Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,628,6250AC22.6% 202.9 / 30.3 84V105G (GTC→GGC) pdeFcyclic di‑GMP phosphodiesterase PdeF
Reads supporting (aligned to +/- strand):  ref base A (58/7);  new base C (0/19);  total (58/26)
Fisher's exact test for biased strand distribution p-value = 1.99e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 101 total aligned reads displayed.

GCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGA  >  NC_000913/2628486‑2628760
                                                                                                                                            |                                                                                                                                       
gCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGa                                                                                                                                         <  2:1262204/140‑1 (MQ=255)
 cTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAc                                                                                                                                        >  1:2242658/1‑140 (MQ=255)
 cTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAc                                                                                                                                        >  1:20595/1‑140 (MQ=255)
  tCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAcc                                                                                                                                       <  2:297748/140‑1 (MQ=255)
   cGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAccc                                                                                                                                      <  2:742435/140‑1 (MQ=255)
       aatAATCCTAAAAAGGCAGAAAACTGGAAAATTATCAAAAACAGCGTTGCAGGAAACACAATCTCCAAAAATATCCGCTGGGAAATAAAGC‑GGTTACCCCCTGGTAAACTGTTTTTCCGCGGGGGAGAAAACCCCCttaa                                                                                                                                  <  2:2435438/138‑4 (MQ=255)
         tAATCCTAAAAATGCAGCAAACGGAAAAAGTATCAAAAACAGCGTTGCAGAAAACACAATCTGCCAGAATATACGCTGGAAAATAAAAC‑GGGTATCCCCATGTAAAACGTTGTTCCGCGGGGGAGAAAAGCCCCTGTAAc                                                                                                                                <  2:271703/140‑1 (MQ=255)
              cTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGccc                                                                                                                           >  2:147082/1‑140 (MQ=255)
              cTACAAATGCAGCAAACGGAAAAAGTATAAGAAACAGCGTTGCAGAAAACACAATCTGCCAGAATATACGCGGGAAAATAAAAC‑GGGTACCACCATGTGAACCGTTGTTCCGCGGGGGAGAAAAGCCCCTGTAACCGccc                                                                                                                           <  2:843579/140‑1 (MQ=255)
               tACAAATGCAGCAAACTGAAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCCCCATGTGAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAACCGcccc                                                                                                                          <  1:147082/140‑1 (MQ=255)
                aCAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCa                                                                                                                         >  1:580277/1‑140 (MQ=255)
                   aaggcagaaAACTGAAAAAGTAAAAAAAACGGGGTGGCAGAAAACACAATCGGCCAGAATATACGC‑GGGAAAATAAACGGGGTACCACCTTGTAAACCGTTTTTCCGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACa                                                                                                                      <  2:2421719/137‑1 (MQ=255)
                   aaTGCAGCAAACTGAAAAAGTATCAGAAACAGCGTTGCAGAAAACAAAATCTGCAAGAATATACGCGGGAAAATAAAAC‑GGGTATCACCATGTGAAACGTTGTTCCGCGGGGGAGTAAAGCCCCTGTAACCGCCCCAACa                                                                                                                      <  2:138308/140‑1 (MQ=255)
                      gcagaaAACGGAAAAAGTATCAAAAACAGCTTTGCAGGAAACACAATCTGCCAGAATATACGGTGGAAAATTAACG‑GGGTATCACCATGTAAAGCTTTGTTACGCCGGGGAGTAAAGCCCTTGTAACCGCCCCAACagag                                                                                                                   <  2:1655377/140‑1 (MQ=255)
                       cagaaAACTGGAAAAGTATCAGAAACAGCGTTGCAGAAAACACAATCTGCAAGAATATACGCGGGGAAATAAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACAgaga                                                                                                                  <  2:2314471/140‑1 (MQ=255)
                       aaaaaaaCTGAAAAAGTATAAAAAACAGCGTTGCAGGAAACAAATTCTGCAAGAATATCCGCGGGGAAATTAAAC‑GGGTATCCCCATGTAAAACTTTGTTACGCCGGGGAGTAAAGCCCCTTTAACCGCCCCAACAgaga                                                                                                                  <  2:1509258/136‑1 (MQ=255)
                        agcaAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaa                                                                                                                 >  2:895973/1‑140 (MQ=255)
                         gcaAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaat                                                                                                                >  2:382885/1‑140 (MQ=255)
                         gcaAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaat                                                                                                                >  2:147838/1‑140 (MQ=255)
                          caAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaatg                                                                                                               >  1:1571349/1‑140 (MQ=255)
                          caAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaatg                                                                                                               >  1:1734471/1‑140 (MQ=255)
                          caAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaatg                                                                                                               >  1:2255/1‑140 (MQ=255)
                                 gAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaatgataatga                                                                                                        >  2:1074041/1‑140 (MQ=255)
                                 gAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGCATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaatgataatga                                                                                                        >  1:2354807/1‑140 (MQ=255)
                                   aaaGTATAAGAAACAGCGTTGAAGAAAACACAATCTGCCAAAATATCCGCGGGGAAATAAACC‑GGGTATCCCCATGTAAACGGTTGTTCCGCGGGGGAGAAAAGCCCCTTAAACCGCCCCAACAGAGAatgataatgata                                                                                                      <  2:1203582/140‑1 (MQ=255)
                                     actATCAAAAAGTGCGTCGCAGAAACCCCAATCTCCAAAAATAACCGCGGGGAAAAAAAGC‑GGGTATCACTATGTAAATTTTTTTTACGCGGGGAATTAAAGACCCTGTAACCGCCCCAACAGAGAATgataatgataat                                                                                                    <  2:580277/138‑1 (MQ=255)
                                      gTATCAGAAACAGCGTTGCAGAAAACACAATCTGCAAGAATATACGCGGGAAAATAAAAC‑GGGTATCCCCATGTAAAACTTTGTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGataata                                                                                                   <  2:65800/140‑1 (MQ=255)
                                        aTCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATaaa                                                                                                 >  2:2267110/1‑140 (MQ=255)
                                         tCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATaaaa                                                                                                >  1:1720523/1‑140 (MQ=255)
                                           aGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAAtt                                                                                              >  1:965344/1‑140 (MQ=255)
                                           aGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAAtt                                                                                              >  1:2397025/1‑140 (MQ=255)
                                           aGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGACCCAACAGAGAATGATAATGGTAATAAAAtt                                                                                              >  2:2116654/1‑140 (MQ=255)
                                               aCAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGcc                                                                                          <  1:147838/140‑1 (MQ=255)
                                                cAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCg                                                                                         >  1:2326017/1‑140 (MQ=255)
                                                cAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCg                                                                                         >  1:1334306/1‑140 (MQ=255)
                                                cAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCg                                                                                         >  1:610945/1‑140 (MQ=255)
                                                   cGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTa                                                                                      >  2:966224/1‑140 (MQ=255)
                                                   cGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTa                                                                                      >  1:1020140/1‑140 (MQ=255)
                                                   cGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTa                                                                                      >  1:1363262/1‑140 (MQ=255)
                                                        cAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGAt                                                                                 >  1:1589815/1‑140 (MQ=255)
                                                               cacaATCTGCAAGAATATACGCTGGAAAATAAACC‑GGGTACCACCATGTAAAACGTTTTTACGCGGGGGAAAAAAGCCCCTTTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGttt                                                                          <  2:2255/140‑1 (MQ=255)
                                                               caaaaTCTGCAAAAATATCCGCTGGAAAATAAACG‑GGGTATCCCCTTGTAAAACTTTTTTCCGCCGGGGAAAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGttt                                                                          <  2:1734471/137‑1 (MQ=255)
                                                               aaaaaccTGCAAGAAAATCCGCGGGGAAATAAAAC‑GGGAATCCCCTTGTAAAAGGTTTTTCCGCGGGGGAGAAAAGCCCCTTTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGttt                                                                          <  2:2354807/134‑1 (MQ=255)
                                                                acaATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTc                                                                         >  1:1468061/1‑140 (MQ=255)
                                                                  aaTCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGt                                                                       >  2:203573/1‑140 (MQ=255)
                                                                  aaTCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGt                                                                       >  1:1725642/1‑140 (MQ=255)
                                                                  aaTCTGCAAGAATATACGCTGGAAAATAAAAG‑GGGTATCACCATGTAAAACGTTGTTCCGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGt                                                                       <  2:2242658/140‑1 (MQ=255)
                                                                   aTCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTa                                                                      >  1:1556934/1‑140 (MQ=255)
                                                                   aTCTGCAAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGAAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCNTTNNGTa                                                                      >  2:2380119/1‑140 (MQ=255)
                                                                     cTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaaa                                                                    >  1:2407203/1‑140 (MQ=255)
                                                                     cTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaaa                                                                    >  1:1436674/1‑140 (MQ=255)
                                                                      tGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaaaa                                                                   >  2:2063335/1‑140 (MQ=255)
                                                                         cAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAcc                                                                >  1:452725/1‑140 (MQ=255)
                                                                         cAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAcc                                                                >  1:581823/1‑140 (MQ=255)
                                                                         cAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAcc                                                                >  1:934835/1‑140 (MQ=255)
                                                                            aaTATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCGGGGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAg                                                             <  2:581823/140‑1 (MQ=255)
                                                                            aaTATACGCAGGAAAATTAAAG‑GGGTATCACCATGAGAAATTTTTTTGCGCGGGGAAAAAAGGCCCCTGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAg                                                             <  2:104404/140‑1 (MQ=255)
                                                                              tatcCGCGGGAAAATTAAAC‑GGGTACCACCATGTGAAACGTTGTTACGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGct                                                           <  2:1468061/140‑1 (MQ=255)
                                                                              tataCGCTGGGAAATTAAAC‑GGGTATCACCATGTAAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAAAGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCAAGct                                                           <  2:275315/140‑1 (MQ=255)
                                                                              tataCGCTGGAAAATTAACC‑GGTTACCCCCATGTAAAACGTTTTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGct                                                           <  2:1882118/140‑1 (MQ=255)
                                                                                      gggAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatat                                                   >  2:1230487/1‑140 (MQ=255)
                                                                                       ggAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAtt                                                  >  1:1482025/1‑140 (MQ=255)
                                                                                        gAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAttt                                                 >  2:1979090/1‑140 (MQ=255)
                                                                                              aaaC‑GGGTACCCCCATGTGAAACGTTGTTCCGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGAc                                           <  1:1074041/140‑1 (MQ=255)
                                                                                               aaC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACa                                          >  1:1109346/1‑140 (MQ=255)
                                                                                                 c‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaa                                        >  1:98017/1‑140 (MQ=255)
                                                                                                  gggttATCCCCATGTAAAACGTTGTTCCGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaa                                        <  2:934835/136‑1 (MQ=255)
                                                                                                  ggggTATCCCCATGTAAAACGTTTTTCCGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaa                                        <  2:1725642/139‑1 (MQ=255)
                                                                                                     gTATCACCATGTAAAATGTTGTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAAt                                     <  2:2397025/140‑1 (MQ=255)
                                                                                                       aTCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATcc                                   >  2:1155405/1‑140 (MQ=255)
                                                                                                       aTCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATcc                                   >  1:1575352/1‑140 (MQ=255)
                                                                                                        tCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATccc                                  >  2:1898538/1‑140 (MQ=255)
                                                                                                        tCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATccc                                  <  1:418233/140‑1 (MQ=255)
                                                                                                        cccccATGTAAACCGTTTTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATccc                                  <  2:1589815/137‑1 (MQ=255)
                                                                                                         ccccATGTAAAACTTTGTTACGCGGGGGAGAAAAGCCCTTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                                 <  2:453889/138‑1 (MQ=255)
                                                                                                         cACCATGTGAAACTTTGTTCCGCGGGGGAAAAAAGCCCCTAAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                                 <  2:2326017/140‑1 (MQ=255)
                                                                                                         cACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                                 <  1:1979090/140‑1 (MQ=255)
                                                                                                             aTGTGAAACGTTGTTACGCCGGGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAgg                             <  1:1155405/140‑1 (MQ=255)
                                                                                                              tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGc                            >  2:2254116/1‑140 (MQ=255)
                                                                                                              tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGc                            >  2:2175072/1‑140 (MQ=255)
                                                                                                               gtgAAGCTTTGTTGCGCGGGGTGGTAAAGGCCCTGTAGCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                           <  1:173624/140‑1 (MQ=255)
                                                                                                               gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCgg                                             >  2:792209/1‑122 (MQ=255)
                                                                                                               gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCgg                                             <  1:792209/122‑1 (MQ=255)
                                                                                                               gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                           >  1:844171/1‑140 (MQ=255)
                                                                                                               gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                           >  2:1363515/1‑140 (MQ=255)
                                                                                                                  aaaCGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATc                        >  2:1823907/1‑140 (MQ=255)
                                                                                                                  aaaCGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATc                        >  2:167160/1‑140 (MQ=255)
                                                                                                                   aaCGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATcc                       >  1:1498694/1‑140 (MQ=255)
                                                                                                                        tgttACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGc                  >  1:2294360/1‑140 (MQ=255)
                                                                                                                         gttACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCa                 >  1:1247075/1‑140 (MQ=255)
                                                                                                                         gttACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCa                 >  2:2001402/1‑140 (MQ=255)
                                                                                                                             cGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATgc             >  2:694735/1‑140 (MQ=255)
                                                                                                                                gggggAAAAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATgccgc          <  2:452725/139‑1 (MQ=255)
                                                                                                                                  gggAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGccc        <  1:203573/140‑1 (MQ=255)
                                                                                                                                  gggAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGccc        >  1:77063/1‑140 (MQ=255)
                                                                                                                                  gggAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGccc        <  2:1020140/140‑1 (MQ=255)
                                                                                                                                  gggAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGccc        >  2:108923/1‑140 (MQ=255)
                                                                                                                                    gagaAAAGCCCCTTTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAg      <  2:1720523/140‑1 (MQ=255)
                                                                                                                                    gAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAg      <  2:965344/140‑1 (MQ=255)
                                                                                                                                        aaaGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGa  >  1:1439389/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                       
GCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAAC‑GGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGA  >  NC_000913/2628486‑2628760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑