Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4440TA25.0% 88.9 / 10.5 44intergenic (‑188/‑19)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (3/30);  new base A (11/0);  total (14/30)
Fisher's exact test for biased strand distribution p-value = 4.75e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGC  >  NC_000913/3206305‑3206580
                                                                                                                                            |                                                                                                                                        
tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAAt                                                                                                                                          >  1:163408/1‑140 (MQ=255)
  ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAc                                                                                                                                        <  2:1206615/140‑1 (MQ=255)
       cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCATAcaaaaacaaaaa                                                                                                                                   >  1:1316883/1‑140 (MQ=255)
       cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACaaaaa                                                                                                                                   >  1:958355/1‑140 (MQ=255)
       cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACaaaaa                                                                                                                                   >  1:147689/1‑140 (MQ=255)
           gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTgg                                                                                                                               <  2:976836/140‑1 (MQ=255)
            ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGC‑AAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAaaaaaaaaaaaaagag                                                                                                                             >  1:1400740/1‑134 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                             <  1:918027/140‑1 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                             <  1:603331/140‑1 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                             <  1:499820/140‑1 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                             <  1:359518/140‑1 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                             <  1:296114/140‑1 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                             <  2:958355/140‑1 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                             <  2:147689/140‑1 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                             <  2:690227/140‑1 (MQ=255)
               tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                           <  1:434703/140‑1 (MQ=255)
                 gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGAGGTgg                                                                                                                         >  2:528174/1‑139 (MQ=255)
                 gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAAAGGAGGTGc                                                                                                                         >  1:982993/1‑140 (MQ=255)
                 gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGGGTTgt                                                                                                                         >  2:897845/1‑139 (MQ=255)
                     aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatg                                                                                                                     <  1:1475875/140‑1 (MQ=255)
                       tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgat                                                                                                                   <  1:81808/140‑1 (MQ=255)
                        attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgatg                                                                                                                  <  2:489444/140‑1 (MQ=255)
                             ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                             <  1:897845/140‑1 (MQ=255)
                             ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                             <  2:1250476/140‑1 (MQ=255)
                             ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                             <  1:1434334/140‑1 (MQ=255)
                                 tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCCTGATGAGAGGAAgg                                                                                                         >  2:1105006/1‑139 (MQ=255)
                                   ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCA‑ACAAAAAAAAAAAAATGGGGTGCCATGATGAGCGgaaaaaa                                                                                                       >  2:440847/1‑136 (MQ=255)
                                   ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGGggaaggaa                                                                                                       >  2:126389/1‑140 (MQ=255)
                                        gcgTTGATTCACGAACTGTTATTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAAAAAAAAAAATGGAGGTGTCCTCAATAGCGAAAAgaataaaa                                                                                                  >  2:576722/1‑138 (MQ=255)
                                              aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                            <  1:1279286/140‑1 (MQ=255)
                                                     aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                     <  1:528174/140‑1 (MQ=255)
                                                     aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                     <  2:1062993/140‑1 (MQ=255)
                                                        tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCCTGATGAGGGGAAATAATAAACAAAAAGCAGTggaaaa                                                                                  >  1:176379/1‑135 (MQ=255)
                                                         gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                 <  2:163408/140‑1 (MQ=255)
                                                                aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag                                                                          <  2:19620/140‑1 (MQ=255)
                                                                  atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GAATTTTTCTGACAAAAACAAAAAATGGGGGTGGCATGATGAGGGAAAAAAATAAAAAAAAAGCAGGGAAAAAATTGACagaa                                                                        >  2:378779/1‑139 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGGTGTCATGATGAGGGAAAGAAATAAACAACAAGCAGTGAATAAAATGACAgagaa                                                                      >  2:1288162/1‑139 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAAAAAAAAAAAAGGGGGTGGCATGAAGAGGGAAAAAAATAAAAAACAAGCAGGGAAAAAATTGAAAGAGAt                                                                      >  2:894682/1‑140 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAAAGGAGGTGGCATGATGAGCGAAAAGAAAAAAAAAAAAGCAAGGAAAAAATTGACAaagaa                                                                      >  2:488194/1‑139 (MQ=255)
                                                                           gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                               <  1:378779/140‑1 (MQ=255)
                                                                                aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                          <  2:69181/140‑1 (MQ=255)
                                                                                        aaaaaCCACATTTTTAGTGGTAGTTTGTGGGGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTCGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                                  <  1:1105006/139‑1 (MQ=255)
                                                                                                    tttAGTGGTATTTTGTGGCGGTA‑ATTTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAccc                                      <  2:77876/140‑1 (MQ=255)
                                                                                                    tttAGTGGTAGTTTGGGGCGTTG‑AATTTTTCAAGACAAATACAAAAGCTGGAGTTGCCATGATGAGCAAAATTAATAACCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCCCTAACTTTACCGCCATGATTTCTAccc                                      <  2:439067/140‑1 (MQ=255)
                                                                                                    tttAGTGGTAGTTTGGGGCGGTG‑ATTTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCGGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAccc                                      <  2:44086/140‑1 (MQ=255)
                                                                                                       ggTGGTAGTTTGGGGCGGTG‑ATTTTTTCAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCGGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                   <  2:1385990/139‑1 (MQ=255)
                                                                                                       aggggTATTTTGTGGCGGTA‑AATTTTTCCAAACAAATAAAAAAACGGGAGTTGCCATGATGAGCAAAAGTAATAACCAACAGGCAGTAAATAAGTTAACAGAGATTGTCGCAACCTTTACCGCCATGATTTCTACCCGaa                                   <  2:1316883/137‑1 (MQ=255)
                                                                                                       aGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAAAGGAGGTGGCATGATGAGGGGAAGGAAAAAAAAAAAAGCAGGGAAAAAATTGAAAGAGATTGTCGCTAACTTTTCCCCCCTGATTTTTACCCCaa                                   >  2:1193768/1‑140 (MQ=255)
                                                                                                           gTATTTTGTGGCGGTG‑AATTTTCCCAGAAAAATACAAAATCTGGATTTGCCATGATGACAGAAAGTAAAAAGCAACAGGGAGTGAATAAGTTGAGAGAGTTTGCCGCTAATTTTACCGCCATGATTTCTACCCGAATGcc                               <  2:748763/140‑1 (MQ=255)
                                                                                                           gTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGAGGTGGCCTGATGAGGGAAAGTAAAAAACAAAAGGCAGGGAAAAAATTGACAGAGATTTTCGCTAACTTTACCGCCCTGATTTTTACCCCAATTcc                               >  2:1706/1‑140 (MQ=255)
                                                                                                                ttGTGGCGGTA‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                          <  1:867384/140‑1 (MQ=255)
                                                                                                                   tgGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGGGAAAGTAATAAAAAACAAGCAGTGAATAAATTGACAGAGATTGTTGCTAACTTTACCGCCATGATTTTTACCCCAATGCCTGATGACg                       >  2:1278996/1‑140 (MQ=255)
                                                                                                                     gCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                     <  1:366043/140‑1 (MQ=255)
                                                                                                                           aaaTTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGAGGAGAGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                <  2:805508/139‑1 (MQ=255)
                                                                                                                                   ccAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa        <  2:982993/140‑1 (MQ=255)
                                                                                                                                   ccAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa        <  1:1193768/140‑1 (MQ=255)
                                                                                                                                    aaaaCAAATACAAAAACTGGAGTTGCAATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa       <  2:1400740/137‑1 (MQ=255)
                                                                                                                                         aaaTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc  >  2:1295319/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                        
TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGC  >  NC_000913/3206305‑3206580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑