (MQ=255)                                                                                                            ttaattGGTTTTTATTTTTAAATTTTTTTGTTTTTAGACATAAAAAAACCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                <  2:294485/135‑1 (MQ=255)                                                                                                            ttaaaTGGATTTTTTTTTTACAATTTTTTGTGTTTAGACATAAAAAAATCACC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                <  1:1663687/137‑1 (MQ=255)                                                                                                            gTTAATGGTTTTTATTTTTACAATTTTTTGTTTTTAGGCATAAAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                <  1:925731/140‑1 (MQ=255)                                                                                                             ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                               >  1:909540/1‑140 (MQ=255)                                                                                                             ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                               >  1:278755/1‑140 (MQ=255)                                                                                                              tAATGGATTTTTTTTTTAAAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCatat                              <  1:1407067/140‑1 (MQ=255)                                                                                                               aatgttttttttttttAAATTTTTTTGTTTTTGGGAATAAAAAAACCACC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                             <  2:399640/134‑1 (MQ=255)                                                                                                               aaTGGATTTTTTTTTTACATTTTTTTGTTTTTAGGCATAAAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                             <  1:1631603/140‑1 (MQ=255)                                                                                                               aaTGGATTTTATTTTTACAATTTTTTGTTTTTAGGCATAAAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                             <  1:42679/140‑1 (MQ=255)                                                                                                               aaTGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                             <  1:1351623/140‑1 (MQ=255)                                                                                                                  ggATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTg                          >  1:849073/1‑140 (MQ=255)                                                                                                                  ggATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGGTAAAATTTTTATAACTTAGCAAGAAAACAATAAGGCTTGATATCGCTTTTAAAGTCCCGTTTTTCTTTTCCtt                          >  2:840507/1‑139 (MQ=255)                                                                                                                   gATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGt                         >  2:13531/1‑140 (MQ=255)                                                                                                                                   ttttttGTTTTTAGGCATAAAAAAATCACC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGa         <  2:887564/140‑1 (MQ=255)                                                                                                                                        tgttTTTAGGAATAAAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc    <  2:1374934/140‑1 (MQ=255)                                                                                                                                        tgttTTTAGGAATAAAAAAACCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc    <  2:194376/140‑1 (MQ=255)                                                                                                                                        tgtgTTTAGGCATAAAAAAATCACC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc    <  1:1488010/140‑1 (MQ=255)                                                                                                                                        tgtgTTTAGGAAAAAAAAACCCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTATATACAGCTGACGCGGACGGGc    <  2:1227208/140‑1 (MQ=255)                                                                                                                                          tgtTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCaa  <  1:777731/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                            CATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAC‑CCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAA  >  NC_000913/752976‑753252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑