Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,538,2110GT54.9% 2.7 / 248.9 133F185L (TTC→TTA) nanMN‑acetylneuraminate mutarotase
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base T (41/32);  total (72/61)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 139 total aligned reads displayed.

TGGTTTGGCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAGTAACATAAGC  >  NC_000913/4538073‑4538348
                                                                                                                                            |                                                                                                                                         
tgttttGCCTCCCCCATAAAAAAGCAGGT‑TTTTACCCCTTTTATC‑CACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTga                                                                                                                                          <  2:2384482/137‑1 (MQ=255)
  ttttGCCTTCCCCATTAATAAGCCGGT‑TTTTATCCCTTTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTtaag                                                                                                                                        <  1:1538378/139‑1 (MQ=255)
  tttggtcTTCGCAA‑TAAAAAGGCAGG‑TTTTTCCCCCTTTTTTCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaag                                                                                                                                        <  2:2005224/134‑1 (MQ=255)
     tGGCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGaaa                                                                                                                                     >  1:124682/1‑140 (MQ=255)
       gCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaata                                                                                                                                   >  1:2494327/1‑140 (MQ=255)
       gCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaata                                                                                                                                   >  2:1983402/1‑140 (MQ=255)
         ttCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaataat                                                                                                                                 >  2:1405003/1‑140 (MQ=255)
            gCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtctt                                                                                                                              >  1:1361221/1‑140 (MQ=255)
                     aaGCCGGT‑TTTTACCCCCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttttt                                                                                                                     <  2:1173134/140‑1 (MQ=255)
                        ccaagtttttACCCCTTTTATC‑CACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTg                                                                                                                   <  1:2404443/134‑1 (MQ=255)
                        ccAGGTTTTTACCCCTTTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTg                                                                                                                   <  2:663442/140‑1 (MQ=255)
                        ccAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGt                                                                                                                  >  2:261764/1‑140 (MQ=255)
                          cattttttACCCCTTTTTTA‑CAAAACCGCCGCCCCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTc                                                                                                                 <  2:1403440/136‑1 (MQ=255)
                           ggtttttATCCCTTTTATC‑CACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCa                                                                                                                <  2:2193921/137‑1 (MQ=255)
                           gg‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCaa                                                                                                               >  2:2383655/1‑140 (MQ=255)
                            g‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaa                                                                                                              >  2:2270209/1‑140 (MQ=255)
                             tttttATCCCCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaa                                                                                                              <  1:940488/139‑1 (MQ=255)
                                  tccccttttATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGt                                                                                                         <  2:1395217/134‑1 (MQ=255)
                                  aTCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGt                                                                                                         >  1:1058005/1‑140 (MQ=255)
                                       ttttATT‑CACAACCGCCGCACCAGCCGTTCCGTGCCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCa                                                                                                    <  1:2146522/139‑1 (MQ=255)
                                       ttttATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCa                                                                                                    <  1:1032513/139‑1 (MQ=255)
                                       cTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTc                                                                                                                 <  1:1046691/127‑1 (MQ=255)
                                       cTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCa                                                                                                    >  1:2086750/1‑140 (MQ=255)
                                        tttttcccacaCCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCa                                                                                                    <  2:1357172/133‑1 (MQ=255)
                                        tttATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAt                                                                                                   <  1:1853131/140‑1 (MQ=255)
                                        tttATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAt                                                                                                   <  2:2494327/140‑1 (MQ=255)
                                             acaaaacccgccgCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAt                                                                                               <  2:1337583/133‑1 (MQ=255)
                                                acaACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAtttt                                                                                            <  1:261764/140‑1 (MQ=255)
                                                  aaCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTAt                                                                                          >  2:794115/1‑140 (MQ=255)
                                                  aaCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAt                                                                                                   <  2:128313/131‑1 (MQ=255)
                                                    ccgccgCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAt                                                                                               >  1:2420812/1‑133 (MQ=255)
                                                    ccgccgCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAt                                                                                               <  2:2420812/133‑1 (MQ=255)
                                                      gccgcACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCtat                                                                                      >  2:1972440/1‑140 (MQ=255)
                                                      gccgcACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCtat                                                                                      >  2:64371/1‑140 (MQ=255)
                                                       ccgcACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCtata                                                                                     >  2:911256/1‑140 (MQ=255)
                                                       ccgcACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCtata                                                                                     >  2:342387/1‑140 (MQ=255)
                                                        cgcACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAg                                                                                    <  1:904788/140‑1 (MQ=255)
                                                        cgcACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAg                                                                                    <  1:1146935/140‑1 (MQ=255)
                                                               gCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGa                                                                             >  2:1136428/1‑140 (MQ=255)
                                                                          ccAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAg                                                                  <  1:2205702/140‑1 (MQ=255)
                                                                          ccAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAg                                                                  <  2:124682/140‑1 (MQ=255)
                                                                          ccAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAg                                                                  >  1:2259161/1‑140 (MQ=255)
                                                                           cAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGc                                                                 <  1:342387/140‑1 (MQ=255)
                                                                            aGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAGCTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGcc                                                                <  2:419482/140‑1 (MQ=255)
                                                                            aGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGcc                                                                <  2:1372982/140‑1 (MQ=255)
                                                                             gggCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCt                                                               >  2:7794/1‑140 (MQ=255)
                                                                                  aTTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTg                                                          >  1:1304043/1‑140 (MQ=255)
                                                                                   ttCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTga                                                         >  1:21716/1‑140 (MQ=255)
                                                                                   ttCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTga                                                         >  1:1383647/1‑140 (MQ=255)
                                                                                    tCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTgag                                                        <  1:7794/140‑1 (MQ=255)
                                                                                    tCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTgag                                                        <  2:1304043/140‑1 (MQ=255)
                                                                                    tCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTgag                                                        <  1:1136428/140‑1 (MQ=255)
                                                                                     cGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTgaga                                                       >  2:633432/1‑140 (MQ=255)
                                                                                                 tCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAg                                           <  1:2442538/140‑1 (MQ=255)
                                                                                                 tCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAg                                           <  2:2259161/140‑1 (MQ=255)
                                                                                                     ttGCTGTGTTGAGGTATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCCGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCCGCCTCGTTGAGATCTTCGAAATAGCCAt                                       <  2:510424/140‑1 (MQ=255)
                                                                                                     ttGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCAt                                       >  1:1671526/1‑140 (MQ=255)
                                                                                                     ttGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCAt                                       >  2:892698/1‑140 (MQ=255)
                                                                                                     ttGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCAt                                       >  1:487377/1‑140 (MQ=255)
                                                                                                     ttGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCAt                                       <  1:633432/140‑1 (MQ=255)
                                                                                                     ttGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCAt                                       <  2:2426586/140‑1 (MQ=255)
                                                                                                     ttGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCAt                                       <  1:911256/140‑1 (MQ=255)
                                                                                                     ttGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCAt                                       >  2:1784639/1‑140 (MQ=255)
                                                                                                      tGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCAtt                                      <  2:1212650/140‑1 (MQ=255)
                                                                                                        cTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGa                                    <  2:851396/140‑1 (MQ=255)
                                                                                                        cTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGa                                    <  2:1361221/140‑1 (MQ=255)
                                                                                                          gtgtTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAg                                  <  2:1058005/140‑1 (MQ=255)
                                                                                                           tgtTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGa                                 >  2:1293991/1‑140 (MQ=255)
                                                                                                           tgtTGAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGa                                 >  2:2442338/1‑140 (MQ=255)
                                                                                                           tgtTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGa                                 >  2:1208915/1‑140 (MQ=255)
                                                                                                             ttGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGata                               >  1:2439789/1‑140 (MQ=255)
                                                                                                               gAGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATAtt                             <  1:1293991/140‑1 (MQ=255)
                                                                                                                aGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTc                            >  2:1099594/1‑140 (MQ=255)
                                                                                                                aGGGATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTCCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGa                                    >  1:315909/1‑132 (MQ=255)
                                                                                                                  ggATCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTg                          >  1:438456/1‑140 (MQ=255)
                                                                                                                     tCAAAAGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGtt                       >  2:392114/1‑140 (MQ=255)
                                                                                                                       aaaaGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTaa                     >  2:974918/1‑140 (MQ=255)
                                                                                                                       aaaaGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTaa                     >  2:954178/1‑140 (MQ=255)
                                                                                                                         aaGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAaca                   <  2:487040/140‑1 (MQ=255)
                                                                                                                          aGACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAacac                  <  2:1560260/140‑1 (MQ=255)
                                                                                                                           gACAACAGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAAcacc                 <  2:1383647/140‑1 (MQ=255)
                                                                                                                             caacaGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAAcaccac               <  2:21716/140‑1 (MQ=255)
                                                                                                                             caacaGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAAcaccac               >  1:1312124/1‑140 (MQ=255)
                                                                                                                              aacaGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAAcacca                >  2:1580620/1‑138 (MQ=255)
                                                                                                                              aacaGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAAcacca                <  1:1580620/138‑1 (MQ=255)
                                                                                                                              aacaGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACaccacc              >  2:102055/1‑140 (MQ=255)
                                                                                                                               acaGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACAccacca             <  1:1160660/140‑1 (MQ=255)
                                                                                                                               acaGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGGATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACAccacca             >  1:623957/1‑140 (MQ=255)
                                                                                                                               acaGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACAccacca             >  1:1849491/1‑140 (MQ=255)
                                                                                                                               acaGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACAccacca             <  2:2335134/140‑1 (MQ=255)
                                                                                                                                caGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACaccacctg            <  1:794115/140‑3 (MQ=255)
                                                                                                                                caGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAg            <  1:892698/140‑1 (MQ=255)
                                                                                                                                caGAAACTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAg            >  1:1348358/1‑140 (MQ=255)
                                                                                                                                caGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAg            >  1:1260771/1‑140 (MQ=255)
                                                                                                                                caGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAg            <  2:1260771/140‑1 (MQ=255)
                                                                                                                                caGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAg            <  1:1405003/140‑1 (MQ=255)
                                                                                                                                 aGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAGt           <  1:447395/140‑1 (MQ=255)
                                                                                                                                   aaaCTTATTTAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAGTaa         >  1:185608/1‑140 (MQ=255)
                                                                                                                                   aaaCTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAGTaa         >  2:1187902/1‑140 (MQ=255)
                                                                                                                                          ttGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAGTAACATAAGc  >  2:1000358/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TGGTTTGGCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTATAGCGGTTGAATCTTTTCCAGCCTCGTTGAGATCTTCAAAATAGCCATTGAAGATATTCTGGTTAACACCACCAGTAACATAAGC  >  NC_000913/4538073‑4538348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑