Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	553,213	G→T	intergenic (‑113/+5)	purE ← / ← lpxH	N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	553,213	0	G	T	100.0%	306.1 / NA	89	intergenic (‑113/+5)	purE/lpxH	N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/42);  total (47/42)

CCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCG  >  NC_000913/553079‑553349                                                                                                                                       |                                                                                                                                         ccGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGtttttt                                                                                                                                     <  2:713114/140‑1 (MQ=255) ccGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGtttttt                                                                                                                                     >  2:1349807/1‑140 (MQ=255)       tattGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAAc                                                                                                                               >  2:746382/1‑140 (MQ=255)          tGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGa                                                                                                                            >  2:719848/1‑140 (MQ=255)              ggAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGTGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAAt                                                                                                                        >  1:666730/1‑140 (MQ=255)              ggAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAAt                                                                                                                        >  2:1491340/1‑140 (MQ=255)                  gACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAAt                                                                                                                    <  1:719848/140‑1 (MQ=255)                  gACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAAt                                                                                                                    <  2:1485915/140‑1 (MQ=255)                   aCATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATc                                                                                                                   >  1:799849/1‑140 (MQ=255)                      tGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGc                                                                                                                <  2:188592/140‑1 (MQ=255)                       gCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCt                                                                                                               <  2:12431/140‑1 (MQ=255)                          tCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCaa                                                                                                            >  2:576749/1‑140 (MQ=255)                               aaaCTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCa                                                                                                       <  1:1349807/140‑1 (MQ=255)                               aaaCTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCa                                                                                                       >  2:238132/1‑140 (MQ=255)                                   tCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCt                                                                                                               >  2:542448/1‑128 (MQ=255)                                   tCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCt                                                                                                               <  1:542448/128‑1 (MQ=255)                                     cTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATccgcc                                                                                                 >  1:961080/1‑140 (MQ=255)                                     cTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATccgcc                                                                                                 >  1:1181694/1‑140 (MQ=255)                                     cTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATccgcc                                                                                                 >  2:1187640/1‑140 (MQ=255)                                      tgtgGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGTGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATccgccg                                                                                                <  1:1305434/140‑1 (MQ=255)                                          gtgCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGAc                                                                                            >  2:564665/1‑140 (MQ=255)                                             cacaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACttt                                                                                         <  1:576749/140‑1 (MQ=255)                                             cacaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACttt                                                                                         >  2:471565/1‑140 (MQ=255)                                             cacaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACttt                                                                                         <  2:385042/140‑1 (MQ=255)                                             cacaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACttt                                                                                         <  1:1031446/140‑1 (MQ=255)                                             cacaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACttt                                                                                         >  1:385042/1‑140 (MQ=255)                                             cacaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATAAATCAGCTCAACGTCATCCGCCGTGACttt                                                                                         <  2:819601/140‑1 (MQ=255)                                                   tctcGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCAt                                                                                   <  1:471565/140‑1 (MQ=255)                                                     tcGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTg                                                                                 >  2:1323933/1‑140 (MQ=255)                                                     tcGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTg                                                                                 >  1:904272/1‑140 (MQ=255)                                                     tcGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTg                                                                                 >  1:162702/1‑140 (MQ=255)                                                        gCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAAc                                                                              <  1:376000/140‑1 (MQ=255)                                                         cTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAAcc                                                                             <  1:551813/140‑1 (MQ=255)                                                         cTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAAcc                                                                             <  2:236204/140‑1 (MQ=255)                                                          tttAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCt                                                                            >  2:1330241/1‑140 (MQ=255)                                                                 ggCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTAt                                                                     >  1:1233388/1‑140 (MQ=255)                                                                   cacaGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGc                                                                   <  2:1214744/140‑1 (MQ=255)                                                                   cacaGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGc                                                                   >  1:1214744/1‑140 (MQ=255)                                                                     caGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCa                                                                 >  1:20110/1‑140 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGTGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  2:666730/140‑1 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGGGGGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  2:432839/140‑1 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  2:1181694/140‑1 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                >  2:1387617/1‑140 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  1:1293447/140‑1 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  1:1517567/140‑1 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  2:1233388/140‑1 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  1:610501/140‑1 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGATTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  1:1491340/140‑1 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCGGTGAAATCAGAAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  2:1403782/140‑1 (MQ=255)                                                                      agagAATAGCACGGAAAGAGAGCAAGAAAACCGTTTGCGTGGCTGGAAAATGAGCAAAGTTAGGGTTTTTTAAAAACGGAAAATAAATCAGCTCAACTTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  2:904272/140‑1 (MQ=255)                                                                       gagaATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAgg                                                               <  1:1116197/140‑1 (MQ=255)                                                                       gagaATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAgg                                                               <  1:1323933/140‑1 (MQ=255)                                                                       gagaATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAgg                                                               <  1:561638/140‑1 (MQ=255)                                                                           aTAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCAcc                                                           >  1:1266509/1‑140 (MQ=255)                                                                             aGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCa                                                         >  2:704733/1‑140 (MQ=255)                                                                             aGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCa                                                         >  2:996493/1‑140 (MQ=255)                                                                             aGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCa                                                         <  1:1387617/140‑1 (MQ=255)                                                                             aGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCa                                                         >  1:1274081/1‑140 (MQ=255)                                                                               cACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGt                                                       >  1:408460/1‑140 (MQ=255)                                                                               cACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGt                                                       >  1:1153957/1‑140 (MQ=255)                                                                                  ggAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACTCAGTAcc                                                    >  2:812710/1‑140 (MQ=255)                                                                                  ggAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTAcc                                                    >  1:1301332/1‑140 (MQ=255)                                                                                  ggAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTAcc                                                    >  1:6806/1‑140 (MQ=255)                                                                                   gAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCa                                                   >  1:104368/1‑140 (MQ=255)                                                                                    aaaGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                >  1:741232/1‑126 (MQ=255)                                                                                    aaaGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                                <  2:741232/126‑1 (MQ=255)                                                                                    aaaGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCAc                                                  >  2:1404158/1‑140 (MQ=255)                                                                                    aaaGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCAc                                                  >  2:656370/1‑140 (MQ=255)                                                                                      agagagCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCAcgc                                                <  1:846608/140‑1 (MQ=255)                                                                                         gagCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGaa                                             >  2:1087476/1‑140 (MQ=255)                                                                                            cAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAg                                          >  1:1203870/1‑140 (MQ=255)                                                                                             aaGGAAAACGGTTGGGTGGTTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGc                                         <  1:376096/140‑1 (MQ=255)                                                                                             aaGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGc                                         <  1:656370/140‑1 (MQ=255)                                                                                              aGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCa                                        >  1:81780/1‑140 (MQ=255)                                                                                              aGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCa                                        >  2:98023/1‑140 (MQ=255)                                                                                               ggAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAg                                       >  2:310736/1‑140 (MQ=255)                                                                                               ggAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAg                                       <  1:310736/140‑1 (MQ=255)                                                                                                   aaCGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTg                                   >  1:1565846/1‑140 (MQ=255)                                                                                                    aCGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGc                                  >  1:659231/1‑140 (MQ=255)                                                                                                      ggTTGCGTGGCTGTGAAGTCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTg                                >  1:531866/1‑140 (MQ=255)                                                                                                        ttGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGAt                              <  1:564665/140‑1 (MQ=255)                                                                                                        ttGCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGAt                              <  1:746382/140‑1 (MQ=255)                                                                                                          gCGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTg                            >  1:432908/1‑140 (MQ=255)                                                                                                           cGTGGCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTgg                           >  2:909033/1‑140 (MQ=255)                                                                                                              ggCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCACCGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGa                        <  2:1301332/140‑1 (MQ=255)                                                                                                              ggCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGa                        <  2:6806/140‑1 (MQ=255)                                                                                                              ggCTGTGAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGa                        <  2:1266509/140‑1 (MQ=255)                                                                                                                  gtgAAATCAGCAAAGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAg                    >  2:697216/1‑140 (MQ=255)                                                                                                                             aaaGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAccg         >  2:349089/1‑140 (MQ=255)                                                                                                                              aaGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAccgc        <  1:98023/140‑1 (MQ=255)                                                                                                                               aGTTGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAccgcc       <  2:531866/140‑1 (MQ=255)                                                                                                                                    cGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCg  <  2:1203870/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCG  >  NC_000913/553079‑553349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑