Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,205	0	A	C	28.8%	133.0 / 12.8	59	S215R (AGT→AGG) ‡	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base A (42/0);  new base C (0/17);  total (42/17)
Fisher's exact test for biased strand distribution p-value = 3.60e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCG  >  NC_000913/3556068‑3556344                                                                                                                                            |                                                                                                                                            cccTCGGCACTGACGCGTTTTTCACCGACGAAAAAAAAGCGTTGGGTGATATTTTCCCTTTCGACTTC‑AAGCGAGACGGTATTCCCCGGCCCCTGGTC‑GCGCGGCAGTTTATAAATATTCTGTTCATGCAGGAAAAACCt                                                                                                                                           <  2:27132/139‑1 (MQ=255) accTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCGTGCAGGGAAAAACt                                                                                                                                           >  1:2235434/1‑140 (MQ=255)      ggCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCg                                                                                                                                      >  2:531139/1‑140 (MQ=255)       gCACTGACGCGTTTTTCCCCGACAAAAAAAAAGCGTTCGGTGATTTTTTCTCTTTCGACTTC‑AAGCGAGCCGGTATCCCCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATTTTTTTTTCATGCAGGGAAAACCCCCCCGc                                                                                                                                     <  2:477219/140‑1 (MQ=255)             aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                               >  1:719564/1‑140 (MQ=255)             aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                               >  2:2415190/1‑140 (MQ=255)             aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                               >  1:1437619/1‑140 (MQ=255)             aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGG‑CCCTGGAC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTa                                                                                                                              >  1:658623/1‑140 (MQ=255)                cgcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGc                                                                                                                            >  2:1047862/1‑140 (MQ=255)                 gttttcCACCGACGAAAAAAAGGCGTTCGGTGTTTTTTTCCCTTTCGACTTC‑AAGCGAGACGTTTTTCCCCGGCCCCTGGTC‑GCGCGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCg                                                                                                                           <  2:1495717/138‑1 (MQ=255)                  cTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGa                                                                                                                          >  1:1968689/1‑140 (MQ=255)                  cTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGa                                                                                                                          >  1:2015487/1‑140 (MQ=255)                         ccaacgaaAAAAGAGCGTGCGGTGATTTTTTCCCTTTCGATTTC‑AAGGGAGACGTTATTCCCCGGCCCCTGGTC‑GCGGGGCATTTTAAAAATTTTCTTTTCATGCAGAAAAAACCCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                   <  2:1018770/137‑1 (MQ=255)                         ccGACAAAAAAAACGCGTTCGGTGTTATTTTCATTTTCG‑CTTCAAAGCAACAATATATTCCCCGCCCCCTGGCC‑GCGCGGCAGGTTAAAAATTTTCGGTTAACACAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACg                                                                                                                   <  2:719564/140‑1 (MQ=255)                         ccGACAAAAAAAAAGCTTTGGGTAATTTTTTCACTTTCGACTTC‑AAGCGAGACGGTATTCCCCGGCCCCGGGCC‑GCGCGGCGGGTTATAAATTTTCTGTTCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACg                                                                                                                   <  2:733158/140‑1 (MQ=255)                           gaggaaaaaaagGCGTGGGGTTATTTTTTCATTTCCGCCTTC‑AAGCGGGAGGTTTTTCCCCGCCCCCTGGTC‑GCGCGGAAGGTTATAAATTTTCTGTTCAGGGAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                                 <  2:2197134/134‑1 (MQ=255)                             cgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCa                                                                                                               >  1:495372/1‑140 (MQ=255)                             cgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCa                                                                                                               >  2:80950/1‑140 (MQ=255)                                         gTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcgc                                                                                                   >  2:2414721/1‑140 (MQ=255)                                           tCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                 >  1:1029731/1‑140 (MQ=255)                                           ggggTGATTTTTTCACTTCCGGCTCC‑AGGGGAGCCGTTATCCCCGGGCCCCTGTTC‑GTACGGATGGTTACGAATTTCGTTTCCACGCGGGAAAACGCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                 <  2:1672593/138‑1 (MQ=255)                                           gcGGTGATTTTTTCTCTTCCGACTTC‑AGGCGAG‑TGGATTTACCCGGCCCCCGGGCGGCGCGGCAGTTTATAAATTTTTTGTTCAGGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                 <  2:1365276/139‑1 (MQ=255)                                            gggTTATTTTTTCCTTTTCGACTTA‑AAGCGAGGGGTTATTCCCCGGCCCCTGGTC‑GCGGGGAGGGTTATGAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc                                                                                                <  2:1673255/139‑1 (MQ=255)                                             ggTGATTTTTTCTCTTTCGACTTA‑AAGCGAGACGGTATTCCCCGCCCCGTGGCC‑GCGCGGCAGTTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca                                                                                               <  2:1968689/140‑1 (MQ=255)                                             ggTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca                                                                                               >  1:82346/1‑140 (MQ=255)                                             ggTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca                                                                                               >  1:870156/1‑140 (MQ=255)                                               ttattttttCACTTTCGACTTC‑AAGGGAGACGGTATTCCCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                             <  2:1379943/135‑1 (MQ=255)                                               tGATATTTCCACTTTCGACTTC‑AAGCGAGAGGTTATCCCCGGCCCCCTGGGC‑GGGGGGTAGGTTATAATTTTTCTTTCCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                             <  2:2235434/140‑1 (MQ=255)                                                  tatTTTCACTTTCGACTTC‑AAGCGAGACGGTATTCCCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                          <  1:2193818/140‑1 (MQ=255)                                                        cACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATTGCGCGGCACACCAGCTagt                                                                                    >  1:1241269/1‑138 (MQ=255)                                                         aCTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                   >  1:1708121/1‑140 (MQ=255)                                                           tttCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                 >  1:204138/1‑140 (MQ=255)                                                            ttCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                                >  2:1656626/1‑140 (MQ=255)                                                            ttCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                                >  1:1470314/1‑140 (MQ=255)                                                             tCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                               >  1:1721538/1‑140 (MQ=255)                                                                aCTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                            >  2:2397705/1‑140 (MQ=255)                                                                     aaaGCGAGACGTTATTCCCGGCCCCCGGGCC‑GCGCGGCAGTTTATAAATTTTCTTTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                        <  2:1454336/139‑1 (MQ=255)                                                                         ggagaCGGTATTCCCCGGCCCCTGGCC‑GGGCGGCGGTTTATAAATTTTCTTTTTATGGAGAAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCa                                                                    <  2:204138/139‑1 (MQ=255)                                                                             aCGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTg                                                                >  1:1726363/1‑140 (MQ=255)                                                                               ggTATTCCCGGGCCCGTGGCC‑GCGCGGCAGGTTATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGca                                                              <  2:2015487/140‑1 (MQ=255)                                                                                  ttcccccGCCCCCTGGCC‑GCGCGGCAGTTTATAAATTTTTTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACaa                                                           <  1:212505/136‑1 (MQ=255)                                                                                   ttccccGGCCCCTGGTC‑GCGCGGCAGTTTATGAATTTTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAAt                                                          <  2:1984565/137‑1 (MQ=255)                                                                                      cccGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                       >  1:764492/1‑140 (MQ=255)                                                                                       ccGGCCCCTGGCC‑GCGCGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTc                                                      <  1:2414721/140‑1 (MQ=255)                                                                                          gCCCCTGGCC‑GCGGGGCAGGTTATGAATTTTTTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGTGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                   <  2:1473470/140‑1 (MQ=255)                                                                                          gCCCCTGGCC‑GCGCGTAATTTTGCGATTTTTTTGTTGAAGAAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                   <  2:658623/140‑1 (MQ=255)                                                                                           ccccTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAGTGTTCCcgc                                                  >  2:1605759/1‑140 (MQ=255)                                                                                           ccccTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                  >  1:561111/1‑140 (MQ=255)                                                                                           ccccTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                  >  1:160257/1‑140 (MQ=255)                                                                                           ccccTGGAC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                  >  2:1203088/1‑140 (MQ=255)                                                                                                ggTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                             >  2:59547/1‑140 (MQ=255)                                                                                                  cc‑gcgggGCGGTTTATAAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGcc                                           <  2:681769/139‑1 (MQ=255)                                                                                                  cc‑gcgggGCGGGTTATAAATTTTTTGTTCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGcc                                           <  2:1003828/139‑1 (MQ=255)                                                                                                      cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                        >  1:681769/1‑140 (MQ=255)                                                                                                      cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                        >  1:47396/1‑140 (MQ=255)                                                                                                          gCAGTTTATAAATATTCTGTTAAGGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                    <  1:1550370/140‑1 (MQ=255)                                                                                                          gCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                    >  1:1664851/1‑140 (MQ=255)                                                                                                           cgggTTATGAATTTTCTGTTTGTGCAGGGAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                   <  1:1168541/138‑1 (MQ=255)                                                                                                           cAGTTTATAAATTTTCTGTTCATGCAGGAAAACCCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                   <  2:20300/140‑1 (MQ=255)                                                                                                             gtttATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAg                                 <  2:710502/138‑1 (MQ=255)                                                                                                             ggTTATGAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAg                                 <  2:160257/140‑1 (MQ=255)                                                                                                                  aaaaTATTCTTTTCAGGAAGGAAAAATCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                            <  2:1800414/138‑1 (MQ=255)                                                                                                                      tatTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                        <  1:80950/140‑1 (MQ=255)                                                                                                                       atTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                       >  1:109733/1‑140 (MQ=255)                                                                                                                        ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                      >  2:1218164/1‑140 (MQ=255)                                                                                                                        ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                      >  1:1813462/1‑140 (MQ=255)                                                                                                                        ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                      >  1:1800414/1‑140 (MQ=255)                                                                                                                         tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                     >  1:2374554/1‑140 (MQ=255)                                                                                                                         tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                     >  2:1870843/1‑140 (MQ=255)                                                                                                                            gTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                  >  2:182209/1‑140 (MQ=255)                                                                                                                             ttaagggaGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                 <  2:561111/133‑1 (MQ=255)                                                                                                                             ttCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                 <  2:1029731/140‑1 (MQ=255)                                                                                                                                aTGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg              >  1:279021/1‑140 (MQ=255)                                                                                                                                     gggAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgag         >  2:2039535/1‑140 (MQ=255)                                                                                                                                     gggAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgag         >  2:1561988/1‑140 (MQ=255)                                                                                                                                         aaaaCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACtt     <  1:2039535/140‑1 (MQ=255)                                                                                                                                           aaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc   >  1:1519483/1‑140 (MQ=255)                                                                                                                                            aCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCg  >  2:424802/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                            ACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTC‑AAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCG  >  NC_000913/3556068‑3556344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑