Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	45,474	C→A	P4P (CCC→CCA)	fixX →	putative ferredoxin FixX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,474	0	C	A	100.0%	322.5 / NA	91	P4P (CCC→CCA)	fixX	putative ferredoxin FixX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (45/46);  total (45/46)

TGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGTCTGTACAAG  >  NC_000913/45343‑45609                                                                                                                                    |                                                                                                                                        tGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAAtgt                                                                                                                                 <  1:720747/140‑1 (MQ=255)   aCCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTgg                                                                                                                               <  2:853418/140‑1 (MQ=255)             cATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAGGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACt                                                                                                                     >  2:231421/1‑140 (MQ=255)             cATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGGGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACt                                                                                                                     >  1:1176169/1‑140 (MQ=255)               ttGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTgg                                                                                                                   >  1:990451/1‑140 (MQ=255)                tGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTggg                                                                                                                  >  1:625072/1‑140 (MQ=255)                tGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTggg                                                                                                                  >  1:1118807/1‑140 (MQ=255)                tGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTggg                                                                                                                  >  1:449287/1‑140 (MQ=255)                  aTGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCg                                                                                                                >  2:1311835/1‑140 (MQ=255)                  aTGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCg                                                                                                                >  1:486578/1‑140 (MQ=255)                  aTGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCg                                                                                                                >  2:67717/1‑140 (MQ=255)                  aTGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCg                                                                                                                >  1:118305/1‑140 (MQ=255)                  aTGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCg                                                                                                                >  2:841355/1‑140 (MQ=255)                  aTGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCg                                                                                                                <  2:886392/140‑1 (MQ=255)                  aTGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCg                                                                                                                <  1:996218/140‑1 (MQ=255)                       aGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAAt                                                                                                           <  2:70105/140‑1 (MQ=255)                       aGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAAt                                                                                                           >  2:1215588/1‑140 (MQ=255)                         cgcgcCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATaa                                                                                                         >  1:740795/1‑140 (MQ=255)                         cgcgcCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATaa                                                                                                         >  2:768802/1‑140 (MQ=255)                         cgcgcCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATaa                                                                                                         >  1:108403/1‑140 (MQ=255)                           cgcCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAAt                                                                                                       >  1:1143143/1‑140 (MQ=255)                               ggAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCaa                                                                                                   <  1:274511/140‑1 (MQ=255)                                 aaCTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATg                                                                                                 <  2:118305/140‑1 (MQ=255)                                     gATGCGCAAGAAAATCCTCCGCTACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCATTAAATTCAATGTCGa                                                                                             <  2:894693/140‑1 (MQ=255)                                     gATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCATTAAATTCAATGTCGa                                                                                             <  2:723783/140‑1 (MQ=255)                                      aTGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAAt                                                                                                           <  2:130551/125‑1 (MQ=255)                                      aTGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAAt                                                                                                           >  1:130551/1‑125 (MQ=255)                                      aTGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGAt                                                                                            >  1:128124/1‑140 (MQ=255)                                        gcgcAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGa                                                                                          <  2:71888/140‑1 (MQ=255)                                          gcAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAag                                                                                        <  1:841355/140‑1 (MQ=255)                                            aaGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAagag                                                                                      <  2:666018/140‑1 (MQ=255)                                              gAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCa                                                                                    <  1:637856/140‑1 (MQ=255)                                              gAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCa                                                                                    <  2:1025306/140‑1 (MQ=255)                                               aaaaTCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCAt                                                                                   <  1:840117/140‑1 (MQ=255)                                                 aaTCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATcc                                                                                 <  1:1311835/140‑1 (MQ=255)                                                  aTCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATTTCGATGAAGAGCATCCg                                                                                <  2:444749/140‑1 (MQ=255)                                                  aTCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCg                                                                                <  2:238267/140‑1 (MQ=255)                                                    cctccGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGca                                                                              <  1:1498501/140‑1 (MQ=255)                                                      tccGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGcaca                                                                            <  1:768802/140‑1 (MQ=255)                                                      tccGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGcaca                                                                            >  1:741822/1‑140 (MQ=255)                                                      tccGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGcaca                                                                            <  2:1143143/140‑1 (MQ=255)                                                        cGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACAtt                                                                          >  1:112749/1‑140 (MQ=255)                                                        cGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACAtt                                                                          >  1:534864/1‑140 (MQ=255)                                                             cGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATtgttgt                                                                     >  1:1157009/1‑140 (MQ=255)                                                                cAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGaa                                                                  <  2:1118807/140‑1 (MQ=255)                                                                   gAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGc                                                               <  2:974886/140‑1 (MQ=255)                                                                      aGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGa                                                            <  2:486578/140‑1 (MQ=255)                                                                      aGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGa                                                            <  1:67717/140‑1 (MQ=255)                                                                        tGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATg                                                          >  1:871690/1‑140 (MQ=255)                                                                        tGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATg                                                          >  2:1150826/1‑140 (MQ=255)                                                                          ggCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCt                                                        >  1:83231/1‑140 (MQ=255)                                                                          ggCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCt                                                        >  2:1542242/1‑140 (MQ=255)                                                                             ttCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGAt                                                     >  2:1220854/1‑140 (MQ=255)                                                                               catcaATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGTCGATGCTGATaa                                                   >  1:1435542/1‑140 (MQ=255)                                                                                    aTCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAgg                                              >  2:12203/1‑140 (MQ=255)                                                                                     tCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGgc                                             >  2:784814/1‑140 (MQ=255)                                                                                       tAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGgcgc                                           <  2:990451/140‑1 (MQ=255)                                                                                         aTCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTg                                         >  2:514388/1‑140 (MQ=255)                                                                                         aTCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGACAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTg                                         >  2:1335214/1‑140 (MQ=255)                                                                                          tCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTgg                                        <  2:83231/140‑1 (MQ=255)                                                                                            aaGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAg                                      <  2:741822/140‑1 (MQ=255)                                                                                            aaGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAg                                      <  1:1220854/140‑1 (MQ=255)                                                                                            aaGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAg                                      <  2:689238/140‑1 (MQ=255)                                                                                              ggATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAgct                                    >  1:539184/1‑140 (MQ=255)                                                                                               gATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAgctg                                   <  1:200377/140‑1 (MQ=255)                                                                                               gATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAgctg                                   <  2:625072/140‑1 (MQ=255)                                                                                                aTGGCATGAAGGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAgctgc                                  <  1:231421/140‑1 (MQ=255)                                                                                                aTGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAgctgc                                  <  2:449287/140‑1 (MQ=255)                                                                                                   gCATGAAAGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAgctgcttg                               >  1:692307/1‑138 (MQ=255)                                                                                                        aaaGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGaaa                          >  2:103480/1‑140 (MQ=255)                                                                                                        aaaGGAGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGaaa                          >  2:91782/1‑140 (MQ=255)                                                                                                             aGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTg                     >  1:728410/1‑140 (MQ=255)                                                                                                             aGTGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTg                     >  2:520856/1‑140 (MQ=255)                                                                                                             aGTGACCGTTTTATGACTTCACCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTg                     >  1:49205/1‑140 (MQ=255)                                                                                                               tGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTg                     >  1:1074304/1‑138 (MQ=255)                                                                                                               tGACCGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTg                     <  2:1074304/138‑1 (MQ=255)                                                                                                                  ccGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCg                <  1:1215588/140‑1 (MQ=255)                                                                                                                  ccGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCg                <  1:91782/140‑1 (MQ=255)                                                                                                                  ccGTTTTATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCg                <  2:1157009/140‑1 (MQ=255)                                                                                                                     ttttATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAg             >  1:397345/1‑140 (MQ=255)                                                                                                                      tttATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAgg            >  2:1306641/1‑140 (MQ=255)                                                                                                                      tttATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGCCGATGAAGAGCATCCGCACATTGTTGTGACGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAgg            <  2:1345851/140‑1 (MQ=255)                                                                                                                       ttATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGTCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGt           <  2:1435542/140‑1 (MQ=255)                                                                                                                       ttATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAg             >  2:1277327/1‑138 (MQ=255)                                                                                                                       ttATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAg             <  1:1277327/138‑1 (MQ=255)                                                                                                                       ttATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGt           <  2:871690/140‑1 (MQ=255)                                                                                                                       ttATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGt           <  1:1181553/140‑1 (MQ=255)                                                                                                                       ttATGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGt           <  1:818735/140‑1 (MQ=255)                                                                                                                         aTGACTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGTCt         <  2:740795/140‑1 (MQ=255)                                                                                                                            aCTTCTCCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGTCTGTa      <  2:692307/140‑1 (MQ=255)                                                                                                                               tctcCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGTCTGTACaa   >  2:702231/1‑140 (MQ=255)                                                                                                                                ctcCAGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCATTGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGTCTGTACaag  >  1:599121/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        TGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAGGCCGATGCTGATAAACAGGCGCTGGAGCTGCTGGTGAAAGCGTGCCCCGCAGGTCTGTACAAG  >  NC_000913/45343‑45609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑