Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,221,263	C→A	G243W (GGG→TGG)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,221,263	0	C	A	100.0%	269.2 / NA	77	G243W (GGG→TGG)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/52);  total (25/52)

GTGATCGGAAACGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACC  >  NC_000913/2221126‑2221384                                                                                                                                          |                                                                                                                          gTGATCGGAAACGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACg                                                                                                                         >  1:1047063/1‑140 (MQ=255) gTGATCGGAAACGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACg                                                                                                                         <  1:774471/140‑1 (MQ=255)   gATCGGAAACGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGTGCCGCTGCCTGCGTCCAGCCACGCt                                                                                                                       <  1:198742/140‑1 (MQ=255)    aTCGGAAACGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCtt                                                                                                                      >  2:683201/1‑140 (MQ=255)        gAAACGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTa                                                                                                                  <  1:844523/140‑1 (MQ=255)          aaCGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcg                                                                                                                >  2:244958/1‑140 (MQ=255)           aCGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcgg                                                                                                               <  1:389330/140‑1 (MQ=255)           aCGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcgg                                                                                                               <  2:1047063/140‑1 (MQ=255)           aCGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcgg                                                                                                               <  2:344911/140‑1 (MQ=255)            cGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggc                                                                                                              <  2:405139/140‑1 (MQ=255)            cGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggc                                                                                                              >  2:166694/1‑140 (MQ=255)            cGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggc                                                                                                              <  2:339767/140‑1 (MQ=255)             ggTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggcg                                                                                                             <  1:245664/140‑1 (MQ=255)             ggTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggcg                                                                                                             <  2:242087/140‑1 (MQ=255)             ggTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggcg                                                                                                             <  1:166694/140‑1 (MQ=255)              gTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggcgg                                                                                                            >  2:426854/1‑140 (MQ=255)              gTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggcgg                                                                                                            >  2:673168/1‑140 (MQ=255)              gTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggc                                                                                                              >  1:347575/1‑138 (MQ=255)              gTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTAcggc                                                                                                              <  2:347575/138‑1 (MQ=255)                   gCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCatat                                                                                                       <  1:1537371/140‑1 (MQ=255)                   gCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCatat                                                                                                       <  1:539661/140‑1 (MQ=255)                    ccTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCAtata                                                                                                      >  2:568151/1‑140 (MQ=255)                    ccTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCAtata                                                                                                      <  2:975292/140‑1 (MQ=255)                    ccTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCAtata                                                                                                      <  1:45428/140‑1 (MQ=255)                       ttAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATc                                                                                                   >  2:352309/1‑140 (MQ=255)                        tAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCa                                                                                                  <  1:683201/140‑1 (MQ=255)                           aGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTa                                                                                               >  2:1009520/1‑140 (MQ=255)                              cccACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAAc                                                                                            <  1:1221535/140‑1 (MQ=255)                               ccACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACa                                                                                           >  2:1305696/1‑140 (MQ=255)                               ccACTGGTCGCACAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACa                                                                                           >  1:413577/1‑140 (MQ=255)                                cACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGTGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAg                                                                                          <  2:1274446/140‑1 (MQ=255)                                cACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAg                                                                                          <  2:1073498/140‑1 (MQ=255)                                 aCTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAgg                                                                                         >  1:1469812/1‑140 (MQ=255)                                 aCTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAgg                                                                                         >  2:271719/1‑140 (MQ=255)                                     gTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCTACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCt                                                                                     <  1:510265/140‑1 (MQ=255)                                     gTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCt                                                                                     >  1:258206/1‑140 (MQ=255)                                      tCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCta                                                                                    <  1:568151/140‑2 (MQ=255)                                      tCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTg                                                                                    <  2:285158/140‑1 (MQ=255)                                      tCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTGCAGCGAGTTCAGCTCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAACCAGGCGCTg                                                                                    <  2:305794/140‑1 (MQ=255)                                       cgcgCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGc                                                                                   >  1:1268033/1‑140 (MQ=255)                                        gcgcAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCg                                                                                  <  1:1434027/140‑1 (MQ=255)                                          gcAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGa                                                                                <  2:460770/140‑1 (MQ=255)                                          gcAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGa                                                                                <  1:922405/140‑1 (MQ=255)                                          gcAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGa                                                                                <  2:705419/140‑1 (MQ=255)                                          gcAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGa                                                                                <  1:164312/140‑1 (MQ=255)                                           cAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGAc                                                                               <  1:271719/140‑1 (MQ=255)                                            aGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACt                                                                              <  1:1009520/140‑1 (MQ=255)                                              aaCATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCGGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCa                                                                            <  1:710646/140‑1 (MQ=255)                                                    tttCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCTGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGa                                                                      <  2:696735/140‑1 (MQ=255)                                                    tttCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGa                                                                      <  1:590287/140‑1 (MQ=255)                                                      tCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACg                                                                    >  2:1234520/1‑140 (MQ=255)                                                      tCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACg                                                                    <  1:979004/140‑1 (MQ=255)                                                        agcagcCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGgt                                                                  >  2:99497/1‑140 (MQ=255)                                                         gcagcCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGgtg                                                                 <  2:1536020/140‑1 (MQ=255)                                                         gcagcCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGgtg                                                                 <  2:413577/140‑1 (MQ=255)                                                          cagcCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGgtgt                                                                >  2:35903/1‑140 (MQ=255)                                                           agcCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGtgtt                                                               >  2:136129/1‑140 (MQ=255)                                                           agcCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGtgtt                                                               <  1:426854/140‑1 (MQ=255)                                                              cAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTa                                                            <  2:617604/140‑1 (MQ=255)                                                              cAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTa                                                            <  1:61000/140‑1 (MQ=255)                                                              cAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTa                                                            <  1:1305696/140‑1 (MQ=255)                                                                     cGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTa                                                     <  1:474385/140‑1 (MQ=255)                                                                     cGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTa                                                     <  1:35903/140‑1 (MQ=255)                                                                       gAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTAcc                                                   <  2:1268033/140‑1 (MQ=255)                                                                           tggctggcGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCt                                               >  1:625571/1‑140 (MQ=255)                                                                                    tGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACcgcg                                      <  1:1539631/140‑1 (MQ=255)                                                                                       cGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCg                                   >  2:655719/1‑140 (MQ=255)                                                                                                 gTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCaa                         <  2:1179587/140‑1 (MQ=255)                                                                                                            ccaTCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACg              <  1:1479495/140‑1 (MQ=255)                                                                                                            ccaTCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACg              >  2:80064/1‑140 (MQ=255)                                                                                                            ccaTCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACg              <  1:99497/140‑1 (MQ=255)                                                                                                             caTCACCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGc             <  1:136129/140‑1 (MQ=255)                                                                                                                 aCCGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGt         <  1:1234520/140‑1 (MQ=255)                                                                                                                  ccGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACg              >  2:1151829/1‑134 (MQ=255)                                                                                                                  ccGCGCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACg              <  1:1151829/134‑1 (MQ=255)                                                                                                                   cgcgCCGCTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGtca       >  2:405796/1‑140 (MQ=255)                                                                                                                     cgccgcTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGtcatc     <  2:1469812/140‑1 (MQ=255)                                                                                                                        cgcTGCCTGCGTCCAGCCACGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCAcc  <  2:625571/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                          GTGATCGGAAACGGTGATGCCTTTAAAGCCCCACTGGTCGCGCAGAACATCTTTCAGCAGCCAGGAATCGGAGGTGGCTGGCGTGCCGTTCAGCGAGTTCAGCGCCACCATCACCGCGCCGCTGCCTGCGTCCAGCCCCGCTTTGTACGGCGGCATATAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACC  >  NC_000913/2221126‑2221384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑