Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,640,196	C→A	P29H (CCC→CAC)	uspA →	universal stress global stress response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,640,196	0	C	A	100.0%	279.8 / NA	79	P29H (CCC→CAC)	uspA	universal stress global stress response regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/49);  total (30/49)

AAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTGACCGAGCTTTCC  >  NC_000913/3640060‑3640332                                                                                                                                         |                                                                                                                                         aaGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTa                                                                                                                                       <  1:918498/140‑1 (MQ=255)     aaCGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAAt                                                                                                                                   <  1:877324/140‑1 (MQ=255)      aCGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATg                                                                                                                                  >  1:1274434/1‑140 (MQ=255)            gCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAg                                                                                                                            <  2:1313608/140‑1 (MQ=255)                 ccAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTtct                                                                                                                       <  2:1013642/140‑1 (MQ=255)                 ccAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTtct                                                                                                                       <  1:1543224/140‑1 (MQ=255)                  cAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTtctc                                                                                                                      >  2:366399/1‑140 (MQ=255)                      cATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGAt                                                                                                                  <  1:1076706/140‑1 (MQ=255)                       aTCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATc                                                                                                                 <  1:1374472/140‑1 (MQ=255)                           aCAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACg                                                                                                             <  1:624126/140‑1 (MQ=255)                           aCAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACg                                                                                                             <  2:1250567/140‑1 (MQ=255)                           aCAACTTTATGGAAGAAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACg                                                                                                             <  2:730787/140‑1 (MQ=255)                                     ggAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTaaa                                                                                                   <  1:334773/140‑1 (MQ=255)                                     ggAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTaaa                                                                                                   <  2:251635/140‑1 (MQ=255)                                         ggAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAactac                                                                                               >  2:989141/1‑140 (MQ=255)                                          gAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAactact                                                                                              <  1:458505/140‑1 (MQ=255)                                          gAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAactact                                                                                              <  2:1274434/140‑1 (MQ=255)                                            gTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGGTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTActct                                                                                            <  1:1388775/140‑1 (MQ=255)                                            gTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTActct                                                                                            <  2:495736/140‑1 (MQ=255)                                            gTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTActct                                                                                            <  2:753954/140‑1 (MQ=255)                                            gTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTActct                                                                                            <  1:223021/140‑1 (MQ=255)                                            gTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTActct                                                                                            <  1:885297/140‑1 (MQ=255)                                            gTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTActct                                                                                            <  1:1221543/140‑1 (MQ=255)                                             tAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTg                                                                                           <  2:1004134/140‑1 (MQ=255)                                                  cTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCta                                                                                      <  1:1416871/140‑1 (MQ=255)                                                     tGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATac                                                                                   >  2:930460/1‑140 (MQ=255)                                                     tGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATac                                                                                   <  1:1151364/140‑1 (MQ=255)                                                     tGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATac                                                                                   >  2:1329433/1‑140 (MQ=255)                                                     tGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATac                                                                                   >  2:1383459/1‑140 (MQ=255)                                                     tGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATac                                                                                   >  2:730822/1‑140 (MQ=255)                                                     tGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATac                                                                                   >  1:805585/1‑140 (MQ=255)                                                     tGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATac                                                                                   >  1:1315523/1‑140 (MQ=255)                                                     tGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATac                                                                                   >  1:308250/1‑140 (MQ=255)                                                     tGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATac                                                                                   >  1:389866/1‑140 (MQ=255)                                                          tataAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCgg                                                                              >  1:881603/1‑140 (MQ=255)                                                           ataAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCggg                                                                             >  1:791012/1‑140 (MQ=255)                                                           ataAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACATAGATGTAAACTACTCTGACCTATACACCggg                                                                             >  1:1381581/1‑140 (MQ=255)                                                             aaaCACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCt                                                                           >  1:352424/1‑140 (MQ=255)                                                               acacATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTa                                                                         <  1:730822/140‑1 (MQ=255)                                                                cacaTTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTAt                                                                        <  1:989141/140‑1 (MQ=255)                                                                 acaTTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTAtt                                                                       >  2:1368177/1‑140 (MQ=255)                                                                    ttCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGAt                                                                    <  1:1163933/140‑1 (MQ=255)                                                                      ctcATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGAtgt                                                                  <  1:985169/140‑1 (MQ=255)                                                                       tcATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGAtgtg                                                                 <  1:1415475/140‑1 (MQ=255)                                                                         aTCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGaa                                                               >  1:101364/1‑140 (MQ=255)                                                                          tCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAAt                                                              <  2:1322615/140‑1 (MQ=255)                                                                          tCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAAt                                                              <  2:269139/140‑1 (MQ=255)                                                                             cgGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTg                                                           >  1:153857/1‑140 (MQ=255)                                                                               gTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTggg                                                         >  1:537977/1‑140 (MQ=255)                                                                               gTCGACCTCTCCCCGGAAAGCAAAGTTCTGG‑‑GAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTg                                                       >  1:484316/1‑140 (MQ=255)                                                                                 cGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATCCACCGGGCTTATTGATGTGAATCTGGGTg                                                       <  2:153857/140‑1 (MQ=255)                                                                                 cGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTg                                                       <  1:930460/140‑1 (MQ=255)                                                                                 cGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTg                                                       <  1:717497/140‑1 (MQ=255)                                                                                   aCCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGat                                                     >  2:294471/1‑140 (MQ=255)                                                                                   aCCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGat                                                     >  2:1266155/1‑140 (MQ=255)                                                                                    ccTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGata                                                    <  2:881603/140‑1 (MQ=255)                                                                                          cccGGAAAGCTAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGa                                              >  2:1295593/1‑140 (MQ=255)                                                                                          cccGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGa                                              >  2:285355/1‑140 (MQ=255)                                                                                            cGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGaaa                                            <  2:1485037/140‑1 (MQ=255)                                                                                              gAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACg                                          <  2:411527/140‑1 (MQ=255)                                                                                                  gCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCAtc                                      <  1:420651/140‑1 (MQ=255)                                                                                                  gCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCAtc                                      <  1:1297050/140‑1 (MQ=255)                                                                                                             gTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGac                           <  1:1266155/140‑1 (MQ=255)                                                                                                             gTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGac                           <  2:805585/140‑1 (MQ=255)                                                                                                             gTAGAGAAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGac                           <  1:1232045/140‑1 (MQ=255)                                                                                                                 agaAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACAcat                       <  1:294471/140‑1 (MQ=255)                                                                                                                  gaAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACAcatc                      >  1:5996/1‑140 (MQ=255)                                                                                                                  gaAAGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACAcatc                      >  1:207639/1‑140 (MQ=255)                                                                                                                   aaaGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGac                           >  2:284639/1‑134 (MQ=255)                                                                                                                   aaaGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGac                           <  1:284639/134‑1 (MQ=255)                                                                                                                   aaaGCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACAcatca                     <  2:352424/140‑1 (MQ=255)                                                                                                                      gCAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGc                  <  1:366399/140‑1 (MQ=255)                                                                                                                       cAGTCTCTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCa                 <  1:285355/140‑1 (MQ=255)                                                                                                                          tctcTATGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTg              >  1:1540275/1‑140 (MQ=255)                                                                                                                                tGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTGACCGAg        <  2:484316/140‑1 (MQ=255)                                                                                                                                tGGCTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTGACCGAg        <  2:1540275/140‑1 (MQ=255)                                                                                                                                   cTCGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTGACCGAGCtt     <  1:535042/140‑1 (MQ=255)                                                                                                                                     cGCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTGACCGAGCTTTc   >  2:773799/1‑140 (MQ=255)                                                                                                                                      gCCACTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGTTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTGACCGAGCTTTcc  >  1:992183/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         AAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGACCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGCTCGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTGACCGAGCTTTCC  >  NC_000913/3640060‑3640332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑