BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	352,810	C→A	L35L (CTC→CTA)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	352,810	0	C	A	99.1%	385.0 / ‑3.2	107	L35L (CTC→CTA)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (61/45); total (61/46)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 108 total aligned reads displayed.

GGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAG  >  NC_000913/352676‑352943

ggTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATca                                                                                                                                  >  1:755819/1‑140 (MQ=255)

ggTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATca                                                                                                                                  >  1:1818621/1‑140 (MQ=255)

        gTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATc                                                                                                                          <  1:1487888/140‑1 (MQ=255)

         tAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATcc                                                                                                                         >  1:1095564/1‑140 (MQ=255)

                aaCAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGttt                                                                                                                  >  1:1623452/1‑140 (MQ=255)

                aaCAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGttt                                                                                                                  >  2:553495/1‑140 (MQ=255)

                aaCAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGtt                                                                                                                   <  1:974710/139‑1 (MQ=255)

                     gagaGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCg                                                                                                             >  1:670584/1‑140 (MQ=255)

                      agagCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGt                                                                                                            <  1:1292625/140‑1 (MQ=255)

                      agagCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGt                                                                                                            <  1:1908883/140‑1 (MQ=255)

                      agagCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGt                                                                                                            <  2:1243206/140‑1 (MQ=255)

                      agagCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGt                                                                                                            <  2:1173236/140‑1 (MQ=255)

                      agagCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGt                                                                                                            <  2:1167342/140‑1 (MQ=255)

                      agagCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGt                                                                                                            <  2:2036917/140‑1 (MQ=255)

                       gagCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTGTTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGtt                                                                                                           >  1:1548733/1‑140 (MQ=255)

                          cATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGt                                                                                                        >  1:1761365/1‑140 (MQ=255)

                               tGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTtgt                                                                                                   >  1:1927868/1‑140 (MQ=255)

                                   ttttAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGtt                                                                                                           >  1:427054/1‑128 (MQ=255)

                                   ttttAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGtt                                                                                                           <  2:427054/128‑1 (MQ=255)

                                    tttAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAgg                                                                                              >  1:240952/1‑140 (MQ=255)

                                    tttAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAgg                                                                                              <  2:1927868/140‑1 (MQ=255)

                                     ttAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGc                                                                                             <  2:670584/140‑1 (MQ=255)

                                     ttAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGc                                                                                             >  2:1732303/1‑140 (MQ=255)

                                      tAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAggtc                                                                                            >  2:130408/1‑138 (MQ=255)

                                         cGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGaa                                                                                         >  2:217901/1‑140 (MQ=255)

                                          gAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAAc                                                                                        <  2:703635/140‑1 (MQ=255)

                                          gAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAAc                                                                                        <  2:108191/140‑1 (MQ=255)

                                           aaTTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAAcc                                                                                       <  2:1095564/140‑1 (MQ=255)

                                            aTTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCa                                                                                      <  1:1732303/140‑1 (MQ=255)

                                             ttttATCAGCGTTCGATTAGCGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGaa                                                                                         <  2:1808336/136‑1 (MQ=255)

                                                    aGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGc                                                                              <  1:1430131/140‑1 (MQ=255)

                                                      cGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCca                                                                            >  2:1858999/1‑140 (MQ=255)

                                                      cGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCca                                                                            <  1:1298513/140‑1 (MQ=255)

                                                      cGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCca                                                                            <  2:1166482/140‑1 (MQ=255)

                                                       gTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCcac                                                                           >  1:105237/1‑140 (MQ=255)

                                                       gTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCcac                                                                           >  2:1412484/1‑140 (MQ=255)

                                                       gTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCcac                                                                           >  1:1194063/1‑140 (MQ=255)

                                                        ttCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTtg                                                                                 <  2:465805/133‑1 (MQ=255)

                                                        ttCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTtg                                                                                 >  1:465805/1‑133 (MQ=255)

                                                        ttCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCcaca                                                                          >  1:1788093/1‑140 (MQ=255)

                                                        ttCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCcaca                                                                          <  2:240952/140‑1 (MQ=255)

                                                          cGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAAc                                                                        >  1:92441/1‑140 (MQ=255)

                                                                aCGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATc                                                                  >  2:1745305/1‑140 (MQ=255)

                                                                aCGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATc                                                                  >  1:6845/1‑140 (MQ=255)

                                                                     ccGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCg                                                             <  2:1903181/140‑1 (MQ=255)

                                                                     ccGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCg                                                             <  1:847511/140‑1 (MQ=255)

                                                                      cGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgc                                                            >  2:593272/1‑140 (MQ=255)

                                                                       ggAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGTCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgct                                                           >  2:648085/1‑140 (MQ=255)

                                                                        gAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGGCCTTTACGCAAGCTCTAGATCACAGCAATCCGCCTTTTGCCCGTTGTTTTTGTGAAGGCCGAACCAACTTGTGCCTCAACGCCATCGACCgctg                                                          <  2:46928/140‑1 (MQ=255)

                                                                        gAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctg                                                          <  2:1818621/140‑1 (MQ=255)

                                                                        gAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctg                                                          <  2:755819/140‑1 (MQ=255)

                                                                        gAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctg                                                          <  1:1745305/140‑1 (MQ=255)

                                                                         aGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctgg                                                         >  2:1854034/1‑140 (MQ=255)

                                                                          gCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctggc                                                        <  1:1412484/140‑1 (MQ=255)

                                                                          gCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctggc                                                        <  1:1593785/140‑1 (MQ=255)

                                                                              ttCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGga                                                    >  2:53710/1‑140 (MQ=255)

                                                                                cTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgc                                                            >  1:850499/1‑130 (MQ=255)

                                                                                cTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgc                                                            <  2:850499/130‑1 (MQ=255)

                                                                                cTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGgaga                                                  >  1:1131463/1‑140 (MQ=255)

                                                                                   ggCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAAc                                               <  1:593272/140‑1 (MQ=255)

                                                                                       gAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctg                                                          >  2:142314/1‑125 (MQ=255)

                                                                                       gAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctg                                                          <  1:142314/125‑1 (MQ=255)

                                                                                       gAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGcc                                           >  1:645838/1‑140 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctggctg                                                      <  1:1938346/128‑1 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctggctg                                                      >  1:303709/1‑128 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCgctggctg                                                      >  2:1938346/1‑128 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                          >  2:946657/1‑140 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                          >  2:1822358/1‑140 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                          >  2:1401416/1‑140 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                          >  1:1292634/1‑140 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                          >  1:1487398/1‑140 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                          >  2:1366318/1‑140 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                          >  1:1664682/1‑140 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                          >  1:1851887/1‑140 (MQ=255)

                                                                                        aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTAGTGCCACAACGCCATCGACCgctggctg                                                      <  2:303709/128‑1 (MQ=255)

                                                                                                  gCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAggcgctgg                                >  1:1090199/1‑140 (MQ=255)

                                                                                                  gCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAggcgctgg                                <  1:1401416/140‑1 (MQ=255)

                                                                                                     tATTGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCACCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAggcgctggcgc                             <  2:971699/140‑1 (MQ=255)

                                                                                                       ttGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGgcgctggcgctg                           >  2:1650882/1‑140 (MQ=255)

                                                                                                       ttGACTGGCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGgcgctggcgctg                           >  1:22220/1‑140 (MQ=255)

                                                                                                        tGACTGGCCGACGCCCTTTACGAAATCGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGa                          <  2:1194063/140‑1 (MQ=255)

                                                                                                             ggCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGcc                     <  2:2014172/140‑1 (MQ=255)

                                                                                                             ggCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGcc                     <  2:1292634/140‑1 (MQ=255)

                                                                                                              gCAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCg                    >  2:1643467/1‑140 (MQ=255)

                                                                                                               cAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGt                   >  2:1573016/1‑140 (MQ=255)

                                                                                                               cAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGt                   >  1:1882232/1‑140 (MQ=255)

                                                                                                               cAGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGt                   >  2:2020711/1‑140 (MQ=255)

                                                                                                                aGACGCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGtc                  <  2:92441/140‑1 (MQ=255)

                                                                                                                    gCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCtt              <  1:1900096/140‑1 (MQ=255)

                                                                                                                    gCCCTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCAGCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCtt              >  2:1377381/1‑140 (MQ=255)

                                                                                                                      ccTTTACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCg            <  2:1548733/140‑1 (MQ=255)

                                                                                                                        tttACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGa          >  1:75224/1‑140 (MQ=255)

                                                                                                                        tttACGCAAACGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGa          >  1:1228037/1‑140 (MQ=255)

                                                                                                                                aaCGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGaaa        >  2:54763/1‑134 (MQ=255)

                                                                                                                                aaCGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGaaa        <  1:54763/134‑1 (MQ=255)

                                                                                                                                aaCGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACagaag  >  1:769750/1‑140 (MQ=255)

                                                                                                                                aaCGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACagaag  >  1:1752609/1‑140 (MQ=255)

                                                                                                                                aaCGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACagaag  >  1:1623690/1‑140 (MQ=255)

                                                                                                                                aaCGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACagaag  >  2:785710/1‑140 (MQ=255)

                                                                                                                                aaCGCTAGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGAGCCACAACGCCATCGAACGCAGGCTGGAGAAACAGCCAGTGGCGCTGACGCTGATTGCCGTCTCTTCGGAAACagaaa  >  2:1894472/1‑139 (MQ=255)

GGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAG  >  NC_000913/352676‑352943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`